60S ribosomal subunit bound to the E3-UFM1 complex (native, UFM1 pulldown)
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ DOI: doi:10.1038/s41586-024-07073-0 Pubmed: 38383785 ]
60S ribosomal subunit bound to the E3-UFM1 complex - state 3 (native)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8ohd
Q-score: 0.531
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ DOI: doi:10.1038/s41586-024-07073-0 Pubmed: 38383785 ]
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7 (29 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- Ddrgk domain-containing protein 1 (35 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l10a (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l8 (28 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (89 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- Ubiquitin-fold modifier 1 (9 kDa, Protein from Homo sapiens)
- 60s ribosomal subunit (Complex from Homo sapiens)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27a (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 28s rrna (1 MDa, RNA from Homo sapiens)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal subunit in complex with e3-ufm1 ligase (Complex)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (Complex from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- Cdk5 regulatory subunit-associated protein 3 (56 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens)
60S ribosomal subunit bound to the E3-UFM1 complex - state 2 (native)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8oj0
Q-score: 0.463
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ DOI: doi:10.1038/s41586-024-07073-0 Pubmed: 38383785 ]
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7 (29 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens environmental sample)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- Ddrgk domain-containing protein 1 (35 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l10a (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l8 (28 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (89 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- Ubiquitin-fold modifier 1 (9 kDa, Protein from Homo sapiens)
- 60s ribosomal subunit (Complex from Homo sapiens)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27a (16 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 28s rrna (1 MDa, RNA from Homo sapiens)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (Complex from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal subunit bound to the e3-ufm1 complex and sec61 (Complex)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- Cdk5 regulatory subunit-associated protein 3 (56 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
60S ribosomal subunit bound to the E3-UFM1 complex - state 3 (in-vitro reconstitution)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8oj5
Q-score: 0.57
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ DOI: doi:10.1038/s41586-024-07073-0 Pubmed: 38383785 ]
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7 (29 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- Ddrgk domain-containing protein 1 (35 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l10a (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l8 (28 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- E3 ufm1-protein ligase 1 (89 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- E3-ufm1 complex bound to the 60s ribosome (Complex from Homo sapiens)
- Ubiquitin-fold modifier 1 (9 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27a (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 28s rrna (1 MDa, RNA from Homo sapiens)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- Cdk5 regulatory subunit-associated protein 3 (56 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens)
60S ribosomal subunit bound to the E3-UFM1 complex - state 1 (native)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8oj8
Q-score: 0.468
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ DOI: doi:10.1038/s41586-024-07073-0 Pubmed: 38383785 ]
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7 (29 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l10a (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l8 (28 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (89 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Homo sapiens)
- 60s ribosomal subunit (Complex from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27a (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 28s rrna (1 MDa, RNA from Homo sapiens)
- 60s ribosomal subunit in complex with e3-ufm1 ligase, state 1 (Complex)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (Complex from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens)
Cryo-EM structure of native Otu2-bound ubiquitinated 43S pre-initiation complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8cah
Q-score: 0.43
Ikeuchi K, Ivic N, Buschauer R, Cheng J, Frohlich T, Matsuo Y, Berninghausen O, Inada T, Becker T, Beckmann R
Nat Commun (2023) 14 pp. 2730-2730 [ Pubmed: 37169754 DOI: doi:10.1038/s41467-023-38161-w ]
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae W303)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Sui1 isoform 1 (12 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
- Rna recognition motif (unknown) (6 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s4-b (29 kDa, Protein from Saccharomyces cerevisiae W303)
- Rli1 isoform 1 (68 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s3 (26 kDa, Protein from Saccharomyces cerevisiae W303)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s31 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 3 subunit c (93 kDa, Protein from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 3 subunit a (110 kDa, Protein from Saccharomyces cerevisiae W303)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 3 subunit g (30 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae W303)
- 60s ribosomal protein l41-a (3 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast native 43s pre-initiation complex with deubiquitinating enzyme otu2 bound to ubiquitinated es7 (Complex from Saccharomyces cerevisiae)
- Eukaryotic translation initiation factor 3 subunit j (29 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- Rps15 isoform 1 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae W303)
- 60s ribosomal protein l40-a (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Zinc ion (65 Da, Ligand)
- Otu domain-containing protein 2 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s26-a (13 kDa, Protein from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 3 subunit b (88 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s25-a (12 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s6-b (27 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s10-a (12 kDa, Protein from Saccharomyces cerevisiae W303)
