Structure of a group II intron ribonucleoprotein in the pre-ligation (pre-2F) state
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8t2r
Q-score: 0.459
Nature (2023) 624 pp. 682-688 [ Pubmed: 37993708 DOI: doi:10.1038/s41586-023-06746-6 ]
- Ammonium ion (18 Da, Ligand)
- Calcium ion (40 Da, Ligand)
- 5'exon (2 kDa, RNA from [Eubacterium] rectale)
- Intron (208 kDa, RNA from [Eubacterium] rectale)
- Intron rnp complex in pre-2f (300 kDa, Complex from [Eubacterium] rectale)
- Group ii intron reverse transcriptase/maturase (49 kDa, Protein from [Eubacterium] rectale)
Human spliceosomal PM5 C* complex
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8c6j
Q-score: 0.353
Dybkov O, Preussner M, El Ayoubi L, Feng VY, Harnisch C, Merz K, Leupold P, Yudichev P, Agafonov DE, Will CL, Girard C, Dienemann C, Urlaub H, Kastner B, Heyd F, Luehrmann R
Sci Adv (2023) 9 pp. eadf1785- [ Pubmed: 36867703 DOI: doi:10.1126/sciadv.adf1785 ]
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor isy1 homolog (33 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor syf2 (28 kDa, Protein from Homo sapiens)
- Human spliceosomal c* complex assembled on pm5 pre-mrna (Complex from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor rbm22 (46 kDa, Protein from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- Prkr-interacting protein 1 (21 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 17 (65 kDa, Protein from Homo sapiens)
- Nitric oxide synthase-interacting protein (33 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor cwc22 homolog (57 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Pm5 intron (74 kDa, RNA from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Protein fam50a (40 kDa, Protein from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase-like 3 (18 kDa, Protein from Homo sapiens)
- Atp-dependent rna helicase dhx8 (139 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Splicing factor cactin (88 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Protein bud31 homolog (17 kDa, Protein from Homo sapiens)
- U5 snrna (36 kDa, RNA from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Sting er exit protein (25 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx41 (69 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Crooked neck-like protein 1 (83 kDa, Protein from Homo sapiens)
- Serine/arginine repetitive matrix protein 2 (300 kDa, Protein from Homo sapiens)
- Rna-binding protein 8a (19 kDa, Protein from Homo sapiens)
- Protein fam32a (13 kDa, Protein from Homo sapiens)
- Snw domain-containing protein 1 (61 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase-like 1 (18 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Splicing regulator sde2 (49 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Protein mago nashi homolog (17 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Eukaryotic initiation factor 4a-iii (46 kDa, Protein from Homo sapiens)
- Pleiotropic regulator 1 (57 kDa, Protein from Homo sapiens)
- Cell division cycle 5-like protein (92 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 19 (55 kDa, Protein from Homo sapiens)
- Intron-binding protein aquarius (171 kDa, Protein from Homo sapiens)
- Pm5 5' exon (78 kDa, RNA from Homo sapiens)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor slu7 (68 kDa, Protein from Homo sapiens)
- Splicing factor ess-2 homolog (52 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor spf27 (26 kDa, Protein from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase e (33 kDa, Protein from Homo sapiens)
- Splicing factor c9orf78 (33 kDa, Protein from Homo sapiens)
- Nf-kappa-b-activating protein (47 kDa, Protein from Homo sapiens)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Homo sapiens)
The Tet-S2 state of wild-type Tetrahymena group I intron with 30nt 3'/5'-exon
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 7xd5
Q-score: 0.504
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- The tet-s2 state molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (intron and 3'-exon) (129 kDa, RNA from Tetrahymena thermophila)
- Spermidine (145 Da, Ligand)
- Co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (Complex from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- The tet-s2 state molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (5'-exon) (1 kDa, RNA from Tetrahymena thermophila)
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 7xd6
Q-score: 0.476
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Spermidine (145 Da, Ligand)
- The tet-s2 state with a pseudoknotted 4-way junction molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (5'-exon) (8 kDa, RNA from Tetrahymena thermophila)
- Co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (Complex from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- The tet-s2 state with a pseudoknotted 4-way junction molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (intron and 3'-exon) (140 kDa, RNA from Tetrahymena thermophila)
The relaxed pre-Tet-S1 state of wild-type Tetrahymena group I intron with 6nt 3'/5'-exon
Resolution: 4.05 Å
EM Method: Single-particle
Fitted PDBs: 7xd3
Q-score: 0.335
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Co-transcriptional folded wild-type tetrahymena group i intron with 6nt 3'/5'-exon (Complex from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- The relaxed pre-tet-s1 state molecule of co-transcriptional folded wild-type tetrahymena group i intron with 6nt 3'/5'-exon (137 kDa, RNA from Tetrahymena thermophila)
The intermediate pre-Tet-S1 state of wild-type Tetrahymena group I intron with 6nt 3'/5'-exon
Resolution: 3.89 Å
EM Method: Single-particle
Fitted PDBs: 7xd4
Q-score: 0.32
The pre-Tet-C state of wild-type Tetrahymena group I intron with 30nt 3'/5'-exon
Resolution: 3.02 Å
EM Method: Single-particle
Fitted PDBs: 7xd7
Q-score: 0.473
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Spermidine (145 Da, Ligand)
- Co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (Complex from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- The pre-tet-c state molecule of co-transcriptional folded wild-type tetrahymena group i intron with 30nt 3'/5'-exon (130 kDa, RNA from Tetrahymena thermophila)
Resolution: 3.73 Å
EM Method: Single-particle
Fitted PDBs: 8hd6
Q-score: 0.319
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Co-transcriptional folded g264a mutant tetrahymena group i intron with 6nt 5'/3'-exon and 2-aminopurine nucleoside (Complex from Tetrahymena thermophila)
- The relaxed pre-tet-s1 state molecule of co-transcriptional folded g264a mutant tetrahymena group i intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside (135 kDa, RNA from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- Spermidine (145 Da, Ligand)
Resolution: 3.52 Å
EM Method: Single-particle
Fitted PDBs: 8hd7
Q-score: 0.364
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Magnesium ion (24 Da, Ligand)
- Co-transcriptional folded g264a mutated tetrahymena group i intron with 6-nt 3'/5'-exon and 2-aminopurine nucleoside (Complex from Tetrahymena thermophila)
- The intermediate pre-tet-s1 state molecule of co-transcriptional folded g264a mutant tetrahymena group i intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside (135 kDa, RNA from Tetrahymena thermophila)
- Spermidine (145 Da, Ligand)
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 8i7n
Q-score: 0.518
Luo B, Zhang C, Ling X, Mukherjee S, Jia G, Xie J, Jia X, Liu L, Baulin EF, Luo Y, Jiang L, Dong H, Wei X, Bujnicki JM, Su Z
Nat Catal (2023) [ DOI: doi:10.1038/s41929-023-00934-3 ]
- Spermidine (145 Da, Ligand)
- Co-transcriptional folded g264a mutated tetrahymena group i intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside (Complex from Tetrahymena thermophila)
- The tet-s1 state molecule of co-transcriptional folded g264a mutated tetrahymena group i intron with 6nt 3'/5'-exon and 2-aminopurine nucleoside (134 kDa, RNA from Tetrahymena thermophila)
- Magnesium ion (24 Da, Ligand)
- (2r,3r,4s,5r)-2-(2-azanylpurin-9-yl)-5-(hydroxymethyl)oxolane-3,4-diol (267 Da, Ligand)