Cryo-EM structure of late human 39S mitoribosome assembly intermediates, state 1
Resolution: 5.7 Å
EM Method: Single-particle
Fitted PDBs: 7oi6
Q-score: 0.28
Cheng J, Berninghausen O, Beckmann R
Nat Commun (2021) 12 pp. 4544-4544 [ Pubmed: 34315873 DOI: doi:10.1038/s41467-021-24818-x ]
- 39s ribosomal protein l30, mitochondrial (18 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l45, mitochondrial (35 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l34, mitochondrial (10 kDa, Protein from Homo sapiens)
- Mief1 upstream open reading frame protein (8 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l2, mitochondrial (33 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l47, mitochondrial (29 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l54, mitochondrial (15 kDa, Protein from Homo sapiens)
- Peptidyl-trna hydrolase ict1, mitochondrial (23 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l43, mitochondrial (23 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l28, mitochondrial (30 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 39s ribosomal protein l53, mitochondrial (12 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l9, mitochondrial (30 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l15, mitochondrial (33 kDa, Protein from Homo sapiens)
- Growth arrest and dna damage-inducible proteins-interacting protein 1 (25 kDa, Protein from Homo sapiens)
- Acyl carrier protein, mitochondrial (17 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l24, mitochondrial (24 kDa, Protein from Homo sapiens)
- 39s ribosomal protein s30, mitochondrial (50 kDa, Protein from Homo sapiens)
- Rrna methyltransferase 3, mitochondrial (47 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l18, mitochondrial (20 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l37, mitochondrial (48 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l13, mitochondrial (20 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l11, mitochondrial (20 kDa, Protein from Homo sapiens)
- Human 39s mitoribosome assembly intermediates, state 1 (Complex from Homo sapiens)
- 39s ribosomal protein l39, mitochondrial (38 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l27, mitochondrial (16 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l52, mitochondrial (13 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l20, mitochondrial (17 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l23, mitochondrial (17 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l44, mitochondrial (37 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l51, mitochondrial (15 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l49, mitochondrial (19 kDa, Protein from Homo sapiens)
- Gtp-binding protein 10 (42 kDa, Protein from Homo sapiens)
- Ribosomal protein 63, mitochondrial (12 kDa, Protein from Homo sapiens)
- Mitochondrial assembly of ribosomal large subunit protein 1 (26 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l4, mitochondrial (34 kDa, Protein from Homo sapiens)
- Mitochondrial val trna (22 kDa, RNA from Homo sapiens)
- 39s ribosomal protein l16, mitochondrial (28 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l50, mitochondrial (18 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l3, mitochondrial (38 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l10, mitochondrial (29 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l17, mitochondrial (20 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l14, mitochondrial (15 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l32, mitochondrial (21 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l41, mitochondrial (15 kDa, Protein from Homo sapiens)
- 39s ribosomal protein s18a, mitochondrial (22 kDa, Protein from Homo sapiens)
- 16s rrna (500 kDa, RNA from Homo sapiens)
- 39s ribosomal protein l19, mitochondrial (33 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 39s ribosomal protein l38, mitochondrial (44 kDa, Protein from Homo sapiens)
- 4'-phosphopantetheine (358 Da, Ligand)
- 39s ribosomal protein l42, mitochondrial (16 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx28 (59 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l22, mitochondrial (23 kDa, Protein from Homo sapiens)
- 39s ribosomal protein l21, mitochondrial (22 kDa, Protein from Homo sapiens)
Structure of bacterial 30S-IF1-IF3-mRNA translation pre-initiation complex (state-1C)
Resolution: 5.35 Å
EM Method: Single-particle
Fitted PDBs: 5lmp
Q-score: 0.213
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ DOI: doi:10.1016/j.cell.2016.08.074 Pubmed: 27662086 ]
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (12 kDa, RNA from Thermus thermophilus)
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna pre-initiation complex (state-1c) (826 kDa, Complex)
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2C)
Resolution: 5.1 Å
EM Method: Single-particle
Fitted PDBs: 5lms
Q-score: 0.231
Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V
Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]
- 30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s-if1-if3-mrna-trna pre-initiation complex (state-2c) (849 kDa, Complex)
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Trnai (24 kDa, RNA from Escherichia coli)
- Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (13 kDa, RNA from Thermus thermophilus)
- Zinc ion (65 Da, Ligand)
- 16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)