Helical reconstruction of CHIKV nsP3 helical scaffolds
Kril V, Hons M, Amadori C, Zimberger C, Couture L, Bouery Y, Burlaud-Gaillard J, Karpov A, Ptchelkine D, Thienel AL, Kummerer BM, Desfosses A, Jones R, Roingeard P, Meertens L, Amara A, Reguera J
Nat Commun (2024) 15 pp. 8106-8106 [ Pubmed: 39285216 DOI: doi:10.1038/s41467-024-51952-z ]
Helical reconstruction of CHIKV nsP3 helical scaffolds
Kril V, Hons M, Amadori C, Zimberger C, Couture L, Bouery Y, Burlaud-Gaillard J, Karpov A, Ptchelkine D, Thienel AL, Kummerer BM, Desfosses A, Jones R, Roingeard P, Meertens L, Amara A, Reguera J
Nat Commun (2024) 15 pp. 8106-8106 [ Pubmed: 39285216 DOI: doi:10.1038/s41467-024-51952-z ]
Kril V, Hons M, Amadori C, Zimberger C, Couture L, Bouery Y, Burlaud-Gaillard J, Karpov A, Ptchelkine D, Thienel AL, Kummerer BM, Desfosses A, Jones R, Roingeard P, Meertens L, Amara A, Reguera J
Nat Commun (2024) 15 pp. 8106-8106 [ DOI: doi:10.1038/s41467-024-51952-z Pubmed: 39285216 ]
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 monomeric assembly.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rj9
Q-score: 0.542
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 monomeric assembly. (Complex)
- Sgrna (Complex from Streptococcus thermophilus)
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
- Ntpam (Complex from Streptococcus virus 2972)
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Acriia6 (Complex from Streptococcus phage D1811)
Cryo-EM structure of St1Cas9-sgRNA-tDNA20-AcrIIA6 dimeric assembly.
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6rja
Q-score: 0.508
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ Pubmed: 31604602 DOI: doi:10.1016/j.molcel.2019.09.012 ]
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Tdna20 (6 kDa, DNA from Streptococcus virus 2972)
- Cryo-em structure of st1cas9-sgrna-tdna20-acriia6 dimeric assembly. (Complex)
- Crispr-associated endonuclease cas9 (Complex from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Acriia6 (Complex from Streptococcus phage D1811)
- Crispr-associated endonuclease cas9 1 (129 kDa, Protein from Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9))
- Rna (78-mer) (Complex from Streptococcus thermophilus)
- Rna (78-mer) (37 kDa, RNA from Streptococcus thermophilus)
- Tdna20 (Complex from Streptococcus virus 2972)
Cryo-EM structure of St1Cas9-sgRNA-tDNA59-ntPAM complex.
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6rjd
Q-score: 0.5
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Sgrna (78-mer) (37 kDa, RNA from Streptococcus thermophilus DGCC 7710)
- Tdna59 (Complex from Streptococcus phage D1811)
- Cryo-em structure of st1cas9-sgrna-tdna59-ntpam complex. (Complex)
- Ntpam (Complex from Streptococcus phage 2972)
- Streptococcus thermophilus 1 cas9 (129 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Streptococcus thermophilus 1 cas9 (Complex from Streptococcus thermophilus DGCC 7710)
- Sgrna (78-mer) (Complex from Streptococcus thermophilus)
- Tdna59 (18 kDa, DNA from Streptococcus phage D1811)
- Ntpam (7 kDa, DNA from Streptococcus Phage 2972)
Cryo-EM structure of St1Cas9-sgRNA-AcrIIA6-tDNA59-ntPAM complex.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6rjg
Q-score: 0.522
Fuchsbauer O, Swuec P, Zimberger C, Amigues B, Levesque S, Agudelo D, Duringer A, Chaves-Sanjuan A, Spinelli S, Rousseau GM, Velimirovic M, Bolognesi M, Roussel A, Cambillau C, Moineau S, Doyon Y, Goulet A
Mol Cell (2019) 76 pp. 922- [ DOI: doi:10.1016/j.molcel.2019.09.012 Pubmed: 31604602 ]
- Sgrna (Complex from Streptococcus thermophilus)
- Tdna59 (18 kDa, DNA from Streptococcus virus 2972)
- Ntpam (Complex from Streptococcus virus 2972)
- Cas 9 (Complex from Streptococcus thermophilus DGCC 7710)
- Acriia6 (21 kDa, Protein from Streptococcus phage D1811)
- Sgrna (37 kDa, RNA from Streptococcus thermophilus)
- Cryo-em structure of st1cas9-sgrna-acriia6-tdna59-ntpam complex. (Complex)
- Tdna59 (Complex from Streptococcus virus 2972)
- Acriia6 (Complex from Streptococcus phage D1811)
- Cas 9 (129 kDa, Protein from Streptococcus thermophilus DGCC 7710)
- Ntpam (7 kDa, DNA from Streptococcus virus 2972)