Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ Pubmed: 36253376 DOI: doi:10.1038/s41467-022-33860-2 ]
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ Pubmed: 36253376 DOI: doi:10.1038/s41467-022-33860-2 ]
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ Pubmed: 36253376 DOI: doi:10.1038/s41467-022-33860-2 ]
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ Pubmed: 36253376 DOI: doi:10.1038/s41467-022-33860-2 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bg24 mature fab heavy chain (13 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp41 (14 kDa, Protein from Human immunodeficiency virus 1)
- Bg24 mature fab light chain (11 kDa, Protein from Homo sapiens)
- Cryo-em map of bg24 fabs with an inferred germline light chain and 10-1074 fabs in complex with hiv-1 env immunogen bg505-sosipv4.1-gt1 containing the n276 gp120 glycan- class 1 (Complex from Human immunodeficiency virus 1)
- 10-1074 fab light chain (11 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (14 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (49 kDa, Protein from Human immunodeficiency virus 1)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ugq
Q-score: 0.47
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ Pubmed: 36253376 DOI: doi:10.1038/s41467-022-33860-2 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bg24 with an inferred germline cdrl1 fab heavy chain (13 kDa, Protein from Homo sapiens)
- Bg24 with an inferred germline cdrl1 fab light chain (11 kDa, Protein from Homo sapiens)
- 10-1074 fab light chain (11 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (14 kDa, Protein from Homo sapiens)
- Cryo-em map of bg24 fabs with an inferred germline cdrl1 and 10-1074 fabs in complex with hiv-1 env 6405-sosip.664 (Complex from Human immunodeficiency virus 1)
- Envelope glycoprotein gp41 (14 kDa, Protein from Human immunodeficiency virus 1)
- Envelope glycoprotein gp120 (50 kDa, Protein from Human immunodeficiency virus 1)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ugn
Q-score: 0.477
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ Pubmed: 36253376 DOI: doi:10.1038/s41467-022-33860-2 ]
- Bg24 inferred germline fab with germline cdr3s heavy chain (13 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 10-1074 fab light chain (11 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (50 kDa, Protein from Human immunodeficiency virus 1)
- 10-1074 fab heavy chain (14 kDa, Protein from Homo sapiens)
- Bg24 inferred germline fab with germline cdr3s light chain (11 kDa, Protein from Homo sapiens)
- Cryo-em map of bg24-igl cdr3igl and 10-1074 fabs in complex with bg505 sosip.664v4.1-gt1 env trimer immunogen (Complex from Human immunodeficiency virus 1)
- Envelope glycoprotein gp41 (14 kDa, Protein from Human immunodeficiency virus 1)
- Alpha-d-mannopyranose (180 Da, Ligand)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 7ugo
Q-score: 0.425
Dam KA, Barnes CO, Gristick HB, Schoofs T, Gnanapragasam PNP, Nussenzweig MC, Bjorkman PJ
Nat Commun (2022) 13 pp. 6123-6123 [ Pubmed: 36253376 DOI: doi:10.1038/s41467-022-33860-2 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Cryo-em map of bg24-igl cdr3mat and 10-1074 fabs in complex with bg505 sosip.664v4.1-gt1 env trimer immunogen (Complex from Human immunodeficiency virus 1)
- Alpha-d-mannopyranose (180 Da, Ligand)
- 10-1074 fab light chain (11 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (14 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
- Bg24 inferred germline fab with mature cdr3s light chain (11 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp41 (14 kDa, Protein from Human immunodeficiency virus 1)
