Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1B)
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 5lmo
Q-score: 0.281
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Adenosine-5'-monophosphate (347 Da, Ligand)
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Uridine-5'-monophosphate (324 Da, Ligand)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (12 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
- 30s-if1-if3-mrna pre-initiation complex (state-1b) (826 kDa, Complex)
- Guanosine-5'-monophosphate (363 Da, Ligand)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1C)
Resolution: 5.35 Å
EM Method: Single-particle
Fitted PDBs: 5lmp
Q-score: 0.213
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (12 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna pre-initiation complex (state-1c) (826 kDa, Complex)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 5lmq
Q-score: 0.326
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Trna (24 kDa, RNA from Escherichia coli)
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (13 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna-trna pre-initiation complex, open form (state-2a) (849 kDa, Complex)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2B)
Resolution: 4.45 Å
EM Method: Single-particle
Fitted PDBs: 5lmr
Q-score: 0.294
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus HB8)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus HB8)
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus HB8)
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus HB8)
- 30s-if1-if3-mrna-trna pre-initiation complex (state-2b) (849 kDa, Complex)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus HB8)
- Trnai (24 kDa, RNA from Thermus thermophilus HB8)
- Mrna (13 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus HB8)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus HB8)
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus HB8)
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2C)
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 5lms
Q-score: 0.231
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna-trna pre-initiation complex (state-2c) (849 kDa, Complex)
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Trnai (24 kDa, RNA from Escherichia coli)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (13 kDa, RNA from Thermus thermophilus)
- Zinc ion (65 Da, Ligand)
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)