Cryo-EM structure of the HIV-1 BG505 IDL Env trimer in complex with 3BNC117 and 10-1074 Fabs
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9bew
Q-score: 0.488
Zhang P, Gorman J, Tsybovsky Y, Lu M, Liu Q, Gopan V, Singh M, Lin Y, Miao H, Seo Y, Kwon A, Olia AS, Chuang GY, Geng H, Lai YT, Zhou T, Mascola JR, Mothes W, Kwong PD, Lusso P
Cell Rep (2024) 43 pp. 114518-114518 [ Pubmed: 39028623 DOI: doi:10.1016/j.celrep.2024.114518 ]
- 3bnc117 heavy chain (24 kDa, Protein from Homo sapiens)
- 10-1074 light chain (23 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 10-1074 heavy chain (25 kDa, Protein from Homo sapiens)
- 3bnc117 light chain (23 kDa, Protein from Homo sapiens)
- Cryo-em structure of the hiv-1 bg505 idl env trimer in complex with 3bnc117 and 10-1074 fabs (Complex from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7ucg
Q-score: 0.411
Barnes CO, Schoofs T, Gnanapragasam PNP, Golijanin J, Huey-Tubman KE, Gruell H, Schommers P, Suh-Toma N, Lee YE, Cetrulo Lorenzi JC, Piechocka-Trocha A, Scheid JF, West Jr AP, Walker BD, Seaman MS, Klein F, Nussenzweig MC, Bjorkman PJ
Sci Adv (2022) 8 pp. eabp8155-eabp8155 [ Pubmed: 35960796 DOI: doi:10.1126/sciadv.abp8155 ]
- 10-1074 fab heavy chain (26 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Complex of du422 sosip trimer bound to 10-1074 and bg24 fab fragments (650 kDa, Complex from Homo sapiens)
- 10-1074 light chain (23 kDa, Protein from Homo sapiens)
- Bg24 light chain (21 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Bg24 cdrh2-v2 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp160 (56 kDa, Protein from Human immunodeficiency virus 1)
VRC03 and 10-1074 Bound BG505 F14 HIV-1 SOSIP Envelope Trimer Structure
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6v8z
Q-score: 0.358
Henderson R, Lu M, Zhou Y, Mu Z, Parks R, Han Q, Hsu AL, Carter E, Blanchard SC, Edwards RJ, Wiehe K, Saunders KO, Borgnia MJ, Bartesaghi A, Mothes W, Haynes BF, Acharya P, Munir Alam S
Nat Commun (2020) 11 pp. 520-520 [ Pubmed: 31980614 DOI: doi:10.1038/s41467-019-14196-w ]
- Envelope glycoprotein gp41 (16 kDa, Protein from Human immunodeficiency virus 1)
- Bg505 f14/vt8 hiv-1 sosip env trimer (Complex from Human immunodeficiency virus 1)
- 10-1074 (Complex from Homo sapiens)
- 10-1074 fab light chain (22 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (52 kDa, Protein from Human immunodeficiency virus 1)
- Complex between vrc03, 10-1074, and bg505 f14 hiv-1 sosip env trimer (Complex)
- Vrc03 fab heavy chain (25 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Vrc03 fab light chain (23 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Vrc03 (Complex from Homo sapiens)
HIV-1 bNAb 1-18 in complex with BG505 SOSIP.664 and 10-1074
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 6udj
Q-score: 0.632
Schommers P, Gruell H, Abernathy ME, Tran MK, Dingens AS, Gristick HB, Barnes CO, Schoofs T, Schlotz M, Vanshylla K, Kreer C, Weiland D, Holtick U, Scheid C, Valter MM, van Gils MJ, Sanders RW, Vehreschild JJ, Cornely OA, Lehmann C, Fatkenheuer G, Seaman MS, Bloom JD, Bjorkman PJ, Klein F
Cell (2020) 180 pp. 471- [ DOI: doi:10.1016/j.cell.2020.01.010 Pubmed: 32004464 ]
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Water (18 Da, Ligand)
- Complex of bnabs 1-18 and 10-1074 in complex with bg505 sosip.664 trimer (300 kDa, Complex)
- 1-18 fab heavy chain (53 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1-18 fab (Complex from Homo sapiens)
- 1-18 fab light chain (25 kDa, Protein from Homo sapiens)
- 10-1074 fab light chain (23 kDa, Protein from Homo sapiens)
- 10-1074 fab heavy chain (25 kDa, Protein from Homo sapiens)
- Bg505 sosip.664 trimer (Complex from Human immunodeficiency virus 1)
- 10-1074 fab (Complex from Homo sapiens)
- Envelope glycoprotein gp120 (53 kDa, Protein from Human immunodeficiency virus 1)
HIV-1 bNAb 1-55 in complex with modified BG505 SOSIP-based immunogen RC1 and 10-1074
