Cas12k-sgRNA-dsDNA-S15-TniQ-TnsC transposon recruitment complex
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8bd5
Q-score: 0.39
Schmitz M, Querques I, Oberli S, Chanez C, Jinek M
Cell (2022) 185 pp. 4999- [ DOI: doi:10.1016/j.cell.2022.11.009 Pubmed: 36435179 ]
- Shcas12k (79 kDa, Protein from Scytonema hofmannii)
- Tniq (homology model) (19 kDa, Protein from Scytonema hofmannii)
- Target dna bound cas12k-sgrna-s15-tniq-tnsc transposon recruitment complex (Complex from Scytonema hofmannii)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Dna target strand (14 kDa, DNA from synthetic construct)
- Sgrna (82 kDa, RNA from Scytonema hofmannii)
- Dna non-target strand (15 kDa, DNA from synthetic construct)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
V-K CAST Transpososome from Scytonema hofmanni, major configuration
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8ea3
Q-score: 0.356
Park JU, Tsai AW, Rizo AN, Truong VH, Wellner TX, Schargel RD, Kellogg EH
Nature (2023) 613 pp. 775-782 [ Pubmed: 36442503 DOI: doi:10.1038/s41586-022-05573-5 ]
- Re_r2 (6 kDa, DNA from synthetic construct)
- Re_f (23 kDa, DNA from synthetic construct)
- 12_tnsc shcast transpososome (Complex from Scytonema hofmannii)
- Sg_rna (85 kDa, RNA from synthetic construct)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Non-target_r (21 kDa, DNA from synthetic construct)
- Cas12k (73 kDa, Protein from Scytonema hofmannii)
- Le_r (15 kDa, DNA from synthetic construct)
- Tniq (19 kDa, Protein from Scytonema hofmannii)
- Tnsb (66 kDa, Protein from Scytonema hofmannii)
- Target_le (43 kDa, DNA from synthetic construct)
- Re_r1 (15 kDa, DNA from synthetic construct)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
V-K CAST Transpososome from Scytonema hofmanni, minor configuration
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8ea4
Q-score: 0.302
Park JU, Tsai AW, Rizo AN, Truong VH, Wellner TX, Schargel RD, Kellogg EH
Nature (2023) 613 pp. 775-782 [ Pubmed: 36442503 DOI: doi:10.1038/s41586-022-05573-5 ]
- Re_r2 (6 kDa, DNA from synthetic construct)
- Re_f (23 kDa, DNA from synthetic construct)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- Sg_rna (85 kDa, RNA from synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Sh_cast transpososome (Complex from [Scytonema hofmanni] UTEX 2349)
- Non-target_r (21 kDa, DNA from synthetic construct)
- Le_r (15 kDa, DNA from synthetic construct)
- Tniq (19 kDa, Protein from Scytonema hofmannii)
- Tnsc (31 kDa, Protein from Scytonema hofmannii)
- Tnsb (66 kDa, Protein from Scytonema hofmannii)
- Re_r1 (15 kDa, DNA from synthetic construct)
- Target-le (43 kDa, DNA from synthetic construct)
- Cas12k (73 kDa, Protein from Scytonema hofmannii)