26S proteasome Rpt1-RK -Ubp6-UbVS complex in the si state
Resolution: 6.0 Å
EM Method: Single-particle
Fitted PDBs: 7qo5
Q-score: 0.133
Hung KYS, Klumpe S, Eisele MR, Elsasser S, Tian G, Sun S, Moroco JA, Cheng TC, Joshi T, Seibel T, Van Dalen D, Feng XH, Lu Y, Ovaa H, Engen JR, Lee BH, Rudack T, Sakata E, Finley D
Nat Commun (2022) 13 pp. 838-838 [ DOI: doi:10.1038/s41467-022-28186-y Pubmed: 35149681 ]
- Proteasome subunit alpha type-2 (27 kDa, Protein from baker's yeast)
- Proteasome subunit alpha type-6 (25 kDa, Protein from baker's yeast)
- Proteasome subunit alpha type-1 (28 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit 7 homolog (52 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit 8 homolog (45 kDa, Protein from baker's yeast)
- Polyubiquitin-b (8 kDa, Protein from Homo sapiens)
- 26s proteasome regulatory subunit 4 homolog (48 kDa, Protein from baker's yeast)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 26s proteasome regulatory subunit rpn5 (51 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn12 (31 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn1 (109 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn7 (49 kDa, Protein from baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Yeast rpt1-rk 26s proteasome in si state (Complex from Saccharomyces cerevisiae)
- Proteasome subunit beta type-2 (28 kDa, Protein from baker's yeast)
- Proteasome subunit beta type-3 (22 kDa, Protein from baker's yeast)
- 26s proteasome rpt1-rk -ubp6-ubvs complex in the si state (Complex)
- Ubiquitin carboxyl-terminal hydrolase 6 (57 kDa, Protein from Saccharomyces cerevisiae)
- 26s proteasome regulatory subunit 6a (48 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn10 (29 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn6 (49 kDa, Protein from baker's yeast)
- Proteasome subunit alpha type-5 (28 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn13 (17 kDa, Protein from baker's yeast)
- Ubp6-ubvs complex (Complex from Saccharomyces cerevisiae)
- Proteasome subunit beta type-4 (22 kDa, Protein from baker's yeast)
- Ubiquitin carboxyl-terminal hydrolase rpn11 (34 kDa, Protein from baker's yeast)
- 26s proteasome subunit rpt4 (49 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn2 (104 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn9 (45 kDa, Protein from baker's yeast)
- 26s proteasome complex subunit sem1 (10 kDa, Protein from baker's yeast)
- Proteasome subunit beta type-5 (31 kDa, Protein from baker's yeast)
- Proteasome subunit beta type-7 (29 kDa, Protein from baker's yeast)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 26s proteasome regulatory subunit 6b homolog (47 kDa, Protein from baker's yeast)
- Probable proteasome subunit alpha type-7 (31 kDa, Protein from baker's yeast)
- Proteasome subunit beta type-6 (26 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn3 (60 kDa, Protein from baker's yeast)
- Proteasome subunit beta type-1 (23 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn8 (38 kDa, Protein from baker's yeast)
- Proteasome subunit alpha type-4 (28 kDa, Protein from baker's yeast)
- Proteasome subunit alpha type-3 (28 kDa, Protein from baker's yeast)
26S proteasome Rpt1-RK -Ubp6-UbVS complex in the s2 state
Resolution: 6.3 Å
EM Method: Single-particle
Fitted PDBs: 7qo6
Q-score: 0.096
Hung KYS, Klumpe S, Eisele MR, Elsasser S, Tian G, Sun S, Moroco JA, Cheng TC, Joshi T, Seibel T, Van Dalen D, Feng XH, Lu Y, Ovaa H, Engen JR, Lee BH, Rudack T, Sakata E, Finley D
Nat Commun (2022) 13 pp. 838-838 [ DOI: doi:10.1038/s41467-022-28186-y Pubmed: 35149681 ]
- 26s proteasome regulatory subunit 7 homolog (52 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit 8 homolog (45 kDa, Protein from baker's yeast)
- Polyubiquitin-b (8 kDa, Protein from Homo sapiens)
- 26s proteasome regulatory subunit 4 homolog (48 kDa, Protein from baker's yeast)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 26s proteasome regulatory subunit rpn5 (51 kDa, Protein from baker's yeast)
- Bj4_g0021480.mrna.1.cds.1 (28 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn1 (109 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn7 (49 kDa, Protein from baker's yeast)
- Magnesium ion (24 Da, Ligand)
- Bj4_g0020160.mrna.1.cds.1 (28 kDa, Protein from baker's yeast)
- Ubiquitin carboxyl-terminal hydrolase (57 kDa, Protein from Saccharomyces cerevisiae)
- 26s proteasome regulatory subunit 6a (48 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn10 (29 kDa, Protein from baker's yeast)
- Hlj1_g0013750.mrna.1.cds.1 (26 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn11 (34 kDa, Protein from baker's yeast)
- Proteasome subunit beta (22 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn6 (49 kDa, Protein from baker's yeast)
- Em14s01-3b_g0035190.mrna.1.cds.1 (28 kDa, Protein from baker's yeast)
- Em14s01-3b_g0050020.mrna.1.cds.1 (31 kDa, Protein from baker's yeast)
- 26s proteasome subunit rpt4 (49 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn2 (104 kDa, Protein from baker's yeast)
- Proteasome endopeptidase complex (22 kDa, Protein from baker's yeast)
- 26s proteasome rpt1-rk -ubp6-ubvs complex in the s2 state (2 MDa, Complex from Saccharomyces cerevisiae)
- Bj4_g0043800.mrna.1.cds.1 (25 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn9 (45 kDa, Protein from baker's yeast)
- 26s proteasome complex subunit sem1 (10 kDa, Protein from baker's yeast)
