Structure of a yeast ABCE1-bound 48S initiation complex
Resolution: 6.2 Å
EM Method: Single-particle
Fitted PDBs: 6zu9
Q-score: 0.22
Kratzat H, Mackens-Kiani T, Ameismeier M, Potocnjak M, Cheng J, Dacheux E, Namane A, Berninghausen O, Herzog F, Fromont-Racine M, Becker T, Beckmann R
EMBO J (2021) 40 pp. e105179-e105179 [ Pubmed: 33289941 DOI: doi:10.15252/embj.2020105179 ]
- 40s ribosomal protein s3 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s25-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- Mrna (2 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 3 subunit c (93 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rps5p (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s26-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 1a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 3 subunit g (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Trna (24 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Eukaryotic translation initiation factor 5 (45 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s10-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 18s ribosomal rna (579 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 40s ribosomal protein s15 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Abce1-bound 48s initiation complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 3 subunit a (110 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Translation initiation factor rli1 (68 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 3 subunit i (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 3 subunit b (88 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s28-b (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Structure of a human ABCE1-bound 43S pre-initiation complex - State III
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7a09
Q-score: 0.399
Kratzat H, Mackens-Kiani T, Ameismeier M, Potocnjak M, Cheng J, Dacheux E, Namane A, Berninghausen O, Herzog F, Fromont-Racine M, Becker T, Beckmann R
EMBO J (2021) 40 pp. e105179-e105179 [ Pubmed: 33289941 DOI: doi:10.15252/embj.2020105179 ]
- 40s ribosomal protein s12 (14 kDa, Protein from Human)
- 40s ribosomal protein s28 (7 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 18s ribosomal rna (555 kDa, RNA from Human)
- Eukaryotic translation initiation factor 1a, x-chromosomal (12 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein sa (32 kDa, Protein from Human)
- 40s ribosomal protein s5 (22 kDa, Protein from Human)
- 40s ribosomal protein s15a (14 kDa, Protein from Human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Methionine (149 Da, Ligand)
- 40s ribosomal protein s19 (16 kDa, Protein from Human)
- 40s ribosomal protein s15 (17 kDa, Protein from Human)
- 40s ribosomal protein s4, x isoform (29 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s8 (24 kDa, Protein from Human)
- 40s ribosomal protein s9 (22 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit a (166 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit f (37 kDa, Protein from Human)
- Rna recognition motif (5 kDa, Protein from Human)
- 40s ribosomal protein s29 (6 kDa, Protein from Human)
- 40s ribosomal protein s25 (13 kDa, Protein from Human)
- 40s ribosomal protein s10 (18 kDa, Protein from Human)
- Eukaryotic translation initiation factor 2 subunit 3 (51 kDa, Protein from Human)
- Eukaryotic translation initiation factor 2 subunit 1 (36 kDa, Protein from Human)
- Receptor of activated protein c kinase 1 (35 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit e (52 kDa, Protein from Human)
- 40s ribosomal protein s3 (26 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit h (39 kDa, Protein from Human)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Trna (24 kDa, RNA from Human)
- Abce1-bound 43s pre-initiation complex (Complex from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit b (92 kDa, Protein from Human)
- Iron/sulfur cluster (351 Da, Ligand)
- 40s ribosomal protein s26 (13 kDa, Protein from Human)
- 60s ribosomal protein l41 (3 kDa, Protein from Human)
- 40s ribosomal protein s6 (28 kDa, Protein from Human)
- 40s ribosomal protein s16 (16 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit c,eukaryotic translation initiation factor 3 subunit c,eukaryotic translation initiation factor 3 subunit c (106 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit i (36 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit l (66 kDa, Protein from Human)
- Ubiquitin-40s ribosomal protein s27a (18 kDa, Protein from Human)
- 40s ribosomal protein s18 (17 kDa, Protein from Human)
- 40s ribosomal protein s27 (9 kDa, Protein from Human)
- 40s ribosomal protein s3a (30 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit k (25 kDa, Protein from Human)
- 40s ribosomal protein s17 (15 kDa, Protein from Human)
- 40s ribosomal protein s30 (6 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit m (42 kDa, Protein from Human)
- 40s ribosomal protein s23 (15 kDa, Protein from Human)
- 40s ribosomal protein s20 (13 kDa, Protein from Human)
- 40s ribosomal protein s24 (15 kDa, Protein from Human)
- 40s ribosomal protein s7 (22 kDa, Protein from Human)
- 40s ribosomal protein s13 (17 kDa, Protein from Human)
- Eukaryotic translation initiation factor 1 (12 kDa, Protein from Human)
- 40s ribosomal protein s11 (18 kDa, Protein from Human)
- Eukaryotic translation initiation factor 2 subunit 2 (38 kDa, Protein from Human)
- Eukaryotic translation initiation factor 3 subunit d (64 kDa, Protein from Human)
- Atp-binding cassette sub-family e member 1 (67 kDa, Protein from Human)
- 40s ribosomal protein s21 (9 kDa, Protein from Human)
- 40s ribosomal protein s14 (16 kDa, Protein from Human)
- 40s ribosomal protein s2 (31 kDa, Protein from Human)
Structure of a crosslinked yeast ABCE1-bound 43S pre-initiation complex
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7a1g
Q-score: 0.547
Kratzat H, Mackens-Kiani T, Ameismeier M, Potocnjak M, Cheng J, Dacheux E, Namane A, Berninghausen O, Herzog F, Fromont-Racine M, Becker T, Beckmann R
EMBO J (2021) 40 pp. e105179-e105179 [ DOI: doi:10.15252/embj.2020105179 Pubmed: 33289941 ]
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Eukaryotic translation initiation factor 3 subunit j (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s6-a (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s20 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s15 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Translation initiation factor rli1 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s30-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s10-a (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s12 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 18s ribosomal rna (570 kDa, RNA from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s14-b (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s11-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Abce1-bound 43s pic (Complex from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s13 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s3 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s5 (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s25-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Ubiquitin-40s ribosomal protein s31 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s26-b (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s17-b (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s18-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s2 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s9-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s23-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s0-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s1-a (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Structure of a yeast ABCE1-bound 43S pre-initiation complex
Resolution: 5.3 Å
EM Method: Single-particle
Fitted PDBs: 6zce
Q-score: 0.231
Kratzat H, Mackens-Kiani T, Ameismeier M, Potocnjak M, Cheng J, Dacheux E, Namane A, Berninghausen O, Herzog F, Fromont-Racine M, Becker T, Beckmann R
EMBO J (2021) 40 pp. e105179-e105179 [ Pubmed: 33289941 DOI: doi:10.15252/embj.2020105179 ]
