Structure of the HrpA-bound E. coli disome, Class I
Campbell A, Esser HF, Burroughs AM, Berninghausen O, Aravind L, Becker T, Green R, Beckmann R, Buskirk AR
Mol Cell (2025) [ DOI: doi:10.1016/j.molcel.2025.01.018 ]
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
- Vemp nascent chain (20 kDa, Protein from Vibrio alginolyticus)
- Large ribosomal subunit protein ul15 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli)
- Messenger rna (223 kDa, RNA from Vibrio alginolyticus)
- Small ribosomal subunit protein bs21 (8 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl28 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs6, fully modified isoform (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- A-site trna (24 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein ul23 (11 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)
- P-site trna (24 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- A-site trna (24 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us7 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul1 (24 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Atp-dependent rna helicase hrpa (152 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl17 (14 kDa, Protein from Escherichia coli)
- 23s rna (941 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl33 (6 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl27 (9 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul10 (17 kDa, Protein from Escherichia coli)
- Hrpa-bound e. coli disome (Complex)
- E. coli disome (Complex from Escherichia coli)
- Messenger rna (Complex from Vibrio alginolyticus)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
- P-site trna (24 kDa, RNA from Escherichia coli)
- Vemp nascent chain (Complex from Vibrio alginolyticus)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs16 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
Structure of the HrpA-bound E. coli disome, Class II
Campbell A, Esser HF, Burroughs AM, Berninghausen O, Aravind L, Becker T, Green R, Beckmann R, Buskirk AR
Mol Cell (2025) [ DOI: doi:10.1016/j.molcel.2025.01.018 ]
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
- Vemp nascent chain (20 kDa, Protein from Vibrio alginolyticus)
- Large ribosomal subunit protein ul15 (15 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31a (7 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs21 (8 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl28 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs6, fully modified isoform (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- A-site trna (24 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein ul23 (11 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- P-site trna (24 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- A-site trna (24 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us7 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us19 (10 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul1 (24 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Atp-dependent rna helicase hrpa (152 kDa, Protein from Escherichia coli)
- Atp-dependent rna helicase hrpa (149 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl17 (14 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl33 (6 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl27 (9 kDa, Protein from Escherichia coli)
- Hrpa-bound e. coli disome (Complex from Escherichia coli)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul10 (17 kDa, Protein from Escherichia coli)
- Messenger rna (17 kDa, RNA from Vibrio alginolyticus)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
- P-site trna (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs16 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli)
E. coli 70S-TEC complex in delivery state
Resolution: 3.17 Å
EM Method: Single-particle
Fitted PDBs: 9gr1
Q-score: 0.421
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ DOI: doi:10.1126/science.ado8476 Pubmed: 39607923 ]
- Small ribosomal subunit protein us13 (13 kDa, Protein from Escherichia coli BL21(DE3))
- Mrna in sd-asd duplex (3 kDa, RNA from synthetic construct)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli BL21(DE3))
- Phe-nh-trna(phe) a-site (24 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein bs21 (8 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli BL21(DE3))
- Phenylalanine (165 Da, Ligand)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli BL21(DE3))
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli BL21(DE3))
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli BL21(DE3))
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli BL21(DE3))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli BL21(DE3))
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein bs6, fully modified isoform (15 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us7 (20 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 23s ribosomal rna (948 kDa, RNA from Escherichia coli BL21(DE3))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli BL21(DE3))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli BL21(DE3))
- Mrna in ribosome channel (5 kDa, RNA from synthetic construct)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli BL21(DE3))
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 70s ribosome (Complex from Escherichia coli BL21(DE3))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli BL21(DE3))
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli BL21(DE3))
30S PIC (Pre-Initiation complex)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9guq
Q-score: 0.487
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ DOI: doi:10.1126/science.ado8476 Pubmed: 39607923 ]
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6, non-modified isoform (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit (Complex from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30S mRNA delivery complex TEC resolved (30S only)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 9gus
Q-score: 0.401
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ DOI: doi:10.1126/science.ado8476 Pubmed: 39607923 ]
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit (Complex from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30S mRNA delivery complex (closed-head)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 9guv
Q-score: 0.561
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ DOI: doi:10.1126/science.ado8476 Pubmed: 39607923 ]
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosome (Complex from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- Trna(fmet) p-site (24 kDa, RNA from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30S-TEC (TEC in expressome position) Inactive state 2
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 9guw
Q-score: 0.394
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ DOI: doi:10.1126/science.ado8476 Pubmed: 39607923 ]
- Tec (Complex from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- Template dna strand (11 kDa, DNA from synthetic construct)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit bound to tec (Complex from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- Non-template dna strand (12 kDa, DNA from synthetic construct)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
30S-TEC (TEC in expressome position) Inactive state 1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 9gux
Q-score: 0.374
Webster MW, Chauvier A, Rahil H, Graziadei A, Charles K, Miropolskaya N, Takacs M, Saint-Andre C, Rappsilber J, Walter NG, Weixlbaumer A
Science (2024) 386 pp. eado8476-eado8476 [ DOI: doi:10.1126/science.ado8476 Pubmed: 39607923 ]
- Tec (Complex from Escherichia coli K-12)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli K-12)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Mrna (17 kDa, RNA from synthetic construct)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal subunit boud to tec (Complex from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s1 (61 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- Small ribosomal subunit protein bs6 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- Non-template dna strand (11 kDa, DNA from synthetic construct)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- Template dna strand (11 kDa, DNA from synthetic construct)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
cryo-EM structure of Staphylococcus aureus(ATCC 29213) 70S ribosome in complex with MCX-190.