- Proteasome subunit beta type-5 (31 kDa, Protein from baker's yeast)
- Proteasome subunit beta type-7 (29 kDa, Protein from baker's yeast)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 26s proteasome regulatory subunit 6b homolog (47 kDa, Protein from baker's yeast)
- Probable proteasome subunit alpha type-7 (31 kDa, Protein from baker's yeast)
- Hlj1_g0039880.mrna.1.cds.1 (27 kDa, Protein from baker's yeast)
- Hlj1_g0030700.mrna.1.cds.1 (17 kDa, Protein from baker's yeast)
- Proteasome endopeptidase complex (28 kDa, Protein from baker's yeast)
- Hlj1_g0048980.mrna.1.cds.1 (28 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn3 (60 kDa, Protein from baker's yeast)
- Proteasome subunit beta type-1 (23 kDa, Protein from baker's yeast)
- 26s proteasome regulatory subunit rpn8 (38 kDa, Protein from baker's yeast)
yeast proteasome in Ub-engaged state (C2)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6j30
Q-score: 0.171
Ding Z, Xu C, Sahu I, Wang Y, Fu Z, Huang M, Wong CCL, Glickman MH, Cong Y
Mol Cell (2019) 73 pp. 1150- [ Pubmed: 30792173 DOI: doi:10.1016/j.molcel.2019.01.018 ]
- 26s proteasome regulatory subunit 4 homolog (48 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-2 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-4 (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-7 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome subunit rpt4 (49 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-5 (31 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn2 (104 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-1 (23 kDa, Protein from Saccharomyces cerevisiae S288c)
- Yeast proteasome (Complex from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-5 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn10 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ubiquitin carboxyl-terminal hydrolase rpn11 (34 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-4 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn8 (38 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn3 (60 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn7 (49 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-3 (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn9 (45 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-1 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn12 (31 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit 6b homolog (47 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn13 (17 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn6 (49 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit 6a (48 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit 8 homolog (45 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-6 (26 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn1 (109 kDa, Protein from Saccharomyces cerevisiae S288c)
- Probable proteasome subunit alpha type-7 (31 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit 7 homolog (52 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome complex subunit sem1 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn5 (51 kDa, Protein from Saccharomyces cerevisiae S288c)
Yeast proteasome in resting state (C1-a)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6j2x
Q-score: 0.242
Ding Z, Xu C, Sahu I, Wang Y, Fu Z, Huang M, Wong CCL, Glickman MH, Cong Y
Mol Cell (2019) 73 pp. 1150- [ Pubmed: 30792173 DOI: doi:10.1016/j.molcel.2019.01.018 ]
- Proteasome subunit beta type-2 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-4 (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Yeast 26s proteasome (Complex from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-7 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome subunit rpt4 (49 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-5 (31 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s protease regulatory subunit 4 homolog (48 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn2 (104 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-1 (23 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-5 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn10 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ubiquitin carboxyl-terminal hydrolase rpn11 (34 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-4 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn8 (38 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn5 (51 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn3 (60 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn7 (49 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-3 (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome component c5 (26 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn9 (45 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-1 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn12 (31 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit 6b homolog (47 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn13 (17 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn6 (49 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit 6a (48 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit 8 homolog (45 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit rpn1 (109 kDa, Protein from Saccharomyces cerevisiae S288c)
- Probable proteasome subunit alpha type-7 (31 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit 7 homolog (52 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome complex subunit sem1 (10 kDa, Protein from Saccharomyces cerevisiae S288c)
