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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

Services:

offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"30S" AND xref_links:"interpro" AND database:"EMDB" AND software_ft:"CTFFIND"

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EMD-25421

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Pre translocation intermediate stalled with viomycin and bound with EF-G in a GDP and Pi state (Structure III-vio)

Release date: Feb. 23, 2022
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7st7
Q-score: 0.417

Carbone CE, Loveland AB, Gamper Jr HB, Hou YM, Demo G, Korostelev AA

Nat Commun (2021) 12 pp. 7236-7236 [ DOI: doi:10.1038/s41467-021-27415-0 Pubmed: 34903725 ]

Sample:

50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
16s rrna (498 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
Elongation factor g (76 kDa, Protein from Escherichia coli K-12)
Phosphate ion (94 Da, Ligand)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
Guanosine-5'-diphosphate (443 Da, Ligand)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
5s rrna (38 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
Trna pro (24 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
Pre translocation intermediate stalled with viomycin and bound with ef-g in a gdp and pi state (structure iii-vio) (2 MDa, Complex)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
Trna fmet (24 kDa, RNA from Escherichia coli K-12)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
Viomycin (703 Da, Protein from Streptomyces californicus)
50s ribosomal protein l1 (23 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
23s rrna (941 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
Mrna (6 kDa, RNA from Escherichia coli K-12)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli K-12)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)

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EMD-3852

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Elongation factor G-ribosome complex captures in the absence of inhibitors.

Release date: Feb. 14, 2018
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5ot7
Q-score: 0.344

Mace K, Giudice E, Chat S, Gillet R

Nucleic Acids Res (2018) 46 pp. 3211-3217 [ Pubmed: 29408956 DOI: doi:10.1093/nar/gky081 ]

Sample:

70s ribosome (Complex from Thermus thermophilus HB8)
50s ribosomal protein l18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l25 (20 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
5s ribosomal rna (38 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s11 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s12 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s16 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s20 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l34 (5 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l2 (30 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Mrna (4 kDa, RNA from Escherichia coli)
30s ribosomal protein s13 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l4 (23 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l9 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
16s ribosomal rna (489 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Trna-phe (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l13 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l29 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l28 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l22 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Magnesium ion (24 Da, Ligand)
Guanosine-5'-diphosphate (443 Da, Ligand)
30s ribosomal protein s7 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l23 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l19 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s17 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l31 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Ribosomal protein ul11 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Ribosomal protein ul10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l1 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l20 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s19 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l5 (20 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Ribosomal protein bl12 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Elongation factor g (76 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l36 (4 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l33 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
23s ribosomal rna (943 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Zinc ion (65 Da, Ligand)
50s ribosomal protein l14 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l6 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Elongation factor g (Complex from Thermus thermophilus)
30s ribosomal protein s2 (27 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Mrna (Complex from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s18 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l16 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l32 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l30 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Trna phe (Complex from Escherichia coli)
30s ribosomal protein s5 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l15 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l17 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l27 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l35 (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
Ef-g bound to 70s ribosome (Complex)
30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l24 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
50s ribosomal protein l21 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))

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EMD-4077

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Structure of bacterial 30S-IF1-IF3-mRNA-tRNA translation pre-initiation complex(state-2B)

Release date: Oct. 5, 2016
Resolution: 4.45 Å
EM Method: Single-particle
Fitted PDBs: 5lmr
Q-score: 0.294

Hussain T, Llacer JL, Wimberly BT, Kieft JS, Ramakrishnan V

Cell (2016) 167 pp. 133-144.e13 [ Pubmed: 27662086 DOI: doi:10.1016/j.cell.2016.08.074 ]

Sample:

30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus HB8)
16s rrna (493 kDa, RNA from Thermus thermophilus HB8)
30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus HB8)
Translation initiation factor if-1 (8 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s17 (12 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s19 (10 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s7 (18 kDa, Protein from Thermus thermophilus HB8)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s2 (29 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s16 (10 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s12 (14 kDa, Protein from Thermus thermophilus HB8)
30s-if1-if3-mrna-trna pre-initiation complex (state-2b) (849 kDa, Complex)
Mrna (13 kDa, RNA from Thermus thermophilus HB8)
30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus HB8)
Translation initiation factor if-3 (19 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s3 (26 kDa, Protein from Thermus thermophilus HB8)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s20 (11 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s13 (14 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s11 (13 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein s18 (10 kDa, Protein from Thermus thermophilus HB8)
Trnai (24 kDa, RNA from Thermus thermophilus HB8)
30s ribosomal protein s5 (17 kDa, Protein from Thermus thermophilus HB8)
30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus HB8)

[Show more]

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EMDB [3]

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