Cryo-EM structure of the Acinetobacter baumannii Ribosome: 70S with P-site occupied
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 6v39
Q-score: 0.484
Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW
Mbio (2020) 11 [ DOI: doi:10.1128/mBio.03117-19 Pubmed: 31964740 ]
- 50s ribosomal protein l28 (9 kDa, Protein from Acinetobacter rudis CIP 110305)
- Mrna 5'-aug-3' (935 Da, RNA from Acinetobacter baumannii AB0057)
- 30s ribosomal protein s9 (14 kDa, Protein from Acinetobacter baumannii)
- 30s ribosomal protein s2 (27 kDa, Protein from Acinetobacter baumannii)
- Sodium ion (22 Da, Ligand)
- 50s ribosomal protein l27 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s6 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 23s ribosomal rna (945 kDa, RNA from Acinetobacter baumannii AB0057)
- 50s ribosomal protein l33 (6 kDa, Protein from Acinetobacter sp. CIP 51.11)
- 50s ribosomal protein l22 (11 kDa, Protein from Acinetobacter sp. 809848)
- 50s ribosomal protein l25 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s10 (11 kDa, Protein from Acinetobacter sp. ANC 5600)
- 50s ribosomal protein l14 (13 kDa, Protein from Acinetobacter sp. NIPH 899)
- 50s ribosomal protein l29 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s13 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s11 (13 kDa, Protein from Acinetobacter sp. 263903-1)
- 30s ribosomal protein s5 (17 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 16s ribosomal rna (500 kDa, RNA from Acinetobacter baumannii AB0057)
- 30s ribosomal protein s14 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l5 (20 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s16 (11 kDa, Protein from Acinetobacter baumannii)
- 50s ribosomal protein l30 (6 kDa, Protein from Acinetobacter sp. CIP 102082)
- 50s ribosomal protein l32 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l16 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s19 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Water (18 Da, Ligand)
- 50s ribosomal protein l3 (22 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 5s ribosomal rna (36 kDa, RNA from Acinetobacter baumannii)
- 50s ribosomal protein l20 (13 kDa, Protein from Acinetobacter baumannii)
- 50s ribosomal protein l21 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l13 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s12 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s18 (9 kDa, Protein from Acinetobacter venetianus)
- 30s ribosomal protein s17 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l4 (21 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Acinetobacter baumannii 70s ribosome with p-site trna (2 MDa, Complex from Acinetobacter baumannii AB0057)
- 30s ribosomal protein s4 (23 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s15 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Trna-met (24 kDa, RNA from Acinetobacter baumannii AB0057)
- N-formylmethionine (177 Da, Ligand)
- 50s ribosomal protein l35 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l18 (12 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s8 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l2 (30 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l24 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s7 (17 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s20 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l15 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l34 (5 kDa, Protein from Acinetobacter beijerinckii ANC 3835)
- 50s ribosomal protein l9 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l36 (4 kDa, Protein from Acinetobacter sp. ANC 4470)
- 50s ribosomal protein l19 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l23 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s3 (27 kDa, Protein from Acinetobacter baumannii)
- 50s ribosomal protein l17 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s21 (8 kDa, Protein from Acinetobacter sp. 263903-1)
- 50s ribosomal protein l6 (19 kDa, Protein from Acinetobacter baumannii (strain AB0057))
Cryo-EM structure of the Acinetobacter baumannii Ribosome: 70S with E-site tRNA
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 6v3a
Q-score: 0.531
Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW
Mbio (2020) 11 [ Pubmed: 31964740 DOI: doi:10.1128/mBio.03117-19 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Acinetobacter sp. ANC 5600)
- 30s ribosomal protein s15 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Trna-met (24 kDa, RNA from Acinetobacter baumannii AB0057)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l30 (6 kDa, Protein from Acinetobacter sp. CIP 102082)
- 50s ribosomal protein l32 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l17 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l24 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 23s ribosomal rna (945 kDa, RNA from Acinetobacter baumannii AB0057)
- 30s ribosomal protein s9 (14 kDa, Protein from Acinetobacter baumannii)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l6 (19 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l22 (11 kDa, Protein from Acinetobacter sp. 809848)
