Cryo-EM structure of the yeast 80S ribosome with tigecycline, eEF2, Stm1 and eIF5A
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8k2d
Q-score: 0.423
Li X, Wang M, Denk T, Buschauer R, Li Y, Beckmann R, Cheng J
Nat Commun (2024) 15 pp. 5481-5481 [ Pubmed: 38942792 DOI: doi:10.1038/s41467-024-49797-7 ]
- Small ribosomal subunit protein us17a (17 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el18a (20 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el6a (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es17a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el20a (20 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul16 (25 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es28a (7 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el15a (24 kDa, Protein from Saccharomyces cerevisiae)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el8a (28 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-ribosomal protein el40a fusion protein (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul2a (27 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es26b (13 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us2a (28 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-ribosomal protein es31 fusion protein (17 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el19a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Tigecycline (587 Da, Ligand)
- Large ribosomal subunit protein ul5a (19 kDa, Protein from Saccharomyces cerevisiae)
- Yeast 80s ribosome (Complex from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el33a (12 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us10 (13 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Large ribosomal subunit protein el37a (9 kDa, Protein from Saccharomyces cerevisiae)
- 23s rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us7 (25 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el31a (12 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el32 (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el43a (10 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul11a (17 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el21a (18 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul6a (21 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul15 (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul14a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el29 (6 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 18s rrna (579 kDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el24a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul24a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul30a (27 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el42a (12 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul13a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el27a (15 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us19 (16 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul23 (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es21a (9 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul4a (39 kDa, Protein from Saccharomyces cerevisiae)
- Eukaryotic translation initiation factor 5a-1 (17 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el34a (13 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul29a (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us14a (6 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein el41a (3 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es19a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul1a (24 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el22a (13 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein rack1 (34 kDa, Protein from Saccharomyces cerevisiae)
- Suppressor protein stm1 (30 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el14a (15 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es12 (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el13a (22 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul18 (33 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us13a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul22a (20 kDa, Protein from Saccharomyces cerevisiae)
- Elongation factor 2 (93 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el36a (11 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es10a (12 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es25a (12 kDa, Protein from Saccharomyces cerevisiae)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Large ribosomal subunit protein el30 (11 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul10 (33 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el38 (8 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us9a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el39 (6 kDa, Protein from Saccharomyces cerevisiae)
Cryo-EM structure of the yeast 80S ribosome with tigecycline, Not5 and P-site tRNA
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8k82
Q-score: 0.453
Li X, Wang M, Denk T, Buschauer R, Li Y, Beckmann R, Cheng J
Nat Commun (2024) 15 pp. 5481-5481 [ Pubmed: 38942792 DOI: doi:10.1038/s41467-024-49797-7 ]
- Small ribosomal subunit protein us17a (17 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el18a (20 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el6a (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es17a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el20a (20 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul16 (25 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es28a (7 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el15a (24 kDa, Protein from Saccharomyces cerevisiae)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el8a (28 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-ribosomal protein el40a fusion protein (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul2a (27 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es26b (13 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us2a (28 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-ribosomal protein es31 fusion protein (17 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el19a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- Mrna (935 Da, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Tigecycline (587 Da, Ligand)
- Large ribosomal subunit protein ul5a (19 kDa, Protein from Saccharomyces cerevisiae)
- Yeast 80s ribosome (Complex from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el33a (12 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us10 (13 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Spermidine (145 Da, Ligand)
- Large ribosomal subunit protein el37a (9 kDa, Protein from Saccharomyces cerevisiae)
- 23s rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us7 (25 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el31a (12 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el32 (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el43a (10 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul11a (17 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el21a (18 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul6a (21 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul15 (16 kDa, Protein from Saccharomyces cerevisiae)
- Trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul14a (14 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el29 (6 kDa, Protein from Saccharomyces cerevisiae)
- General negative regulator of transcription subunit 5 (65 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 18s rrna (579 kDa, RNA from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el24a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul24a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul30a (27 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el42a (12 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul13a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el27a (15 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us19 (16 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul23 (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es21a (9 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul4a (39 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el34a (13 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul29a (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us14a (6 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein el41a (3 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us3 (26 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es19a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul1a (24 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el22a (13 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein rack1 (34 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el14a (15 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es12 (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el13a (22 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul18 (33 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us13a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul22a (20 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el36a (11 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es10a (12 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein es25a (12 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el30 (11 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein ul10 (33 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el38 (8 kDa, Protein from Saccharomyces cerevisiae)
- Small ribosomal subunit protein us9a (15 kDa, Protein from Saccharomyces cerevisiae)
- Large ribosomal subunit protein el39 (6 kDa, Protein from Saccharomyces cerevisiae)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 6zvi
Q-score: 0.511
Sinha NK, Ordureau A, Best K, Saba JA, Zinshteyn B, Sundaramoorthy E, Fulzele A, Garshott DM, Denk T, Thoms M, Paulo JA, Harper W, Bennett EJ, Beckmann R, Green R
eLife (2020) 9 [ DOI: doi:10.7554/eLife.58828 Pubmed: 32744497 ]
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Rps5p (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Rps12 isoform 1 (13 kDa, Protein from Saccharomyces cerevisiae)
- P/e-site trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- Mbf1 isoform 1 (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s18-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (24 kDa, Protein from Saccharomyces cerevisiae)
- Rps3 isoform 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s17-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s0-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Rps26b isoform 1 (11 kDa, Protein from Saccharomyces cerevisiae)
- Rps1a isoform 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Rps31 isoform 1 (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Mrna (10 kDa, RNA from Saccharomyces cerevisiae)
- Mbf1-ribosome complex (Complex from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Rps2 isoform 1 (23 kDa, Protein from Saccharomyces cerevisiae)
- A/p-site trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s11-a (16 kDa, Protein from Saccharomyces cerevisiae)
- Rps29a isoform 1 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-b (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s10-a (11 kDa, Protein from Saccharomyces cerevisiae)
- Rps20 isoform 1 (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- 18s rrna (566 kDa, RNA from Saccharomyces cerevisiae)
- Rps22a isoform 1 (14 kDa, Protein from Saccharomyces cerevisiae)
- Rps15 isoform 1 (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (5 kDa, Protein from Saccharomyces cerevisiae)
- Rps25a isoform 1 (7 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae)
- Rps28a isoform 1 (7 kDa, Protein from Saccharomyces cerevisiae)