Resolution: 9.1 Å
EM Method: Subtomogram averaging
Fitted PDBs: 6ftg
Q-score: 0.126
Braunger K, Pfeffer S, Shrimal S, Gilmore R, Berninghausen O, Mandon EC, Becker T, Forster F, Beckmann R
Science (2018) 360 pp. 215-219 [ DOI: doi:10.1126/science.aar7899 Pubmed: 29519914 ]
- 60s ribosomal protein l6 (28 kDa, Protein from Rabbit)
- Ribosomal protein l10 (predicted) (24 kDa, Protein from Rabbit)
- El32 (15 kDa, Protein from Rabbit)
- El29 (14 kDa, Protein from Rabbit)
- Rpn2 (6 kDa, Protein from Dog)
- Ribosomal protein l11 (19 kDa, Protein from Rabbit)
- Ul13 (23 kDa, Protein from Rabbit)
- 60s ribosomal protein l41 (3 kDa, Protein from Rabbit)
- El22 (11 kDa, Protein from Rabbit)
- Protein transport protein sec61 subunit beta (3 kDa, Protein from Dog)
- Zinc ion (65 Da, Ligand)
- El8 (27 kDa, Protein from Rabbit)
- El30 (10 kDa, Protein from Rabbit)
- El34 (12 kDa, Protein from Rabbit)
- Ost4 (3 kDa, Protein from Dog)
- Ul14 (21 kDa, Protein from Rabbit)
- Ribosomal protein l24 (7 kDa, Protein from Rabbit)
- 60s ribosomal protein l5 (34 kDa, Protein from Rabbit)
- Ul30 (26 kDa, Protein from Rabbit)
- Ul2 (26 kDa, Protein from Rabbit)
- Sec61 protein conducting channel (Complex from Canis lupus familiaris)
- 60s acidic ribosomal protein p0 (21 kDa, Protein from Rabbit)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Dog)
- Protein transport protein sec61 subunit alpha isoform 1 (50 kDa, Protein from Dog)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3a (80 kDa, Protein from Dog)
- 28s ribosomal rna (1 MDa, RNA from Rabbit)
- [(2~{s},3~{r},4~{r},5~{s},6~{r})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{z},6~{z},10~{z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid (651 Da, Ligand)
- Tmem258 (5 kDa, Protein from Dog)
- Ribosomal protein l4 (41 kDa, Protein from Rabbit)
- 80s ribosome from wt canine rough microsomal membranes bound to the endoplasmic reticulum protein translocon (Complex)
- El38 (8 kDa, Protein from Rabbit)
- 60s ribosomal protein l29 (8 kDa, Protein from Rabbit)
- Ribosomal protein l12 (17 kDa, Protein from Rabbit)
- 5s ribosomal rna (38 kDa, RNA from Rabbit)
- Ost48 (2 kDa, Protein from Dog)
- El13 (24 kDa, Protein from Rabbit)
- El28 (15 kDa, Protein from Rabbit)
- Mammalian oligosaccharyltransferase (Complex from Canis lupus familiaris)
- El39 (6 kDa, Protein from Rabbit)
- Ul6 (21 kDa, Protein from Rabbit)
- El21 (18 kDa, Protein from Rabbit)
- El19 (21 kDa, Protein from Rabbit)
- Ribosomal protein l15 (24 kDa, Protein from Rabbit)
- 60s ribosomal protein l27 (15 kDa, Protein from Rabbit)
- 60s ribosomal protein l36 (11 kDa, Protein from Rabbit)
- Ribosomal protein l14 (16 kDa, Protein from Rabbit)
- El40 (6 kDa, Protein from Rabbit)
- 5.8s ribosomal rna (50 kDa, RNA from Rabbit)
- El20 (20 kDa, Protein from Rabbit)
- El42 (12 kDa, Protein from Rabbit)
- Ribosomal protein l37 (10 kDa, Protein from Rabbit)
- El33 (12 kDa, Protein from Rabbit)
- Ul22 (17 kDa, Protein from Rabbit)
- Ul15 (16 kDa, Protein from Rabbit)
- El31 (12 kDa, Protein from Rabbit)
- 60s ribosomal subunit (Complex from Oryctolagus cuniculus)
- Ul14 (13 kDa, Protein from Rabbit)
- Dad1 (8 kDa, Protein from Dog)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1,rpn1 (14 kDa, Protein from Dog)
- Ribosomal protein l26 (15 kDa, Protein from Rabbit)
- Oligosaccharyltransferase complex subunit ostc (13 kDa, Protein from Dog)
- Ribosomal protein l37a (10 kDa, Protein from Rabbit)
- Ul3 (45 kDa, Protein from Rabbit)
- Magnesium ion (24 Da, Ligand)
- Ul23 (13 kDa, Protein from Rabbit)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6fti
Q-score: 0.207
Braunger K, Pfeffer S, Shrimal S, Gilmore R, Berninghausen O, Mandon EC, Becker T, Forster F, Beckmann R
Science (2018) 360 pp. 215-219 [ DOI: doi:10.1126/science.aar7899 Pubmed: 29519914 ]
- Oligosaccharyltransferase (Complex from Canis lupus familiaris)
