Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 9h9e
Q-score: 0.372
Hooda Y, Sente A, Judy RM, Smalinskaite L, Chew SP, Naydenova K, Malinauskas T, Hardwick SW, Chirgadze DY, Aricescu AR, Hegde RS
bioRxiv (2024) [ Pubmed: 39553992 DOI: doi:10.1101/2024.10.28.620708 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-1 (54 kDa, Protein from Homo sapiens)
- Complex of gamma-aminobutyric acid receptor subunit alpha-1 with assembly factor nacho/tmem35a (Complex from Homo sapiens)
- (1s)-2-{[{[(2r)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(palmitoyloxy)methyl]ethyl stearate (751 Da, Ligand)
- Novel acetylcholine receptor chaperone (20 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
cryo-EM structure of Doa10 in MSP1E3D1
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 8pd0
Q-score: 0.449
Botsch JJ, Junker R, Sorgenfrei M, Ogger PP, Stier L, von Gronau S, Murray PJ, Seeger MA, Schulman BA, Brauning B
Nat Commun (2024) 15 pp. 410-410 [ DOI: doi:10.1038/s41467-023-44670-5 Pubmed: 38195637 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Doa10 with ubc6 and sybody in msp1e3d1 (150 kDa, Complex from Saccharomyces cerevisiae)
- Erad-associated e3 ubiquitin-protein ligase doa10 (151 kDa, Protein from Saccharomyces cerevisiae)
cryo-EM structure of Doa10 with RING domain in MSP1E3D1
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 8pda
Q-score: 0.419
Botsch JJ, Junker R, Sorgenfrei M, Ogger PP, Stier L, von Gronau S, Murray PJ, Seeger MA, Schulman BA, Brauning B
Nat Commun (2024) 15 pp. 410-410 [ DOI: doi:10.1038/s41467-023-44670-5 Pubmed: 38195637 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- 1,2-dioleoyl-sn-glycero-3-phosphocholine (787 Da, Ligand)
- Doa10 with ubc6 and sybody in msp1e3d1 (150 kDa, Complex from Saccharomyces cerevisiae)
- Erad-associated e3 ubiquitin-protein ligase doa10 (151 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7qn5
Q-score: 0.58
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- Nanobody nb25 (13 kDa, Protein from Lama glama)
- Human full-length extrasynaptic alpha4beta3delta gaba(a)r in complex with nanobody nb25 (Complex from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-4 (61 kDa, Protein from Homo sapiens)
- 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (238 Da, Ligand)
- Decane (142 Da, Ligand)
- N-octane (114 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit delta (52 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Chloride ion (35 Da, Ligand)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7qn7
Q-score: 0.548
Sente A, Desai R, Naydenova K, Malinauskas T, Jounaidi Y, Miehling J, Zhou X, Masiulis S, Hardwick SW, Chirgadze DY, Miller KW, Aricescu AR
Nature (2022) 604 pp. 190-194 [ Pubmed: 35355020 DOI: doi:10.1038/s41586-022-04517-3 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit beta-3 (54 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Gamma-amino-butanoic acid (103 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit alpha-4 (61 kDa, Protein from Homo sapiens)
- Histamine (111 Da, Ligand)
- Hexadecane (226 Da, Ligand)
- Decane (142 Da, Ligand)
- Gamma-aminobutyric acid receptor subunit delta (52 kDa, Protein from Homo sapiens)
- Nanobody nb25 (13 kDa, Protein from Homo sapiens)
- Human full-length extrasynaptic alpha4beta3delta gaba(a)r in complex with gaba, histamine and nanobody nb25 (Complex from Homo sapiens)
- Water (18 Da, Ligand)
cryoEM Structure of Polycystin-2 in complex with cations and lipids
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 5mke
Q-score: 0.303
Wilkes M, Madej MG, Kreuter L, Rhinow D, Heinz V, De Sanctis S, Ruppel S, Richter RM, Joos F, Grieben M, Pike AC, Huiskonen JT, Carpenter EP, Kuhlbrandt W, Witzgall R, Ziegler C
Nat Struct Mol Biol (2017) 24 pp. 123-130 [ Pubmed: 28092368 DOI: doi:10.1038/nsmb.3357 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Polycystin-2 (109 kDa, Protein from Homo sapiens)
- 4-amino-5-cyclohexyl-3-hydroxy-pentanoic acid (215 Da, Ligand)
- Polycystin-2 (Complex from homo sapiens)
cryoEM Structure of Polycystin-2 in complex with calcium and lipids
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 5mkf
Q-score: 0.369
Wilkes M, Madej MG, Kreuter L, Rhinow D, Heinz V, De Sanctis S, Ruppel S, Richter RM, Joos F, Grieben M, Pike AC, Huiskonen JT, Carpenter EP, Kuhlbrandt W, Witzgall R, Ziegler C
Nat Struct Mol Biol (2017) 24 pp. 123-130 [ Pubmed: 28092368 DOI: doi:10.1038/nsmb.3357 ]
- 1,2-dipalmitoyl-sn-glycero-3-phosphate (647 Da, Ligand)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- Palmitic acid (256 Da, Ligand)
- Polycystin-2 (109 kDa, Protein from Homo sapiens)
- 4-amino-5-cyclohexyl-3-hydroxy-pentanoic acid (215 Da, Ligand)
- Polycystin-2 (Complex from Homo sapiens)