Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome (Composite Map)
Resolution: 2.36 Å
EM Method: Single-particle
Fitted PDBs: 9e71
Q-score: 0.614
Nissley AJ, Shulgina Y, Kivimae RW, Downing BE, Penev PI, Banfield JF, Nayak DD, Cate JHD
To Be Published
- Small ribosomal subunit protein us8 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 30s subunit (Complex from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein ul23 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 23s rrna (984 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Lsu ribosomal protein l38e (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es4 (26 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- As35 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es24 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us12 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Spermine (202 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Water (18 Da, Ligand)
- 50s ribosomal protein l15e (22 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us5 (22 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es6 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es8 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el24 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el18 (13 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 5s rrna (41 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul6 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us9 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us10 (12 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Parep1 domain containing protein (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s26e (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el21 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul13 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 50s subunit (Complex from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us11 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el14 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul3 (37 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el39 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el40 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us13 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 70s ribosome (Complex from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el8 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el13 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul29 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us19 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul22 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul15 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us4 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us7 (25 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el30 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us15 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Es28 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el32 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul14 (15 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el31 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul30 (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el20 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 16s rrna (487 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el34 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- El42 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el37 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el43 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es1 (24 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul2 (26 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us14 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es17 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul24 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us17 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small zinc finger protein hvo-2753-like zinc-binding pocket domain-containing protein (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul4 (31 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul16 (19 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul18 (23 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Dj-1/pfpi domain-containing protein (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us2 (23 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s30e (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us3 (24 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es27 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es19 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s25e (12 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el19 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
Nissley AJ, Shulgina Y, Kivimae RW, Downing BE, Penev PI, Banfield JF, Nayak DD, Cate JHD
To Be Published
Nissley AJ, Shulgina Y, Kivimae RW, Downing BE, Penev PI, Banfield JF, Nayak DD, Cate JHD
To Be Published
Cryo-EM structure of the Pyrobaculum calidifontis 70S ribosome in complex with Dri (Composite Map)
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 9e7f
Q-score: 0.603
Nissley AJ, Shulgina Y, Kivimae RW, Downing BE, Penev PI, Banfield JF, Nayak DD, Cate JHD
To Be Published
- Large ribosomal subunit protein ul2 (26 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul6 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us2 (23 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el20 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es8 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us15 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el18 (13 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Lsu ribosomal protein l38e (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us3 (24 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 50s ribosomal protein l15e (22 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul30 (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 23s rrna (984 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us11 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul22 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s26e (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us4 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 70s ribosome (Complex from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el21 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el34 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es24 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es6 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul14 (15 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us5 (22 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el8 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el13 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es1 (24 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul15 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us14 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul16 (19 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es4 (26 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es28 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul23 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el31 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul18 (23 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Putative signal-transduction protein with cbs domains (72 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el32 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Parep1 domain containing protein (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Water (18 Da, Ligand)
- 16s rrna (487 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us7 (25 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul4 (31 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Small ribosomal subunit protein us9 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 5s rrna (41 kDa, RNA from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us17 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us19 (18 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small zinc finger protein hvo-2753-like zinc-binding pocket domain-containing protein (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul29 (9 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el39 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es27 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us13 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el24 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul3 (37 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el30 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es17 (8 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein es19 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s25e (12 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- As35 (7 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el19 (17 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el40 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us10 (12 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 50s subunit (Complex from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul24 (14 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el37 (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Dj-1/pfpi domain-containing protein (20 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Small ribosomal subunit protein us12 (16 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein el14 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- El42 (10 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Large ribosomal subunit protein ul13 (21 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- Spermine (202 Da, Ligand)
- Large ribosomal subunit protein el43 (11 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
- 30s subunit (Complex from Pyrobaculum calidifontis JCM 11548)
- Ssu ribosomal protein s30e (6 kDa, Protein from Pyrobaculum calidifontis JCM 11548)
Structure of the 30S-IF3 complex from Escherichia coli
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 8jsg
Q-score: 0.265
Proteins (2025) 93 pp. 279-286 [ Pubmed: 38148682 DOI: doi:10.1002/prot.26655 ]
- Small ribosomal subunit protein us7 (16 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- Small ribosomal subunit protein us5 (16 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us13 (12 kDa, Protein from Escherichia coli)
- 30s subunit of ribosome (Cellular component)
- Small ribosomal subunit protein us2 (25 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us14 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs20 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us19 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us9 (14 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs21 (6 kDa, Protein from Escherichia coli)
- Initiation factor 3 (Cellular component from Escherichia coli)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- Translation initiation factor if-3 (20 kDa, Protein from Escherichia coli)
- Complex of the small ribosomal subunit (30s) and translation initiation factor 3 (if3) in escherichia coli (850 kDa, Complex from Escherichia coli)
- Small ribosomal subunit protein us3 (23 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs6, non-modified isoform (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us10 (11 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein bs18 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Structure of the 30S-body-IF3 complex from Escherichia coli
Resolution: 4.4 Å
EM Method: Single-particle
Fitted PDBs: 8jsh
Q-score: 0.294
Proteins (2025) 93 pp. 279-286 [ Pubmed: 38148682 DOI: doi:10.1002/prot.26655 ]
- Complex of the small ribosomal subunit (30s) body and translation initiation factor 3 (if3) in escherichia coli (Complex from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- Translation initiation factor if-3 (20 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (498 kDa, RNA from Escherichia coli)
- Initiation factor 3 (Cellular component from Escherichia coli)
- 30s ribosomal protein s6, fully modified isoform (15 kDa, Protein from Escherichia coli)
- Body of the 30s subunit of ribosome (Cellular component)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)