Structure of LAT1-CD98hc-Fab170 in complex with JPH203, consensus map
Resolution: 3.83 Å
EM Method: Single-particle
Fitted PDBs: 8kdd
Q-score: 0.432
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Fab170 light chain (24 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lat1-cd98hc-fab170 (173 kDa, Complex)
- Fab170 (Complex from Mus musculus)
- Lat1-cd98hc (Complex from Homo sapiens)
- Nanvuranlat (472 Da, Ligand)
- Fab170 heavy chain (24 kDa, Protein from Mus musculus)
Structure of LAT1-CD98hc in complex with JPH203, focused on TMD
Resolution: 3.89 Å
EM Method: Single-particle
Fitted PDBs: 8kdf
Q-score: 0.458
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Nanvuranlat (472 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
Structure of LAT1-CD98hc-Fab170 in complex with BCH, consensus map
Resolution: 3.68 Å
EM Method: Single-particle
Fitted PDBs: 8kdg
Q-score: 0.424
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Fab170 light chain (24 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lat1-cd98hc-fab170 (173 kDa, Complex)
- Fab170 (Complex from Mus musculus)
- Lat1-cd98hc (Complex from Homo sapiens)
- Fab170 heavy chain (24 kDa, Protein from Mus musculus)
- (1~{s},2~{r},4~{r})-2-azanylbicyclo[2.2.1]heptane-2-carboxylic acid (155 Da, Ligand)
Structure of LAT1-CD98hc in complex with BCH, focused on TMD
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 8kdh
Q-score: 0.45
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
- (1~{s},2~{r},4~{r})-2-azanylbicyclo[2.2.1]heptane-2-carboxylic acid (155 Da, Ligand)
Structure of apo inward-open LAT1-CD98hc-Fab170 in nanodisc, consensus map
Resolution: 3.58 Å
EM Method: Single-particle
Fitted PDBs: 8kdi
Q-score: 0.43
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Fab170 light chain (24 kDa, Protein from Mus musculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Lat1-cd98hc (Complex from Homo sapiens)
- Fab170 (Complex from Mus musculus)
- Lat1-cd98hc-fab170 (Complex)
- Fab170 heavy chain (24 kDa, Protein from Mus musculus)
Structure of apo inward-open LAT1-CD98h in nanodisc, focused on TMD
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 8kdj
Q-score: 0.44
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (760 Da, Ligand)
Structure of LAT1-CD98hc in complex with L-Phe, focused on TMD
Resolution: 4.12 Å
EM Method: Single-particle
Fitted PDBs: 8kdn
Q-score: 0.413
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Phenylalanine (165 Da, Ligand)
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
Structure of LAT1-CD98hc in complex with melphalan, focused on TMD
Resolution: 4.12 Å
EM Method: Single-particle
Fitted PDBs: 8kdo
Q-score: 0.417
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- (2~{s})-2-azanyl-3-[4-[bis(2-chloroethyl)amino]phenyl]propanoic acid (305 Da, Ligand)
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
Structure of apo outward-open LAT1-CD98h in nanodisc, focused on TMD
Resolution: 4.12 Å
EM Method: Single-particle
Fitted PDBs: 8kdp
Q-score: 0.409
Lee Y, Jin C, Ohgaki R, Xu M, Ogasawara S, Warshamanage R, Yamashita K, Murshudov G, Nureki O, Murata T, Kanai Y
Nat Commun (2025) 16 pp. 1635-1635 [ DOI: doi:10.1038/s41467-025-56903-w Pubmed: 39952931 ]
- Lat1-cd98hc-fab170 (173 kDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)
LAT1-CD98hc complex bound to MEM-108 Fab
Resolution: 3.31 Å
EM Method: Single-particle
Fitted PDBs: 6jmq
Q-score: 0.487
Lee Y, Wiriyasermkul P, Jin C, Quan L, Ohgaki R, Okuda S, Kusakizako T, Nishizawa T, Oda K, Ishitani R, Yokoyama T, Nakane T, Shirouzu M, Endou H, Nagamori S, Kanai Y, Nureki O
Nat Struct Mol Biol (2019) 26 pp. 510-517 [ DOI: doi:10.1038/s41594-019-0237-7 Pubmed: 31160781 ]
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Antibody (23 kDa, Protein from Mus musculus)
- Antibody (22 kDa, Protein from Mus musculus)
- Lat1-cd98hc complex bound to mem-108 fab (175 MDa, Complex from Homo sapiens)
- 4f2 cell-surface antigen heavy chain (68 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)
- Large neutral amino acids transporter small subunit 1 (56 kDa, Protein from Homo sapiens)