- 60s ribosomal protein l6 (28 kDa, Protein from Rabbit)
- Ribosomal protein l10 (predicted) (24 kDa, Protein from Rabbit)
- El32 (15 kDa, Protein from Rabbit)
- Ribosomal protein l11 (19 kDa, Protein from Rabbit)
- Ul13 (23 kDa, Protein from Rabbit)
- Ul29 (14 kDa, Protein from Rabbit)
- 60s ribosomal protein l41 (3 kDa, Protein from Rabbit)
- P-site trna (24 kDa, RNA from Rabbit)
- El22 (11 kDa, Protein from Rabbit)
- Protein transport protein sec61 subunit beta (3 kDa, Protein from Dog)
- Zinc ion (65 Da, Ligand)
- El8 (27 kDa, Protein from Rabbit)
- El30 (10 kDa, Protein from Rabbit)
- El34 (12 kDa, Protein from Rabbit)
- Ul14 (21 kDa, Protein from Rabbit)
- Ribosomal protein l24 (7 kDa, Protein from Rabbit)
- Unidentified tm (2 kDa, Protein from Dog)
- 60s ribosomal protein l5 (34 kDa, Protein from Rabbit)
- Ul30 (26 kDa, Protein from Rabbit)
- Ul2 (26 kDa, Protein from Rabbit)
- Sec61 protein conducting channel (Complex from Canis lupus familiaris)
- 60s acidic ribosomal protein p0 (21 kDa, Protein from Rabbit)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Dog)
- Protein transport protein sec61 subunit alpha isoform 1 (50 kDa, Protein from Dog)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3a (80 kDa, Protein from Dog)
- [(2~{s},3~{r},4~{r},5~{s},6~{r})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{z},6~{z},10~{z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid (651 Da, Ligand)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1,rpn1 (12 kDa, Protein from Dog)
- 60s ribosomal subunit (Complex from Oryctolagus cuniculus)
- Ribosomal protein l4 (41 kDa, Protein from Rabbit)
- Dad1 (9 kDa, Protein from Dog)
- El38 (8 kDa, Protein from Rabbit)
- 60s ribosomal protein l29 (8 kDa, Protein from Rabbit)
- Ribosomal protein l12 (17 kDa, Protein from Rabbit)
- 5s ribosomal rna (38 kDa, RNA from Rabbit)
- El13 (24 kDa, Protein from Rabbit)
- El28 (15 kDa, Protein from Rabbit)
- Ost48 (2 kDa, Protein from Dog)
- El39 (6 kDa, Protein from Rabbit)
- Ul6 (21 kDa, Protein from Rabbit)
- El21 (18 kDa, Protein from Rabbit)
- El19 (21 kDa, Protein from Rabbit)
- Ribosomal protein l15 (24 kDa, Protein from Rabbit)
- 60s ribosomal protein l27 (15 kDa, Protein from Rabbit)
- 60s ribosomal protein l36 (11 kDa, Protein from Rabbit)
- Ribosomal protein l14 (16 kDa, Protein from Rabbit)
- El40 (6 kDa, Protein from Rabbit)
- 28s rrna (1 MDa, RNA from Rabbit)
- 5.8s ribosomal rna (50 kDa, RNA from Rabbit)
- El20 (20 kDa, Protein from Rabbit)
- El42 (12 kDa, Protein from Rabbit)
- Ribosomal protein l37 (10 kDa, Protein from Rabbit)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 4 (3 kDa, Protein from Dog)
- El33 (12 kDa, Protein from Rabbit)
- Ul22 (17 kDa, Protein from Rabbit)
- Ul15 (16 kDa, Protein from Rabbit)
- El31 (12 kDa, Protein from Rabbit)
- Ul14 (13 kDa, Protein from Rabbit)
- Rpn1 (6 kDa, Protein from Dog)
- Mammalian oligosaccharyltransferase in complex with sec61 and the 80s ribosome (Complex)
- Ribosomal protein l26 (15 kDa, Protein from Rabbit)
- Tmem258 (4 kDa, Protein from Dog)
- Oligosaccharyltransferase complex subunit ostc (13 kDa, Protein from Dog)
- Ribosomal protein l37a (10 kDa, Protein from Rabbit)
- Ul3 (45 kDa, Protein from Rabbit)
- Magnesium ion (24 Da, Ligand)
- Ul23 (13 kDa, Protein from Rabbit)
Resolution: 4.7 Å
EM Method: Single-particle
Fitted PDBs: 6ftj
Q-score: 0.174
Braunger K, Pfeffer S, Shrimal S, Gilmore R, Berninghausen O, Mandon EC, Becker T, Forster F, Beckmann R
Science (2018) 360 pp. 215-219 [ Pubmed: 29519914 DOI: doi:10.1126/science.aar7899 ]
- 28s rrna (1 MDa, RNA from Oryctolagus cuniculus)
- Mammalian oligosaccharyltransferase in complex with sec61 and the non-programmed 80s ribosome (Complex)
- 60s ribosomal protein l36 (11 kDa, Protein from Oryctolagus cuniculus)