Yeast 26S proteasome bound to ubiquitinated substrate (1D* motor state)
Resolution: 4.43 Å
EM Method: Single-particle
Fitted PDBs: 6ef0
Q-score: 0.383
de la Pena AH, Goodall EA, Gates SN, Lander GC, Martin A
Science (2018) 362 [ DOI: doi:10.1126/science.aav0725 Pubmed: 30309908 ]
- 26s proteasome regulatory subunit 4 homolog (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-3 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 8 homolog (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 26s proteasome subunit rpt4 (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-5 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Substrate-engaged 26s proteasome in the 1d* state (Complex)
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Model substrate polypeptide (1 kDa, Protein from Homo sapiens)
- 26s proteasome regulatory subunit 6a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 6b homolog (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-1 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Proteasome subunit alpha type-4 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 7 homolog (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Probable proteasome subunit alpha type-7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Substrate (Complex from Homo sapiens)
- Proteasome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast 26S proteasome bound to ubiquitinated substrate (5D motor state)
Resolution: 4.73 Å
EM Method: Single-particle
Fitted PDBs: 6ef1
Q-score: 0.371
de la Pena AH, Goodall EA, Gates SN, Lander GC, Martin A
Science (2018) 362 [ DOI: doi:10.1126/science.aav0725 Pubmed: 30309908 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 26s proteasome regulatory subunit 4 homolog (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Substrate-engaged 26s proteasome in the 5d state (composite map) (Complex)
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-5 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome subunit rpt4 (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Model substrate polypeptide (1 kDa, Protein from Homo sapiens)
- Probable proteasome subunit alpha type-7 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 8 homolog (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-1 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 7 homolog (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 6a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Proteasome subunit alpha type-4 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-3 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 6b homolog (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Substrate (Complex from Homo sapiens)
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Yeast 26S proteasome bound to ubiquitinated substrate (5T motor state)
Resolution: 4.27 Å
EM Method: Single-particle
Fitted PDBs: 6ef2
Q-score: 0.43
de la Pena AH, Goodall EA, Gates SN, Lander GC, Martin A
Science (2018) 362 [ DOI: doi:10.1126/science.aav0725 Pubmed: 30309908 ]
- Substrate-engaged 26s proteasome in the 5t state (Complex)
- 26s proteasome regulatory subunit 6a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Model substrate polypeptide (1 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-1 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-4 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 8 homolog (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 4 homolog (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome subunit rpt4 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-5 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 6b homolog (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Proteasome subunit alpha type-3 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 7 homolog (30 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Probable proteasome subunit alpha type-7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Substrate (Complex from Homo sapiens)
Yeast 26S proteasome bound to ubiquitinated substrate (4D motor state)
Resolution: 4.17 Å
EM Method: Single-particle
Fitted PDBs: 6ef3
Q-score: 0.46
de la Pena AH, Goodall EA, Gates SN, Lander GC, Martin A
Science (2018) 362 [ DOI: doi:10.1126/science.aav0725 Pubmed: 30309908 ]
- 26s proteasome regulatory subunit 4 homolog (48 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-2 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-4 (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-7 (29 kDa, Protein from Saccharomyces cerevisiae S288c)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 26s proteasome subunit rpt4 (49 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-5 (31 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-7 (1 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-1 (23 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-5 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ubiquitin carboxyl-terminal hydrolase rpn11 (34 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-4 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-3 (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit alpha type-1 (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- Substrate (Complex from Homo sapiens)
- 26s proteasome regulatory subunit 6b homolog (47 kDa, Protein from Saccharomyces cerevisiae S288c)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 26s proteasome regulatory subunit 6a (48 kDa, Protein from Saccharomyces cerevisiae S288c)