- 40s ribosomal protein s0-a (28 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s17-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Abce1-bound 43s initiation complex (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Eukaryotic translation initiation factor 3 subunit i (38 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s12 (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s3 (26 kDa, Protein from Baker's yeast)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Translation initiation factor rli1 (68 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s15 (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit j (29 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s2 (27 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s18-a (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s19-a (15 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit c (93 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s26-a (13 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 18s ribosomal rna (1719-mer) (579 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Iron/sulfur cluster (351 Da, Ligand)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit g (30 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s20 (13 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s25-a (12 kDa, Protein from Baker's yeast)
- Rna recognition motif (unknown) (5 kDa, Protein from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor eif-1 (12 kDa, Protein from Baker's yeast)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit a (110 kDa, Protein from Baker's yeast)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s28-b (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s29-a (6 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 3 subunit b (88 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 1a (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s10-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s21-a (9 kDa, Protein from Baker's yeast)
Structure of a human ABCE1-bound 43S pre-initiation complex - State II
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 6zvj
Q-score: 0.365
Kratzat H, Mackens-Kiani T, Ameismeier M, Potocnjak M, Cheng J, Dacheux E, Namane A, Berninghausen O, Herzog F, Fromont-Racine M, Becker T, Beckmann R
EMBO J (2021) 40 pp. e105179-e105179 [ Pubmed: 33289941 DOI: doi:10.15252/embj.2020105179 ]
- Ubiquitin-40s ribosomal protein s27a (7 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit c (86 kDa, Protein from Homo sapiens)
- 40s ribosomal protein sa (24 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit f (29 kDa, Protein from Homo sapiens)
- 18s ribosomal rna (555 kDa, RNA from Homo sapiens)
- 40s ribosomal protein s15a (14 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s21 (8 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s27 (9 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit b (65 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit a (85 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit j (29 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s16 (15 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s4, x isoform (28 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- 40s ribosomal protein s6 (25 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s2 (23 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s8 (23 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s28 (6 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit m (41 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s24 (14 kDa, Protein from Homo sapiens)
- Rna recognition motif (unknown) (5 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s11 (16 kDa, Protein from Homo sapiens)
- Atp-binding cassette sub-family e member 1 (66 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s3a (24 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s25 (7 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s23 (15 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s10 (11 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s7 (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l41 (3 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s9 (21 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s20 (11 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s5 (21 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 1 (10 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 40s ribosomal protein s12 (12 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit h (36 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s13 (17 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s14 (13 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s17 (15 kDa, Protein from Homo sapiens)
- Receptor of activated protein c kinase 1 (34 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- 40s ribosomal protein s30 (5 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s18 (16 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s29 (6 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s26 (11 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit d (61 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit e (50 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit i (35 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 3 subunit k (24 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s3 (24 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s15 (14 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s19 (15 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Eukaryotic translation initiation factor 3 subunit l (44 kDa, Protein from Homo sapiens)
- Abce1-bound 43s initiation complex (Complex from Homo sapiens)
Cryo-EM structure of a native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5mc6
Q-score: 0.362
Schmidt C, Kowalinski E, Shanmuganathan V, Defenouillere Q, Braunger K, Heuer A, Pech M, Namane A, Berninghausen O, Fromont-Racine M, Jacquier A, Conti E, Becker T, Beckmann R
Science (2016) 354 pp. 1431-1433 [ DOI: doi:10.1126/science.aaf7520 Pubmed: 27980209 ]
- 60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s3 (26 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l25 (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l13-a (22 kDa, Protein from Baker's yeast)
- 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l11-a (19 kDa, Protein from Baker's yeast)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 5a-1 (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- Ul1 (17 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l10 (25 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l43-a (10 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s10-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l7-a (27 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l42-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s20 (13 kDa, Protein from Baker's yeast)
- Native ribosome-ski2-ski3-ski8 complex from s. cerevisiae (3 MDa, Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s2 (27 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- Antiviral helicase ski2 (146 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
- 18s ribosomal rna (579 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l41-a (3 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s26-a (13 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- 5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s12 (15 kDa, Protein from Baker's yeast)
- Superkiller protein 3 (163 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l5 (33 kDa, Protein from Baker's yeast)
- A-site trna (24 kDa, RNA from Baker's yeast)
- P-site trna (24 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s15 (16 kDa, Protein from Baker's yeast)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s17-b (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l6-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l34-a (13 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s28-b (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s29-a (6 kDa, Protein from Baker's yeast)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l30 (11 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s19-a (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
- Mrna (10 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s25-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l2-a (27 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l14-a (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l22-a (13 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l24-a (17 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l29 (6 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s0-a (28 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l8-a (28 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
- 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s18-a (17 kDa, Protein from Baker's yeast)
- Antiviral protein ski8 (44 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)