- Protein transport protein sec61 subunit beta (3 kDa, Protein from Canis lupus familiaris)
- Protein transport protein sec61 subunit alpha isoform 1 (47 kDa, Protein from Canis lupus familiaris)
- 60s ribosomal protein l5 (34 kDa, Protein from Oryctolagus cuniculus)
- Rpn2 (6 kDa, Protein from Canis lupus familiaris)
- Ul2 (26 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l24 (7 kDa, Protein from Oryctolagus cuniculus)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Canis lupus familiaris)
- Zinc ion (65 Da, Ligand)
- El40 (6 kDa, Protein from Oryctolagus cuniculus)
- 60s acidic ribosomal protein p0 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l6 (28 kDa, Protein from Oryctolagus cuniculus)
- El20 (20 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l4 (41 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l15 (24 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l11 (19 kDa, Protein from Oryctolagus cuniculus)
- Dad1 (8 kDa, Protein from Canis lupus familiaris)
- 5.8s ribosomal rna (50 kDa, RNA from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- El33 (12 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l29 (8 kDa, Protein from Oryctolagus cuniculus)
- El31 (12 kDa, Protein from Oryctolagus cuniculus)
- El39 (6 kDa, Protein from Oryctolagus cuniculus)
- Ul6 (21 kDa, Protein from Oryctolagus cuniculus)
- El19 (21 kDa, Protein from Oryctolagus cuniculus)
- El28 (15 kDa, Protein from Oryctolagus cuniculus)
- Ost48 (2 kDa, Protein from Canis lupus familiaris)
- Ul15 (16 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal subunit (Complex from Oryctolagus cuniculus)
- El13 (24 kDa, Protein from Oryctolagus cuniculus)
- Ul8 (27 kDa, Protein from Oryctolagus cuniculus)
- Tmem258 (5 kDa, Protein from Canis lupus familiaris)
- Ul22 (17 kDa, Protein from Oryctolagus cuniculus)
- Ul13 (23 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l37a (10 kDa, Protein from Oryctolagus cuniculus)
- El34 (12 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l26 (15 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l14 (16 kDa, Protein from Oryctolagus cuniculus)
- Ul14 (21 kDa, Protein from Oryctolagus cuniculus)
- Ul14 (13 kDa, Protein from Oryctolagus cuniculus)
- Ul29 (14 kDa, Protein from Oryctolagus cuniculus)
- Ul23 (13 kDa, Protein from Oryctolagus cuniculus)
- Sec61 protein conducting channel (Complex from Canis lupus familiaris)
- Ul3 (45 kDa, Protein from Oryctolagus cuniculus)
- Oligosaccharyltransferase complex subunit ostc (13 kDa, Protein from Canis lupus familiaris)
- El30 (10 kDa, Protein from Oryctolagus cuniculus)
- El42 (12 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l37 (10 kDa, Protein from Oryctolagus cuniculus)
- El21 (18 kDa, Protein from Oryctolagus cuniculus)
- Ul30 (26 kDa, Protein from Oryctolagus cuniculus)
- Ost4 (3 kDa, Protein from Canis lupus familiaris)
- El38 (8 kDa, Protein from Oryctolagus cuniculus)
- [(2~{s},3~{r},4~{r},5~{s},6~{r})-3-acetamido-6-(hydroxymethyl)-4,5-bis(oxidanyl)oxan-2-yl]methyl-[oxidanyl-[(2~{z},6~{z},10~{z})-3,7,11,15-tetramethylhexadeca-2,6,10,14-tetraenoxy]phosphoryl]oxy-phosphinic acid (651 Da, Ligand)
- 5s ribosomal rna (38 kDa, RNA from Oryctolagus cuniculus)
- Oligosaccharyltransferase (Complex from Canis lupus familiaris)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit stt3a (79 kDa, Protein from Canis lupus familiaris)
- Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1,rpn1 (17 kDa, Protein from Canis lupus familiaris)
- Ribosomal protein l12 (17 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l22 (11 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l41 (3 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l10 (predicted) (24 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27 (15 kDa, Protein from Oryctolagus cuniculus)
- El32 (15 kDa, Protein from Oryctolagus cuniculus)