Cutibacterium acnes 50S ribosomal subunit with Clindamycin bound
Lomakin IB, Devarkar SC, Grada A, Bunick CG
J Invest Dermatol. (2024) 144 pp. 2553-2561.e3 [ DOI: doi:10.1016/j.jid.2024.07.013 Pubmed: 39122144 ]
- 50s ribosomal protein l28 (6 kDa, Protein from Cutibacterium acnes)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Cutibacterium acnes)
- 23s rrna (1 MDa, RNA from Cutibacterium acnes)
- 50s ribosomal protein l2 (30 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l18 (13 kDa, Protein from Cutibacterium acnes)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l27 (9 kDa, Protein from Cutibacterium acnes)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l19 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l3 (23 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l32 (6 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l25 (22 kDa, Protein from Cutibacterium acnes)
- Clindamycin (424 Da, Ligand)
- 50s ribosomal protein l31 (7 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l36 (4 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l29 (8 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l16 (15 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l20 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l13 (16 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l34 (5 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l4 (32 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l14 (13 kDa, Protein from Cutibacterium acnes)
- 50s subunit with clindamycin bound (Complex from Cutibacterium acnes)
- 50s ribosomal protein l15 (15 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein bl37 (2 kDa, Protein from Cutibacterium acnes)
- Large ribosomal subunit protein ul24 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l6 (19 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l22 (16 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l23 (11 kDa, Protein from Cutibacterium acnes)
- Large ribosomal subunit protein bl17 (20 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l30 (6 kDa, Protein from Cutibacterium acnes)
- 5s rrna (38 kDa, RNA from Cutibacterium acnes)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l33 (6 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l5 (23 kDa, Protein from Cutibacterium acnes)
Cutibacterium acnes 70S ribosome with mRNA, P-site tRNA and Sarecycline bound
Resolution: 2.78 Å
EM Method: Single-particle
Fitted PDBs: 8crx
Q-score: 0.519
Lomakin IB, Devarkar SC, Patel S, Grada A, Bunick CG
Nucleic Acids Res (2023) 51 pp. 2915-2930 [ Pubmed: 36864821 DOI: doi:10.1093/nar/gkad103 ]
- 50s ribosomal protein l19 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l32 (6 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s11 (14 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s15 (10 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s13 (14 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s12 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l24 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l30 (6 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l31 (7 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l18 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l36 (4 kDa, Protein from Cutibacterium acnes)
- 5s ribosomal rna (38 kDa, RNA from Cutibacterium acnes)
- 30s ribosomal protein s16 (15 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s14 type z (6 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l25 (22 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l23 (11 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l33 (6 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l27 (9 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l5 (23 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s2 (31 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l20 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l2 (30 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l15 (15 kDa, Protein from Cutibacterium acnes)
- P-site initiator trna (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s6 (11 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s7 (17 kDa, Protein from Cutibacterium acnes)
- 32mf mrna (7 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s18 (8 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l17 (20 kDa, Protein from Cutibacterium acnes)
- Water (18 Da, Ligand)
- 30s ribosomal protein s17 (10 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l29 (8 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s8 (14 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein bl37 (2 kDa, Protein from Cutibacterium acnes)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s20 (9 kDa, Protein from Cutibacterium acnes)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s4 (23 kDa, Protein from Cutibacterium acnes)
- 70s ribosome with mrna, p-site trna and sarecycline bound (Complex from Cutibacterium acnes)
- 30s ribosomal protein s9 (18 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l4 (32 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l35 (7 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l34 (5 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l13 (16 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l14 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l28 (6 kDa, Protein from Cutibacterium acnes)
- 23s ribosomal rna (1 MDa, RNA from Cutibacterium acnes)
- Rna (1537-mer) (498 kDa, RNA from Cutibacterium acnes)
- 30s ribosomal protein s5 (22 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s19 (10 kDa, Protein from Cutibacterium acnes)
- Sarecycline (487 Da, Ligand)
- 50s ribosomal protein l21 (11 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l3 (23 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s3 (29 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l16 (15 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l6 (19 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l22 (16 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein bs22 (4 kDa, Protein from Cutibacterium acnes)
- 30s ribosomal protein s10 (11 kDa, Protein from Cutibacterium acnes)
Resolution: 2.66 Å
EM Method: Single-particle
Fitted PDBs: 8cvm
Q-score: 0.565
Lomakin IB, Devarkar SC, Patel S, Grada A, Bunick CG
Nucleic Acids Res (2023) 51 pp. 2915-2930 [ DOI: doi:10.1093/nar/gkad103 Pubmed: 36864821 ]
- 23s ribosomal rna (1 MDa, RNA from Cutibacterium acnes)
- 50s ribosomal protein l28 (6 kDa, Protein from Cutibacterium acnes)
- Initiator fmet rna (24 kDa, RNA from Escherichia coli)
- 50s subunit from 70s ribosome with mrna, p-site trna and sarecycline bound (Complex from Cutibacterium acnes)
- 50s ribosomal protein l2 (30 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l18 (13 kDa, Protein from Cutibacterium acnes)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l27 (9 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l19 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l3 (23 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l32 (6 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l25 (22 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l31 (7 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l36 (4 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l29 (8 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l16 (15 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l35 (7 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l20 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l13 (16 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l34 (5 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l21 (11 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l24 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l4 (32 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l14 (13 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l17 (20 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l15 (15 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein bl37 (2 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l6 (19 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l22 (16 kDa, Protein from Cutibacterium acnes)
- Sarecycline (487 Da, Ligand)
- 5s ribosomal rna (38 kDa, RNA from Cutibacterium acnes)
- 50s ribosomal protein l23 (11 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l30 (6 kDa, Protein from Cutibacterium acnes)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l33 (6 kDa, Protein from Cutibacterium acnes)
- 50s ribosomal protein l5 (23 kDa, Protein from Cutibacterium acnes)