Cryo-EM structure of the 1:1 Orc1 BAH domain in complex with nucleosome
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7e9c
Q-score: 0.311
Jiang H, Yu C, Liu CP, Han X, Yu Z, Xu RM
To Be Published
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Origin recognition complex subunit 1 (Complex)
- Histone h2a.2 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h3 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Histone h4 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (Complex from Escherichia coli)
- Histone h2b.2 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Dna (147-mer) (45 kDa, DNA from Escherichia coli)
- Histone (Complex from Saccharomyces cerevisiae)
- Yeast orc1 bah domain in complex with 147-bp nucleosome (Complex from Saccharomyces cerevisiae)
- Origin recognition complex subunit 1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
The acetogenin-bound complex I of Mus musculus resolved to 3.4 angstroms
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7psa
Q-score: 0.516
Grba DN, Blaza JN, Bridges HR, Agip AA, Yin Z, Murai M, Miyoshi H, Hirst J
J Biol Chem (2022) 298 pp. 101602-101602 [ Pubmed: 35063503 DOI: doi:10.1016/j.jbc.2022.101602 ]
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from house mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (8 kDa, Protein from Mouse)
- Cardiolipin (1 kDa, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (50 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial (24 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 2 (38 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (16 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 4 (15 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from Mouse)
- ~{s}-[2-[3-[[(2~{r})-3,3-dimethyl-2-oxidanyl-4-phosphonooxy-butanoyl]amino]propanoylamino]ethyl] (3~{s})-3-oxidanyltetradecanethioate (584 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 4 (51 kDa, Protein from house mouse)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 5, mitochondrial (21 kDa, Protein from Mouse)
- Flavin mononucleotide (456 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (15 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 3 (11 kDa, Protein from Mouse)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from house mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (52 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (10 kDa, Protein from Mouse)
- Acyl carrier protein, mitochondrial (17 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 10 (21 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (21 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 11, mitochondrial (17 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial (30 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from Mouse)
- Zinc ion (65 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (19 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 11 (15 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from Mouse)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 subunit c1, mitochondrial (8 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 2, mitochondrial (11 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 3 (9 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from house mouse)
- Mitochondrial nadh:ubiquinone oxidoreductase from mus musculus (Complex from Mus musculus)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 1 (6 kDa, Protein from Mouse)
- (3~{s},5~{s})-5-methyl-3-[(13~{r})-13-oxidanyl-13-[(2~{r},5~{r})-5-[(2~{r},5~{r})-5-[(1~{r})-1-oxidanylundecyl]oxolan-2-yl]oxolan-2-yl]tridecyl]oxolan-2-one (608 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (13 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 9, mitochondrial (42 kDa, Protein from Mouse)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (24 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (79 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 9 (22 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (40 kDa, Protein from Mouse)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (18 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] flavoprotein 3, mitochondrial (11 kDa, Protein from Mouse)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (27 kDa, Protein from Mouse)
- Nadh-ubiquinone oxidoreductase chain 1 (36 kDa, Protein from house mouse)
Low resolution, multi-part 3D structure of the isolated yeast NPC
Akey CW, Singh D, Ouch C, Echeverria I, Nudelman I, Varberg JM, Yu Z, Fang F, Shi Y, Wang J, Salzberg D, Song K, Xu C, Gumbart JC, Suslov S, Unruh J, Jaspersen SL, Chait BT, Sali A, Fernandez-Martinez J, Ludtke SJ, Villa E, Rout MP
Cell (2021) [ Pubmed: 34982960 DOI: doi:10.1016/j.cell.2021.12.015 ]
- Central transporter (Complex from Saccharomyces cerevisiae)
- Double nuclear outer ring (Complex from Saccharomyces cerevisiae)
- 3d map of yeast npc (52 MDa, Complex)
- Nup82 complex hook ring (Complex from Saccharomyces cerevisiae)
- Inner spoke ring of yeast npc (Complex from Saccharomyces cerevisiae)
Loundras EA, Streetley J, Herod MR, Thompson R, Harris M, Bhella D, Stonehouse NJ
Commun Biol (2022) 5 pp. 61-61 [ DOI: doi:10.1038/s42003-021-02989-z Pubmed: 35039618 ]
Loundras EA, Streetley J, Herod MR, Thompson R, Harris M, Bhella D, Stonehouse NJ
Commun Biol (2022) 5 pp. 61-61 [ DOI: doi:10.1038/s42003-021-02989-z Pubmed: 35039618 ]
Loundras EA, Streetley J, Herod MR, Thompson R, Harris M, Bhella D, Stonehouse NJ
Commun Biol (2022) 5 pp. 61-61 [ DOI: doi:10.1038/s42003-021-02989-z Pubmed: 35039618 ]
Loundras EA, Streetley J, Herod MR, Thompson R, Harris M, Bhella D, Stonehouse NJ
Commun Biol (2022) 5 pp. 61-61 [ DOI: doi:10.1038/s42003-021-02989-z Pubmed: 35039618 ]
Loundras EA, Streetley J, Herod MR, Thompson R, Harris M, Bhella D, Stonehouse NJ
Commun Biol (2022) 5 pp. 61-61 [ DOI: doi:10.1038/s42003-021-02989-z Pubmed: 35039618 ]
Loundras EA, Streetley J, Herod MR, Thompson R, Harris M, Bhella D, Stonehouse NJ
Commun Biol (2022) 5 pp. 61-61 [ DOI: doi:10.1038/s42003-021-02989-z Pubmed: 35039618 ]
Loundras EA, Streetley J, Herod MR, Thompson R, Harris M, Bhella D, Stonehouse NJ
Commun Biol (2022) 5 pp. 61-61 [ DOI: doi:10.1038/s42003-021-02989-z Pubmed: 35039618 ]