Cryo-EM structure of 90S small subunit preribosomes in transition states (State A)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 6lqp
Q-score: 0.471
Du Y, An W, Zhu X, Sun Q, Qi J, Ye K
Science (2020) 369 pp. 1477-1481 [ DOI: doi:10.1126/science.aba9690 Pubmed: 32943522 ]
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 22 (140 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s22-b (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 90s pre-ribosome (dhr1-depleted, state a) (5 MDa, Complex from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosome biogenesis protein utp30 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna cytidine acetyltransferase (119 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 6 (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pno1 (36 kDa, Protein from Saccharomyces cerevisiae S288C)
- U3 small nucleolar rna-associated protein mpp10 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleolar protein 58 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 11 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein sof1 (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleolar complex protein 4 (63 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 10 (200 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleolar complex protein 14 (94 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosomal rna-processing protein 7 (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 18 (66 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Bud site selection protein 21 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosome biogenesis protein bms1 (135 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 20 (287 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein faf1 (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 snorna (106 kDa, RNA from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 18s pre-rrna (582 kDa, RNA from Saccharomyces cerevisiae S288C)
- U3 small nucleolar rna-associated protein 7 (62 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 13 (91 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Regulator of rdna transcription protein 14 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 21 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Essential nuclear protein 1 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 14 (103 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Net1-associated nuclear protein 1 (101 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- U3 small nucleolar rna-associated protein 9 (65 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosomal rna small subunit methyltransferase nep1 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Krr1 small subunit processome component (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 4 (87 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 15 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rrna-processing protein fcf2 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleolar protein 56 (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Periodic tryptophan protein 2 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rrna-processing protein fcf1 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosome biogenesis protein enp2 (81 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 8 (80 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 5 (72 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 12 (106 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rrna 2'-o-methyltransferase fibrillarin (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 5' ets (225 kDa, RNA from Saccharomyces cerevisiae S288C)
- Ribosomal rna-processing protein 9 (65 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar ribonucleoprotein protein lcp5 (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Unassigned helices (29 kDa, Protein from Saccharomyces cerevisiae S288C)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein bfr2 (61 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Something about silencing protein 10 (70 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Cryo-EM structure of 90S small subunit preribosomes in transition states (State A1)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6lqu
Q-score: 0.269
Du Y, An W, Zhu X, Sun Q, Qi J, Ye K
Science (2020) 369 pp. 1477-1481 [ DOI: doi:10.1126/science.aba9690 Pubmed: 32943522 ]
- U3 small nucleolar rna-associated protein 5 (72 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rrna biogenesis protein rrp5 (193 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 20 (287 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosome biogenesis protein enp2 (81 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Magnesium ion (24 Da, Ligand)
- U3 small nucleolar rna-associated protein 22 (140 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosome biogenesis protein bms1 (135 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna cytidine acetyltransferase (119 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rrna-processing protein fcf2 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein bfr2 (61 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 9 (65 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 12 (106 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosomal rna-processing protein 9 (65 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 4 (87 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 15 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 13 kda ribonucleoprotein-associated protein (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleolar protein 56 (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 18 (66 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 7 (62 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein mpp10 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rrna-processing protein fcf1 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosome biogenesis protein utp30 (31 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Bud site selection protein 21 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleolar complex protein 14 (94 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Unassigned helices (29 kDa, Protein from Saccharomyces cerevisiae S288C)
- U3 small nucleolar rna-associated protein 6 (52 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Net1-associated nuclear protein 1 (101 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 11 (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rrna 2'-o-methyltransferase fibrillarin (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleolar protein 58 (57 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein faf1 (38 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 18s pre-rrna (582 kDa, RNA from Saccharomyces cerevisiae S288C)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 13 (91 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Krr1 small subunit processome component (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ribosomal rna-processing protein 7 (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 90s pre-ribosome (dhr1-depleted, state a1) (5 MDa, Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 8 (80 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Regulator of rdna transcription protein 14 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Essential nuclear protein 1 (55 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Periodic tryptophan protein 2 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 14 (103 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pno1 (36 kDa, Protein from Saccharomyces cerevisiae S288C)
- U3 small nucleolar rna-associated protein 21 (104 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s22-b (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ribosomal rna small subunit methyltransferase nep1 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Something about silencing protein 10 (70 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein sof1 (56 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar ribonucleoprotein protein lcp5 (40 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Nucleolar complex protein 4 (63 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 small nucleolar rna-associated protein 10 (200 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U3 snorna (106 kDa, RNA from Saccharomyces cerevisiae S288C)
- 5' ets (225 kDa, RNA from Saccharomyces cerevisiae S288C)