Structure of the FDX2-bound core ISC complex (proximal conformation)
Resolution: 2.26 Å
EM Method: Single-particle
Fitted PDBs: 8rmc
Q-score: 0.702
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the FDX2-bound core ISC complex (distal conformation)
Resolution: 2.52 Å
EM Method: Single-particle
Fitted PDBs: 8rmd
Q-score: 0.642
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Human fdx2-bound core isc complex (nfs1-isd11-acp1-iscu2-fdx2) (Complex from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
Structure of the core ISC complex under turnover conditions (frataxin-bound)
Resolution: 2.49 Å
EM Method: Single-particle
Fitted PDBs: 8rme
Q-score: 0.615
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Frataxin mature form (14 kDa, Protein from Homo sapiens)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in proximal conformation)
Resolution: 2.33 Å
EM Method: Single-particle
Fitted PDBs: 8rmf
Q-score: 0.633
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Structure of the core ISC complex under turnover conditions (FDX2-bound in distal conformation)
Resolution: 2.46 Å
EM Method: Single-particle
Fitted PDBs: 8rmg
Q-score: 0.598
Steinhilper R, Boss L, Freibert SA, Schulz V, Krapoth N, Kaltwasser S, Lill R, Murphy BJ
Nat Commun (2024) 15 pp. 10559-10559 [ DOI: doi:10.1038/s41467-024-54585-4 Pubmed: 39632806 ]
- Isoform 1 of iron-sulfur cluster assembly enzyme iscu (15 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Ferredoxin-2, mitochondrial (13 kDa, Protein from Homo sapiens)
- Isoform mitochondrial of cysteine desulfurase (45 kDa, Protein from Homo sapiens)
- Fe (ii) ion (55 Da, Ligand)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Lyr motif-containing protein 4 (13 kDa, Protein from Homo sapiens)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)
- Acyl carrier protein (8 kDa, Protein from Escherichia coli BL21(DE3))
- Human core isc complex (nfs1-isd11-acp1-iscu2-fxn/fdx2) under turnover conditions (Complex from Homo sapiens)
Cryo-EM Structure of the 3HO-HCAR3-Gi complex
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 8jef
Q-score: 0.517
Ye F, Pan X, Zhang Z, Xiang X, Li X, Zhang B, Ning P, Liu A, Wang Q, Gong K, Li J, Zhu L, Qian C, Chen G, Du Y
Cell Rep (2024) 43 pp. 114895-114895 [ DOI: doi:10.1016/j.celrep.2024.114895 Pubmed: 39427321 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- (3s)-3-hydroxyoctanoic acid (160 Da, Ligand)
- Cryo-em structure of the apo hcar3-gi complex (Complex from Homo sapiens)
- Hydroxycarboxylic acid receptor 3 (44 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (6 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
Cryo-EM structure of human 26S proteasomal RP subcomplex (Ea state) without any bound substrate.
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8jri
Q-score: 0.443
Draczkowski P, Hsu STD, Wang YS
To Be Published
- Proteasome subunit alpha type-7 (27 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Human 26s proteasome (Complex from Homo sapiens)
- Aaa+-atpase of 19s regulatory particle (rp) of human 26s proteasome. (Complex from Homo sapiens)
- 26s protease regulatory subunit 6b (47 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-2 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-4 (29 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 10b (44 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 19s regulatory particle (rp) of human 26s proteasome. (Complex from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 12 (52 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 11 (47 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 7 (37 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 8 (39 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 8 (45 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 13 (42 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 6a (49 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-6 (27 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 7 (48 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-5 (26 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 6 (45 kDa, Protein from Homo sapiens)
- 26s proteasome complex subunit dss1 (8 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 26s proteasome non-atpase regulatory subunit 4 (40 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-1 (29 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 4 (49 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 1 (105 kDa, Protein from Homo sapiens)
- Alpha subunits belonging to the 20s core particle (cp) of human 26s proteasome. (Complex from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 14 (34 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 3 (61 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 2 (100 kDa, Protein from Homo sapiens)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 8jrt
Q-score: 0.423
Draczkowski P, Hsu STD, Wang YS
To Be Published
- Proteasome subunit alpha type-7 (27 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Human 26s proteasome (Complex from Homo sapiens)
- 26s protease regulatory subunit 6b (47 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-2 (25 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-4 (29 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 10b (44 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 26s proteasome non-atpase regulatory subunit 12 (52 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 11 (47 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 8 (39 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 7 (37 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 8 (45 kDa, Protein from Homo sapiens)
- 19s regulatory particle (rp) of human 26s proteasome (Complex from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 13 (42 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 6a (49 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-6 (27 kDa, Protein from Homo sapiens)
- 26s protease regulatory subunit 7 (48 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-5 (26 kDa, Protein from Homo sapiens)
- Aaa+-atpase of 19s regulatory particle (rp) of human 26s proteasome (Complex from Homo sapiens)
- K11/k48-branched ubiquitin (ub) chain composed of three ub (Complex from Homo sapiens)
- Polyubiquitin-b (8 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 6 (45 kDa, Protein from Homo sapiens)
- 26s proteasome complex subunit dss1 (8 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-3 (28 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 26s proteasome non-atpase regulatory subunit 4 (40 kDa, Protein from Homo sapiens)
- Proteasome subunit alpha type-1 (29 kDa, Protein from Homo sapiens)
- Alpha subunits belonging to the 20s core particle (cp) of human 26s proteasome (Complex from Homo sapiens)
- 26s protease regulatory subunit 4 (49 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 1 (105 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 14 (34 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 3 (61 kDa, Protein from Homo sapiens)
- 26s proteasome non-atpase regulatory subunit 2 (100 kDa, Protein from Homo sapiens)
Leukotriene C4-bound Multidrug Resistance-associated Protein 2 (MRP2)
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 9c12
Q-score: 0.53
Koide E, Pietz HL, Beltran J, Chen J
Nat Commun (2025) 16 pp. 484-484 [ DOI: doi:10.1038/s41467-024-55810-w Pubmed: 39779684 ]
- Wild type mrp2 bound to its substrate, leukotriene c4 (Cell from Homo sapiens)
- (5~{s},6~{r},7~{e},9~{e},11~{z},14~{z})-6-[(2~{r})-2-[[(4~{s})-4-azanyl-5-oxidanyl-5-oxidanylidene-pentanoyl]amino]-3-( 2-hydroxy-2-oxoethylamino)-3-oxidanylidene-propyl]sulfanyl-5-oxidanyl-icosa-7,9,11,14-tetraenoic acid (625 Da, Ligand)
- Cholesterol (386 Da, Ligand)
- Unknown ligand (790 Da, Ligand)
- Atp-binding cassette sub-family c member 2 (174 kDa, Protein from Homo sapiens)
Cryo-EM structure of E. coli AmpG
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 9c3f
Q-score: 0.431
Sverak HE, Yaeger LN, Worrall LJ, Vacariu CM, Glenwright AJ, Vuckovic M, Al Azawi ZD, Lamers RP, Marko VA, Skorupski C, Soni AS, Tanner ME, Burrows LL, Strynadka NC
Nat Commun (2024) 15 pp. 9936-9936 [ DOI: doi:10.1038/s41467-024-54219-9 Pubmed: 39548104 ]
- Anhydromuropeptide permease,soluble cytochrome b562 (65 kDa, Protein from Escherichia coli)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- Ampg-bril-bag2 (Complex from Escherichia coli)
- 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (744 Da, Ligand)