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6udk
Q-score: 0.442
Schommers P, Gruell H, Abernathy ME, Tran MK, Dingens AS, Gristick HB, Barnes CO, Schoofs T, Schlotz M, Vanshylla K, Kreer C, Weiland D, Holtick U, Scheid C, Valter MM, van Gils MJ, Sanders RW, Vehreschild JJ, Cornely OA, Lehmann C, Fatkenheuer G, Seaman MS, Bloom JD, Bjorkman PJ, Klein F
Cell (2020) 180 pp. 471- [ DOI: doi:10.1016/j.cell.2020.01.010 Pubmed: 32004464 ]
- Rc1 variant of hiv-1 bg505 sosip.664 (Complex from Human immunodeficiency virus 1)
- Complex of three bnab 1-55 fabs and three 10-1074 fabs bound to rc1 variant of bg505 sosip.664 trimer (300 kDa, Complex)
- Rc1 variant of hiv-1 env glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- 1-55 fab light chain (25 kDa, Protein from Homo sapiens)
- 10-1074 fab light chain (23 kDa, Protein from Homo sapiens)
- 1-55 fab (Complex from Homo sapiens)
- 10-1074 fab (Complex from Homo sapiens)
- 10-1074 fab heavy chain (25 kDa, Protein from Homo sapiens)
- 1-55 fab heavy chain (28 kDa, Protein from Homo sapiens)
- Rc1 variant of hiv-1 env glycoprotein gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
Modified BG505 SOSIP-based immunogen RC1 in complex with the elicited V3-glycan patch bNAb 10-1074
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 6orn
Q-score: 0.423
Escolano A, Gristick HB, Abernathy ME, Merkenschlager J, Gautam R, Oliveira TY, Pai J, West Jr AP, Barnes CO, Cohen AA, Wang H, Golijanin J, Yost D, Keeffe JR, Wang Z, Zhao P, Yao KH, Bauer J, Nogueira L, Gao H, Voll AV, Montefiori DC, Seaman MS, Gazumyan A, Silva M, McGuire AT, Stamatatos L, Irvine DJ, Wells L, Martin MA, Bjorkman PJ, Nussenzweig MC
Nature (2019) 570 pp. 468-473 [ DOI: doi:10.1038/s41586-019-1250-z Pubmed: 31142836 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Rc1 variant of hiv-1 env glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- 10-1074 antibody fab light chain (23 kDa, Protein from Homo sapiens)
- 10-1074 antibody fab heavy chain (25 kDa, Protein from Homo sapiens)
- Rc1 variant of bg505 sosip.664 trimer (Complex from Human immunodeficiency virus 1)
- 10-1074 fab (Complex from Homo sapiens)
- Complex of rc1 variant of bg505 sosip.664 trimer with three 10-1074 fabs and three cd4bs fabs (600 kDa, Complex from Human immunodeficiency virus 1)
- Rc1 variant of hiv-1 env glycoprotein gp120 (54 kDa, Protein from Human immunodeficiency virus 1)
B41 SOSIP.664 in complex with the silent-face antibody SF12 and V3-targeting antibody 10-1074
Resolution: 3.28 Å
EM Method: Single-particle
Fitted PDBs: 6okp
Q-score: 0.468
Schoofs T, Barnes CO, Suh-Toma N, Golijanin J, Schommers P, Gruell H, West Jr AP, Bach F, Lee YE, Nogueira L, Georgiev IS, Bailer RT, Czartoski J, Mascola JR, Seaman MS, McElrath MJ, Doria-Rose NA, Klein F, Nussenzweig MC, Bjorkman PJ
Immunity (2019) 50 pp. 1513- [ Pubmed: 31126879 DOI: doi:10.1016/j.immuni.2019.04.014 ]
- Sf12 heavy chain,sf12 heavy chain (26 kDa, Protein from Homo sapiens)
- B41 sosip.664 trimer (Complex from Human immunodeficiency virus 1)
- Sf12 light chain,sf12 light chain (23 kDa, Protein from Homo sapiens)
- Envelope glycoprotein gp120 (57 kDa, Protein from Human immunodeficiency virus 1)
- Sf12 fab (Complex from Homo sapiens)
- 10-1074 fab (Complex from Homo sapiens)
- 10-1074 heavy chain,10-1074 heavy chain (26 kDa, Protein from Homo sapiens)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Envelope glycoprotein gp41 (17 kDa, Protein from Human immunodeficiency virus 1)
- Complex of b41 sosip.664 trimer with three sf12 fabs and one 10-1074 fab (540 kDa, Complex)
- 10-1074 light chain,10-1074 light chain (23 kDa, Protein from Homo sapiens)
Schoofs T, Barnes CO, Suh-Toma N, Golijanin J, Schommers P, Gruell H, West Jr AP, Bach F, Lee YE, Nogueira L, Georgiev IS, Bailer RT, Czartoski J, Mascola JR, Seaman MS, McElrath MJ, Doria-Rose NA, Klein F, Nussenzweig MC, Bjorkman PJ
Immunity (2019) 50 pp. 1513-1529.e9 [ Pubmed: 31126879 DOI: doi:10.1016/j.immuni.2019.04.014 ]