Electron Microscopy Data Bank

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Release date: Oct. 21, 2020
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6w6k
Q-score: 0.288

Jahagirdar D, Jha V, Basu K, Gomez-Blanco J, Vargas J, Ortega J

RNA (2020) 26 pp. 2017-2030 [ Pubmed: 32989043 DOI: doi:10.1261/rna.075846.120 ]

Sample:

30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
30s-activated-high-mg2+ (Complex from Escherichia coli K-12)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
16s rrna (499 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))

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EMD-21569

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30S-Inactivated-high-Mg2+ Class A

Release date: Oct. 21, 2020
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6w77
Q-score: 0.354

Jahagirdar D, Jha V, Basu K, Gomez-Blanco J, Vargas J, Ortega J

RNA (2020) 26 pp. 2017-2030 [ Pubmed: 32989043 DOI: doi:10.1261/rna.075846.120 ]

Sample:

30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
30s-activated-high-mg2+ (Complex from Escherichia coli K-12)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
Rna (1519-mer) (499 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))

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EMD-21570

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30S-Inactive-high-Mg2+ Class B

Release date: Oct. 21, 2020
Resolution: 4.5 Å
EM Method: Single-particle

Jahagirdar D, Jha V, Basu K, Gomez-Blanco J, Vargas J, Ortega J

RNA (2020) 26 pp. 2017-2030 [ Pubmed: 32989043 DOI: doi:10.1261/rna.075846.120 ]

Sample:

30s-inactive-high-mg2+ class b (Complex from Escherichia coli K-12)

EMD-21571

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30S-Inactive-high-Mg2+ + carbon layer

Release date: Oct. 21, 2020
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6w7m
Q-score: 0.263

Jahagirdar D, Jha V, Basu K, Gomez-Blanco J, Vargas J, Ortega J

RNA (2020) 26 pp. 2017-2030 [ Pubmed: 32989043 DOI: doi:10.1261/rna.075846.120 ]

Sample:

30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
30s-activated-high-mg2+ (Complex from Escherichia coli K-12)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
16s rrna (499 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))

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EMD-21572

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30S-Inactive-low-Mg2+ Class A

Release date: Oct. 21, 2020
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6w7n
Q-score: 0.362

Jahagirdar D, Jha V, Basu K, Gomez-Blanco J, Vargas J, Ortega J

RNA (2020) 26 pp. 2017-2030 [ Pubmed: 32989043 DOI: doi:10.1261/rna.075846.120 ]

Sample:

30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
30s-activated-high-mg2+ (Complex from Escherichia coli K-12)
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
16s rrna (499 kDa, RNA from Escherichia coli (strain K12))
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))

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EMD-21573

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30S-Inactive-low-Mg2+ Class B

Release date: Oct. 21, 2020
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6w7w
Q-score: 0.361

Jahagirdar D, Jha V, Basu K, Gomez-Blanco J, Vargas J, Ortega J

RNA (2020) 26 pp. 2017-2030 [ Pubmed: 32989043 DOI: doi:10.1261/rna.075846.120 ]

Sample:

30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
16s rrna (499 kDa, RNA from Escherichia coli (strain K12))
30s-inactive-low-mg2+ class b (Complex from Escherichia coli K-12)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))

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EMD-22690

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30S-Inactive-low-Mg2+ Carbon

Release date: Oct. 21, 2020
Resolution: 3.2 Å
EM Method: Single-particle

Jahagirdar D, Jha V, Basu K, Gomez-Blanco J, Vargas J, Ortega J

RNA (2020) 26 pp. 2017-2030 [ Pubmed: 32989043 DOI: doi:10.1261/rna.075846.120 ]

Sample:

30s-inactive-low-mg2+ carbon (Complex from Escherichia coli K-12)

EMD-0481

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Role of Era in Assembly and Homeostasis of the Ribosomal Small Subunit

Release date: June 26, 2019
Resolution: 4.8 Å
EM Method: Single-particle

Razi A, Davis JH, Hao Y, Jahagirdar D, Thurlow B, Basu K, Jain N, Gomez-Blanco J, Britton RA, Vargas J, Guarne A, Woodson SA, Williamson JR, Ortega J

Nucleic Acids Res (2019) 47 pp. 8301-8317 [ DOI: doi:10.1093/nar/gkz571 Pubmed: 31265110 ]

Sample:

30s assembly intermediate (class d) (900 kDa, Complex from Escherichia coli)

EMD-0482

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Role of Era in Assembly and Homeostasis of the Ribosomal Small Subunit

Release date: June 26, 2019
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6nqb
Q-score: 0.332

Razi A, Davis JH, Hao Y, Jahagirdar D, Thurlow B, Basu K, Jain N, Gomez-Blanco J, Britton RA, Vargas J, Guarne A, Woodson SA, Williamson JR, Ortega J

Nucleic Acids Res (2019) 47 pp. 8301-8317 [ DOI: doi:10.1093/nar/gkz571 Pubmed: 31265110 ]

Sample:

30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
16s ribosomal rna (499 kDa, RNA from Escherichia coli)
30s ribosomal protein s6 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
30s assembly intermediate (class p) (900 kDa, Complex from Escherichia coli)
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli)
30s ribosomal protein bs16 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (6 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (8 kDa, Protein from Escherichia coli H736)
30s ribosomal protein s15 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)

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EMD-0483

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Role of Era in Assembly and Homeostasis of the Ribosomal Small Subunit

Release date: June 26, 2019
Resolution: 3.9 Å
EM Method: Single-particle

Razi A, Davis JH, Hao Y, Jahagirdar D, Thurlow B, Basu K, Jain N, Gomez-Blanco J, Britton RA, Vargas J, Guarne A, Woodson SA, Williamson JR, Ortega J

Nucleic Acids Res (2019) 47 pp. 8301-8317 [ DOI: doi:10.1093/nar/gkz571 Pubmed: 31265110 ]

Sample:

Era-treated mature 30s subunit (900 kDa, Complex from Escherichia coli)

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