- Bg24 inferred germline fab with mature cdr3s heavy chain (13 kDa, Protein from Homo sapiens)
HIV-1 bNAb 1-18 in complex with BG505 SOSIP.664 and 10-1074
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6udj
Q-score: 0.632
Schommers P, Gruell H, Abernathy ME, Tran MK, Dingens AS, Gristick HB, Barnes CO, Schoofs T, Schlotz M, Vanshylla K, Kreer C, Weiland D, Holtick U, Scheid C, Valter MM, van Gils MJ, Sanders RW, Vehreschild JJ, Cornely OA, Lehmann C, Fatkenheuer G, Seaman MS, Bloom JD, Bjorkman PJ, Klein F
Cell (2020) 180 pp. 471- [ DOI: doi:10.1016/j.cell.2020.01.010 Pubmed: 32004464 ]
- 10-1074 fab light chain (23 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (25 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Bg505 sosip.664 trimer (Complex from Human immunodeficiency virus 1)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 10-1074 fab (Complex from Homo sapiens)
- 1-18 fab (Complex from Homo sapiens)
- 1-18 fab light chain (25 kDa, Protein from Homo sapiens)
- Complex of bnabs 1-18 and 10-1074 in complex with bg505 sosip.664 trimer (300 kDa, Complex)
- 1-18 fab heavy chain (53 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (53 kDa, Protein from Human immunodeficiency virus 1)
- Water (18 Da, Ligand)
HIV-1 bNAb 1-55 in complex with modified BG505 SOSIP-based immunogen RC1 and 10-1074
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6udk
Q-score: 0.442
Schommers P, Gruell H, Abernathy ME, Tran MK, Dingens AS, Gristick HB, Barnes CO, Schoofs T, Schlotz M, Vanshylla K, Kreer C, Weiland D, Holtick U, Scheid C, Valter MM, van Gils MJ, Sanders RW, Vehreschild JJ, Cornely OA, Lehmann C, Fatkenheuer G, Seaman MS, Bloom JD, Bjorkman PJ, Klein F
Cell (2020) 180 pp. 471- [ DOI: doi:10.1016/j.cell.2020.01.010 Pubmed: 32004464 ]
- 10-1074 fab light chain (23 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Rc1 variant of hiv-1 env glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rc1 variant of hiv-1 bg505 sosip.664 (Complex from Human immunodeficiency virus 1)
- 1-55 fab (Complex from Homo sapiens)
- 10-1074 fab (Complex from Homo sapiens)
- Complex of three bnab 1-55 fabs and three 10-1074 fabs bound to rc1 variant of bg505 sosip.664 trimer (300 kDa, Complex)
- 1-55 fab light chain (25 kDa, Protein from Homo sapiens)
- Rc1 variant of hiv-1 env glycoprotein gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- 1-55 fab heavy chain (28 kDa, Protein from Homo sapiens)
Modified BG505 SOSIP-based immunogen RC1 in complex with the elicited V3-glycan patch bNAb 10-1074
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 6orn
Q-score: 0.423
Escolano A, Gristick HB, Abernathy ME, Merkenschlager J, Gautam R, Oliveira TY, Pai J, West Jr AP, Barnes CO, Cohen AA, Wang H, Golijanin J, Yost D, Keeffe JR, Wang Z, Zhao P, Yao KH, Bauer J, Nogueira L, Gao H, Voll AV, Montefiori DC, Seaman MS, Gazumyan A, Silva M, McGuire AT, Stamatatos L, Irvine DJ, Wells L, Martin MA, Bjorkman PJ, Nussenzweig MC
Nature (2019) 570 pp. 468-473 [ DOI: doi:10.1038/s41586-019-1250-z Pubmed: 31142836 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rc1 variant of hiv-1 env glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 10-1074 antibody fab light chain (23 kDa, Protein from Homo sapiens)
- 10-1074 fab (Complex from Homo sapiens)
- Rc1 variant of bg505 sosip.664 trimer (Complex from Human immunodeficiency virus 1)
- Complex of rc1 variant of bg505 sosip.664 trimer with three 10-1074 fabs and three cd4bs fabs (600 kDa, Complex from Human immunodeficiency virus 1)
- 10-1074 antibody fab heavy chain (25 kDa, Protein from Homo sapiens)
- Rc1 variant of hiv-1 env glycoprotein gp120 (54 kDa, Protein from Human immunodeficiency virus 1)