- Substrate-engaged 26s proteasome in the 4d state (Complex)
- Model substrate polypeptide (4 kDa, Protein from Homo sapiens)
- 26s proteasome regulatory subunit 8 homolog (45 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome subunit beta type-6 (26 kDa, Protein from Saccharomyces cerevisiae S288c)
- Probable proteasome subunit alpha type-7 (31 kDa, Protein from Saccharomyces cerevisiae S288c)
- 26s proteasome regulatory subunit 7 homolog (52 kDa, Protein from Saccharomyces cerevisiae S288c)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6fvw
Q-score: 0.204
Eisele MR, Reed RG, Rudack T, Schweitzer A, Beck F, Nagy I, Pfeifer G, Plitzko JM, Baumeister W, Tomko Jr RJ, Sakata E
Cell Rep (2018) 24 pp. 1301-1315.e5 [ Pubmed: 30067984 DOI: doi:10.1016/j.celrep.2018.07.004 ]
- Proteasome subunit beta type-1 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 4 homolog (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 8 homolog (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Proteasome subunit beta type-4 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-7 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 6a (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Proteasome subunit alpha type-3 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Probable proteasome subunit alpha type-7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-1 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome complex subunit sem1 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn5 (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn1 (106 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn3 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-4 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn7 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-3 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome subunit rpt4 (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn13 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn6 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 6b homolog (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn2 (101 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin carboxyl-terminal hydrolase rpn11 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn8 (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-2 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Water (18 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Proteasome subunit alpha type-5 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-2 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-5 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 7 homolog (47 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn12 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn9 (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn10 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-6 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Resolution: 5.4 Å
EM Method: Single-particle
Fitted PDBs: 6fvv
Q-score: 0.149
Eisele MR, Reed RG, Rudack T, Schweitzer A, Beck F, Nagy I, Pfeifer G, Plitzko JM, Baumeister W, Tomko Jr RJ, Sakata E
Cell Rep (2018) 24 pp. 1301-1315.e5 [ Pubmed: 30067984 DOI: doi:10.1016/j.celrep.2018.07.004 ]
- Proteasome subunit beta type-1 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 4 homolog (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-5 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Proteasome subunit beta type-4 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-7 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-6 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 6a (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Probable proteasome subunit alpha type-7 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 6b homolog (44 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- 26s proteasome complex subunit sem1 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn5 (51 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn1 (106 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn3 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-3 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn7 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 8 homolog (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-3 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-4 (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit 7 homolog (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn13 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn6 (49 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome subunit rpt4 (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit alpha type-1 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn2 (101 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin carboxyl-terminal hydrolase rpn11 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn8 (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Proteasome subunit beta type-2 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-5 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn12 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn9 (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 26s proteasome regulatory subunit rpn10 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Proteasome subunit beta type-6 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))