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8y39
Q-score: 0.353
Ma CX, Li Y, Liu WT, Li Y, Zhao F, Lian XT, Ding J, Liu SM, Liu XP, Fan BZ, Liu LY, Xue F, Li J, Zhang JR, Xue Z, Pei XT, Lin JZ, Liang JH
Cell Discov (2024) 10 pp. 75-75 [ Pubmed: 38992047 DOI: doi:10.1038/s41421-024-00702-y ]
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us10 (11 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul15 (15 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Staphylococcus aureus)
- 23s ribosomal rna (946 kDa, RNA from Staphylococcus aureus)
- 7-[4-[3-[[(1~{s},2~{r},5~{r},6~{s},7~{s},8~{r},9~{r},11~{r},13~{r},14~{r})-8-[(2~{s},3~{r},4~{s},6~{r})-4-(dimethylamino)-6-methyl-3-oxidanyl-oxan-2-yl]oxy-2-ethyl-9-methoxy-1,5,7,9,11,13-hexamethyl-4,12,16-tris(oxidanylidene)-3,17-dioxa-15-azabicyclo[12.3.0]heptadecan-6-yl]oxycarbonylamino]propoxy]but-1-ynyl]-1-methyl-4-oxidanylidene-quinoline-3-carboxylic acid (969 Da, Ligand)
- Staphylococcus aureus(atcc 29213) 70s ribosome in complex with mcx-190. (Complex from Staphylococcus aureus)
- Large ribosomal subunit protein ul5 (19 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us3 (24 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Staphylococcus aureus)
- 16s ribosomal rna (500 kDa, RNA from Staphylococcus aureus)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Staphylococcus aureus)
- 5s ribosomal rna (36 kDa, RNA from Staphylococcus aureus)
- Small ribosomal subunit protein bs6 (11 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us12 (16 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us17 (10 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us19 (12 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl36 (4 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us2 (26 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us13 (13 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl33b (5 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us14b (7 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl25 (10 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl32 (5 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul3 (23 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein bs16 (10 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Staphylococcus aureus)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein ul18 (13 kDa, Protein from Staphylococcus aureus)
- Small ribosomal subunit protein us7 (17 kDa, Protein from Staphylococcus aureus)
- Large ribosomal subunit protein bl31b (6 kDa, Protein from Staphylococcus aureus)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8fzd
Q-score: 0.552
Li L, Rybak MY, Lin J, Gagnon MG
Nat Struct Mol Biol (2024) 31 pp. 1909-1920 [ Pubmed: 39030416 DOI: doi:10.1038/s41594-024-01360-0 ]
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- Ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- P-site phenylalanyl-trna (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- F-uaa mrna (6 kDa, RNA from Escherichia phage T4)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- Peptide chain release factor 1 (40 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l10 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- P-site phenylalanyl-trna (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
- Peptide chain release factor rf3 (59 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Cryo-em structure of an e. coli non-rotated ribosome termination complex bound with aporf3, rf1, p- and e-site trnaphe (composite state i-b) (2 MDa, Complex from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)