- 18s ribosomal rna (579 kDa, RNA from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 4c (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Iron/sulfur cluster (351 Da, Ligand)
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 3 subunit i (38 kDa, Protein from Saccharomyces cerevisiae W303)
- Rps29a isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
Cryo-EM structure of native Otu2-bound ubiquitinated 48S initiation complex (partial)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8cas
Q-score: 0.366
Ikeuchi K, Ivic N, Buschauer R, Cheng J, Frohlich T, Matsuo Y, Berninghausen O, Inada T, Becker T, Beckmann R
Nat Commun (2023) 14 pp. 2730-2730 [ Pubmed: 37169754 DOI: doi:10.1038/s41467-023-38161-w ]
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae W303)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
- Methionine (149 Da, Ligand)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
- Trna (24 kDa, RNA from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s4-b (29 kDa, Protein from Saccharomyces cerevisiae W303)
- Rli1 isoform 1 (68 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s3 (26 kDa, Protein from Saccharomyces cerevisiae W303)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s31 (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast native 48s initiation complex (partial) with deubiquitinating enzyme otu2 bound to ubiquitinated es7 (Complex from Saccharomyces cerevisiae)
- Eukaryotic translation initiation factor 3 subunit c (93 kDa, Protein from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 3 subunit a (110 kDa, Protein from Saccharomyces cerevisiae W303)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 3 subunit g (30 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae W303)
- 60s ribosomal protein l41-a (3 kDa, Protein from Saccharomyces cerevisiae W303)
- Mrna (2 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- Rps15 isoform 1 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae W303)
- 60s ribosomal protein l40-a (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Sui2 isoform 1 (34 kDa, Protein from Saccharomyces cerevisiae W303)
- Zinc ion (65 Da, Ligand)
- Otu domain-containing protein 2 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s26-a (13 kDa, Protein from Saccharomyces cerevisiae W303)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 3 subunit b (88 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s25-a (12 kDa, Protein from Saccharomyces cerevisiae W303)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 40s ribosomal protein s6-b (27 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s10-a (12 kDa, Protein from Saccharomyces cerevisiae W303)
- 18s ribosomal rna (579 kDa, RNA from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 4c (17 kDa, Protein from Saccharomyces cerevisiae W303)
- Protein-synthesizing gtpase (57 kDa, Protein from Saccharomyces cerevisiae W303)
- Tif5 isoform 1 (45 kDa, Protein from Saccharomyces cerevisiae W303)
- Iron/sulfur cluster (351 Da, Ligand)
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae W303)
- Eukaryotic translation initiation factor 3 subunit i (38 kDa, Protein from Saccharomyces cerevisiae W303)
- Rps29a isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae W303)
- Sui3 isoform 1 (31 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
Yeast cytoplasmic pre-40S ribosome biogenesis complex with deubiquitinating enzyme Otu2
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8cbj
Q-score: 0.263
Ikeuchi K, Ivic N, Buschauer R, Cheng J, Frohlich T, Matsuo Y, Berninghausen O, Inada T, Becker T, Beckmann R
Nat Commun (2023) 14 pp. 2730-2730 [ Pubmed: 37169754 DOI: doi:10.1038/s41467-023-38161-w ]
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
- Yeast cytoplasmic pre-40s ribosome biogenesis complex with deubiquitinating enzyme otu2 (Complex from Saccharomyces cerevisiae)
- Enp1 isoform 1 (55 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s4-b (29 kDa, Protein from Saccharomyces cerevisiae W303)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s3 (26 kDa, Protein from Saccharomyces cerevisiae W303)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae W303)
- Pre-rrna-processing protein pno1 (30 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae)
- Rps15 isoform 1 (16 kDa, Protein from Saccharomyces cerevisiae W303)
- 20s-pre-rrna d-site endonuclease nob1 (51 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae W303)
- 20s pre-ribosomal rna (647 kDa, RNA from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Rrna adenine n(6)-methyltransferase (36 kDa, Protein from Saccharomyces cerevisiae W303)
- Otu domain-containing protein 2 (36 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae W303)
- Tsr1 isoform 1 (90 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s25-a (12 kDa, Protein from Saccharomyces cerevisiae W303)
- Non-specific serine/threonine protein kinase (49 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s6-b (27 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae W303)
- Rps29a isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae W303)
- Ltv1 isoform 1 (53 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae W303)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae W303)
Resolution: 2.29 Å
EM Method: Single-particle
Fitted PDBs: 7z20
Q-score: 0.64
Ahn M, Wlodarski T, Mitropoulou A, Chan SHS, Sidhu H, Plessa E, Becker TA, Budisa N, Waudby CA, Beckmann R, Cassaignau AME, Cabrita LD, Christodoulou J
Nat Commun (2022) 13 pp. 4243-4243 [ DOI: doi:10.1038/s41467-022-31906-z Pubmed: 35869078 ]
- 23s rrna (941 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 5s rrna (38 kDa, RNA from Escherichia coli)
- Ribosomal nascent chain of fln5 (Complex from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- Gelation factor (16 kDa, Protein from Dictyostelium discoideum)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Pro-trna (24 kDa, RNA from Escherichia coli)
70S E. coli ribosome with an extended uL23 loop from Candidatus marinimicrobia
Resolution: 2.56 Å
EM Method: Single-particle
Fitted PDBs: 7zod
Q-score: 0.576
Ahn M, Wlodarski T, Mitropoulou A, Chan SHS, Sidhu H, Plessa E, Becker TA, Budisa N, Waudby CA, Beckmann R, Cassaignau AME, Cabrita LD, Christodoulou J
Nat Commun (2022) 13 pp. 4243-4243 [ DOI: doi:10.1038/s41467-022-31906-z Pubmed: 35869078 ]
- 23s rrna (941 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 5s rrna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- L23i empty ribosome (Complex from Escherichia coli 'BL21-Gold(DE3)pLysS AG')
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Pro-trna (24 kDa, RNA from Escherichia coli)