PI 3-kinase alpha with nanobody 3-126
Resolution: 2.41 Å
EM Method: Single-particle
Fitted PDBs: 8dcp
Q-score: 0.584
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ DOI: doi:10.1073/pnas.2210769119 Pubmed: 36095215 ]
- Water (18 Da, Ligand)
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-126 bound (Complex from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
PI 3-kinase alpha with nanobody 3-159
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8dcx
Q-score: 0.345
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ DOI: doi:10.1073/pnas.2210769119 Pubmed: 36095215 ]
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-159 bound (Complex from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
PI 3-kinase alpha with nanobody 3-142
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8dd4
Q-score: 0.42
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ DOI: doi:10.1073/pnas.2210769119 Pubmed: 36095215 ]
- Water (18 Da, Ligand)
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-142 bound (Complex from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
PI 3-kinase alpha with nanobody 3-142, crosslinked with DSG
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8dd8
Q-score: 0.454
Hart JR, Liu X, Pan C, Liang A, Ueno L, Xu Y, Quezada A, Zou X, Yang S, Zhou Q, Schoonooghe S, Hassanzadeh-Ghassabeh G, Xia T, Shui W, Yang D, Vogt PK, Wang MW
PNAS (2022) 119 pp. e2210769119-e2210769119 [ DOI: doi:10.1073/pnas.2210769119 Pubmed: 36095215 ]
- Human pi 3-kinase alpha complex composed of p110alpha and p85alpha with nanobody 3-142 bound (Complex from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
Cryo-EM structure of the SV1-Gs complex.
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7v9l
Q-score: 0.505
Cong Z, Zhou F, Zhang C, Zou X, Zhang H, Wang Y, Zhou Q, Cai X, Liu Q, Li J, Shao L, Mao C, Wang X, Wu J, Xia T, Zhao LH, Jiang H, Zhang Y, Xu HE, Cheng X, Yang D, Wang MW
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2106606118 Pubmed: 34599099 ]
- Nanobody 35 (15 kDa, Protein from synthetic construct)
- Cryo-em structure of the human growth hormone releasing hormone receptor splice variant 1 in complex with g protein (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (41 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Ghrh receptor splice variant 1,ghrh receptor splice variant 1,ghrh receptor splice variant 1,sv1 (57 kDa, Protein from Homo sapiens)
Cryo-EM Structure of p110alpha in complex with p85alpha
Resolution: 2.79 Å
EM Method: Single-particle
Fitted PDBs: 7myn
Q-score: 0.472
Liu X, Yang S, Hart JR, Xu Y, Zou X, Zhang H, Zhou Q, Xia T, Zhang Y, Yang D, Wang MW, Vogt PK
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2109327118 Pubmed: 34725156 ]
- Water (18 Da, Ligand)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- Heterodimer of pik3ca with pik3r1 (200 kDa, Complex from Homo sapiens)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
Cryo-EM structure of p110alpha in complex with p85alpha inhibited by BYL-719
Resolution: 2.92 Å
EM Method: Single-particle
Fitted PDBs: 7myo
Q-score: 0.38
Liu X, Yang S, Hart JR, Xu Y, Zou X, Zhang H, Zhou Q, Xia T, Zhang Y, Yang D, Wang MW, Vogt PK
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2109327118 Pubmed: 34725156 ]
- Heterodimer of p110alpha with p85alpha (200 kDa, Complex from Homo sapiens)
- Phosphatidylinositol 3-kinase regulatory subunit alpha (83 kDa, Protein from Homo sapiens)
- (2s)-n~1~-{4-methyl-5-[2-(1,1,1-trifluoro-2-methylpropan-2-yl)pyridin-4-yl]-1,3-thiazol-2-yl}pyrrolidine-1,2-dicarboxamide (441 Da, Ligand)
- Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform (127 kDa, Protein from Homo sapiens)
Cryo-EM structure of the GHRH-bound human GHRHR splice variant 1 complex
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 7v9m
Q-score: 0.431
Cong Z, Zhou F, Zhang C, Zou X, Zhang H, Wang Y, Zhou Q, Cai X, Liu Q, Li J, Shao L, Mao C, Wang X, Wu J, Xia T, Zhao LH, Jiang H, Zhang Y, Xu HE, Cheng X, Yang D, Wang MW
PNAS (2021) 118 [ DOI: doi:10.1073/pnas.2106606118 Pubmed: 34599099 ]
- Somatoliberin (5 kDa, Protein from Homo sapiens)
- Cryo-em structure of the human growth hormone releasing hormone receptor splice variant 1 in complex with ghrh and g protein (Complex from Homo sapiens)
- Human growth hormone releasing hormone receptor splice variant 1(sv1) (57 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Bos taurus)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (40 kDa, Protein from Rattus norvegicus)
- Nanobody-35 (15 kDa, Protein from synthetic construct)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (45 kDa, Protein from Homo sapiens)