Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (Composite Map)
Resolution: 2.36 Å
EM Method: Single-particle
Fitted PDBs: 9e71
Q-score: 0.614
Nissley AJ, Shulgina Y, Kivimae RW, Downing BE, Penev PI, Banfield JF, Nayak DD, Cate JHD
To Be Published
- 70s ribosome (Complex from Pyrobaculum calidifontis JCM 11548)
- 16s rrna (487 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el21 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- El42 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el40 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el20 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el13 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el14 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us4 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s26e (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- As35 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul3 (37 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Es28 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s30e (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Parep1 domain containing protein (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el8 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul18 (23 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Zinc ion (65 Da, Ligand)
- Large ribosomal subunit protein el24 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us5 (22 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es4 (26 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul4 (31 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Lsu ribosomal protein l38e (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es27 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us15 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Spermine (202 Da, Ligand)
- Large ribosomal subunit protein el37 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us10 (12 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul13 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us13 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us17 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us9 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us12 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us14 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el34 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Water (18 Da, Ligand)
- Small ribosomal subunit protein es8 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us19 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el19 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul22 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small zinc finger protein hvo-2753-like zinc-binding pocket domain-containing protein (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es6 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul30 (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es1 (24 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us3 (24 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us2 (23 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us7 (25 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us11 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 50s ribosomal protein l15e (22 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s25e (12 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul6 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 50s subunit (Complex from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es17 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul23 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul14 (15 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el43 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul2 (26 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el18 (13 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el31 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul24 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 23s rrna (984 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el30 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul15 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul29 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el32 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 30s subunit (Complex from Pyrobaculum calidifontis JCM 11548)
- Dj-1/pfpi domain-containing protein (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein ul16 (19 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es19 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 5s rrna (41 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el39 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es24 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
Nissley AJ, Shulgina Y, Kivimae RW, Downing BE, Penev PI, Banfield JF, Nayak DD, Cate JHD
To Be Published
Nissley AJ, Shulgina Y, Kivimae RW, Downing BE, Penev PI, Banfield JF, Nayak DD, Cate JHD
To Be Published
Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome in complex with Dri (Composite Map)
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 9e7f
Q-score: 0.603
Nissley AJ, Shulgina Y, Kivimae RW, Downing BE, Penev PI, Banfield JF, Nayak DD, Cate JHD
To Be Published
- 70s ribosome (Complex from Pyrobaculum calidifontis JCM 11548)
- 16s rrna (487 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el21 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- El42 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el40 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el20 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el13 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el14 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us4 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s26e (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- As35 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Putative signal-transduction protein with cbs domains (72 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul3 (37 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s30e (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Parep1 domain containing protein (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el8 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul18 (23 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Zinc ion (65 Da, Ligand)
- Large ribosomal subunit protein el24 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us5 (22 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es4 (26 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul4 (31 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Lsu ribosomal protein l38e (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es27 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us15 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Spermine (202 Da, Ligand)
- Large ribosomal subunit protein el37 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us10 (12 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul13 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us13 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us17 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es28 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us9 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us12 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us14 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el34 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Water (18 Da, Ligand)
- Small ribosomal subunit protein es8 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us19 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el19 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul22 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small zinc finger protein hvo-2753-like zinc-binding pocket domain-containing protein (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es6 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul30 (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es1 (24 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us3 (24 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us2 (23 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us7 (25 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us11 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 50s ribosomal protein l15e (22 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s25e (12 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul6 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 50s subunit (Complex from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es17 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul23 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul14 (15 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el43 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul2 (26 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el18 (13 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el31 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul24 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 23s rrna (984 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el30 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul15 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul29 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el32 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 30s subunit (Complex from Pyrobaculum calidifontis JCM 11548)
- Dj-1/pfpi domain-containing protein (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein ul16 (19 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es19 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 5s rrna (41 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el39 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es24 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
E. coli 70S ribosome with A-loop mutations U2554C and U2555C (Composite Map)
Nissley AJ, Penev PI, Watson ZL, Banfield JF, Cate JHD
Nucleic Acids Res (2023) 51 pp. 1880-1894 [ Pubmed: 36660825 DOI: doi:10.1093/nar/gkac1273 ]
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
- Spermidine (145 Da, Ligand)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- Trna-fmet (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 30s subunit (Complex from Escherichia coli)
- Methionine (149 Da, Ligand)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- Paromomycin (615 Da, Ligand)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- Spermine (202 Da, Ligand)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 16s rrna (499 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 50s subunit (Complex from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- Ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 3'-amino-3'-deoxyadenosine 5'-(dihydrogen phosphate) (346 Da, Ligand)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- E. coli 70s ribosome with a-loop mutations u2554c and u2555c (850 kDa, Complex from Escherichia coli)
- Water (18 Da, Ligand)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- 23s rrna (941 kDa, RNA from Escherichia coli)
- Mrna (8 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
- 5s rrna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
E. coli 70S ribosome with A-loop mutations U2554C and U2555C (50S Focus Refinement)
Nissley AJ, Penev PI, Watson ZL, Banfield JF, Cate JHD
Nucleic Acids Res (2023) 51 pp. 1880-1894 [ Pubmed: 36660825 DOI: doi:10.1093/nar/gkac1273 ]
- E. coli 70s ribosome with a-loop mutations u2554c and u2555c (850 kDa, Complex from Escherichia coli)
- 30s subunit (Complex from Escherichia coli)
- 50s subunit (Complex from Escherichia coli)