Stringent response control by a bifunctional RelA enzyme in the presence and absence of the ribosome
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 6htq
Q-score: 0.355
Pausch P, Abdelshahid M, Steinchen W, Schafer H, Gratani FL, Freibert SA, Wolz C, Turgay K, Wilson DN, Bange G
Cell Rep (2020) 32 pp. 108157-108157 [ DOI: doi:10.1016/j.celrep.2020.108157 Pubmed: 32937119 ]
- 50s ribosomal protein l4 (22 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l22 (12 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l21 (11 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l17 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l35 (7 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s12 (14 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s16 (9 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s17 (9 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s14 (7 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l20 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s6 (10 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s11 (11 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s9 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l23 (10 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s7 (16 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l5 (19 kDa, Protein from Bacillus subtilis (strain 168))
- Gtp pyrophosphokinase (39 kDa, Protein from Bacillus subtilis (strain 168))
- P-trna (28 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 16s rrna (496 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 5s ribosomal rna (36 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l29 (7 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s2 (25 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l6 (19 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l31 (7 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l28 (6 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l3 (22 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l27 (8 kDa, Protein from Bacillus subtilis (strain 168))
- 23s ribosomal rna (948 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l34 (5 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s13 (12 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s19 (8 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s10 (10 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l13 (16 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l33 1 (5 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s3 (23 kDa, Protein from Bacillus subtilis (strain 168))
- 30s ribosomal protein s18 (7 kDa, Protein from Bacillus subtilis (strain 168))
- A/r-trna (24 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l16 (15 kDa, Protein from Bacillus subtilis (strain 168))
- E-trna (24 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l24 (10 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l30 (6 kDa, Protein from Bacillus subtilis (strain 168))
- Bacillus subtilis subsp. subtilis str. 168 (Complex from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l2 (29 kDa, Protein from Bacillus subtilis (strain 168))
Cryo-EM reconstruction of the large subunit of the Mycobacterium smegmatis ribosome
Resolution: 3.18 Å
EM Method: Single-particle
Fitted PDBs: 5o60
Q-score: 0.487
Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N
Cell Rep (2017) 20 pp. 149-160 [ DOI: doi:10.1016/j.celrep.2017.06.029 Pubmed: 28683309 ]
- 50s ribosomal protein l6 (19 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l15 (15 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein bl37 (2 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l17 (21 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l4 (22 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l10 (18 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l19 (12 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l24 (11 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l2 (30 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l11 (15 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 23s rrna (1 MDa, RNA from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l28 (6 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l30 (7 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l22 (16 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l35 (7 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- Phenylalanine (165 Da, Ligand from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l29 (8 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l18 (13 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- Trna cca-end acetylated (phe) (894 Da, RNA from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l33 1 (6 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s large ribosomal subunit (Complex from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l14 (13 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l25 (22 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l27 (9 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l23 (11 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l3 (23 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l13 (16 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l36 (4 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l5 (21 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l34 (5 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l20 (14 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l9 (15 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l16 (15 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 5s rrna (38 kDa, RNA from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l21 (11 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l32 (6 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l31 (8 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
Cryo-EM reconstruction of the Mycobacterium smegmatis 70S ribosome
Hentschel J, Burnside C, Mignot I, Leibundgut M, Boehringer D, Ban N
Cell Rep (2017) 20 pp. 149-160 [ Pubmed: 28683309 DOI: doi:10.1016/j.celrep.2017.06.029 ]
- 30s ribosomal protein s20 (9 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s19 (10 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 5s rrna (38 kDa, RNA from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l27 (9 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 23s rrna (1 MDa, RNA from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein bl37 (2 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l35 (7 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s7 (17 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l23 (11 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s5 (21 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s6 (10 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l36 (4 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s15 (10 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l14 (13 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l13 (16 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l29 (8 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s3 (30 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s2 (30 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- Mrna fragment (1 kDa, RNA from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s17 (11 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l16 (15 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l11 (15 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- Lsu ribosomal protein l28p (6 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s14 type z (6 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l32 (6 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 16s rrna (495 kDa, RNA from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l15 (15 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- Phenylalanine (165 Da, Ligand from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l34 (5 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l17 (21 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s8 (14 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- Conserved domain protein (4 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- P/p-site phe-trna(phe) (24 kDa, RNA from Mycobacterium smegmatis str. MC2 155)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l24 (11 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l25 (22 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l20 (14 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l21 (11 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l33 1 (6 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l6 (19 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l18 (13 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l22 (16 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s4 (23 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l3 (23 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s11 (14 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s18 2 (9 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s10 (11 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l5 (21 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l2 (30 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l4 (22 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s12 (13 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s13 (14 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s9 (16 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l9 (15 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 30s ribosomal protein s16 (16 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 70s ribosome (Complex from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l30 (7 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l19 (12 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l10 (18 kDa, Protein from Mycobacterium smegmatis str. MC2 155)
- 50s ribosomal protein l31 (8 kDa, Protein from Mycobacterium smegmatis str. MC2 155)