- 30s ribosomal protein s19 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s17 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s18 (9 kDa, Protein from Acinetobacter venetianus)
- 50s ribosomal protein l9 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s13 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Mrna (935 Da, RNA from Acinetobacter baumannii AB0057)
- 50s ribosomal protein l33 (6 kDa, Protein from Acinetobacter sp. CIP 51.11)
- 30s ribosomal protein s11 (13 kDa, Protein from Acinetobacter sp. 263903-1)
- 50s ribosomal protein l21 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l34 (5 kDa, Protein from Acinetobacter beijerinckii ANC 3835)
- 50s ribosomal protein l18 (12 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l4 (21 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Sodium ion (22 Da, Ligand)
- 30s ribosomal protein s7 (17 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l19 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l5 (20 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l29 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s2 (27 kDa, Protein from Acinetobacter baumannii)
- 30s ribosomal protein s5 (17 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l20 (13 kDa, Protein from Acinetobacter baumannii)
- 50s ribosomal protein l3 (22 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s16 (11 kDa, Protein from Acinetobacter baumannii)
- 50s ribosomal protein l35 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l2 (30 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 16s ribosomal rna (500 kDa, RNA from Acinetobacter baumannii)
- 50s ribosomal protein l25 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l27 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s20 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l23 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l28 (9 kDa, Protein from Acinetobacter rudis CIP 110305)
- 30s ribosomal protein s4 (23 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s12 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 5s ribosomal rna (36 kDa, RNA from Acinetobacter baumannii)
- 50s ribosomal protein l13 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l15 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l14 (13 kDa, Protein from Acinetobacter sp. NIPH 899)
- 30s ribosomal protein s3 (27 kDa, Protein from Acinetobacter baumannii)
- 30s ribosomal protein s8 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s14 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s21 (8 kDa, Protein from Acinetobacter sp. 263903-1)
- 50s ribosomal protein l36 (4 kDa, Protein from Acinetobacter sp. ANC 4470)
- 50s ribosomal protein l16 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Acinetobacter baumannii 70s ribosome with e-site trna bound (2 MDa, Complex from Acinetobacter baumannii AB0057)
- 30s ribosomal protein s6 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
Cryo-EM structure of the Acinetobacter baumannii Ribosome: 70S in Empty state
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 6v3b
Q-score: 0.515
Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW
Mbio (2020) 11 [ DOI: doi:10.1128/mBio.03117-19 Pubmed: 31964740 ]
- 50s ribosomal protein l28 (9 kDa, Protein from Acinetobacter rudis CIP 110305)
- 30s ribosomal protein s9 (14 kDa, Protein from Acinetobacter baumannii)
- 30s ribosomal protein s2 (27 kDa, Protein from Acinetobacter baumannii)
- Sodium ion (22 Da, Ligand)
- 50s ribosomal protein l27 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s6 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 23s ribosomal rna (945 kDa, RNA from Acinetobacter baumannii AB0057)
- 50s ribosomal protein l33 (6 kDa, Protein from Acinetobacter sp. CIP 51.11)
- 50s ribosomal protein l22 (11 kDa, Protein from Acinetobacter sp. 809848)
- 50s ribosomal protein l25 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s10 (11 kDa, Protein from Acinetobacter sp. ANC 5600)
- 50s ribosomal protein l14 (13 kDa, Protein from Acinetobacter sp. NIPH 899)
- 50s ribosomal protein l29 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s13 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s11 (13 kDa, Protein from Acinetobacter sp. 263903-1)
- 30s ribosomal protein s5 (17 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 16s ribosomal rna (500 kDa, RNA from Acinetobacter baumannii AB0057)
- 30s ribosomal protein s14 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l5 (20 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s16 (11 kDa, Protein from Acinetobacter baumannii)
- 50s ribosomal protein l30 (6 kDa, Protein from Acinetobacter sp. CIP 102082)
- 50s ribosomal protein l32 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l16 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s19 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Water (18 Da, Ligand)
- 50s ribosomal protein l3 (22 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 5s ribosomal rna (36 kDa, RNA from Acinetobacter baumannii)
- 50s ribosomal protein l20 (13 kDa, Protein from Acinetobacter baumannii)
- 50s ribosomal protein l21 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l13 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s12 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s18 (9 kDa, Protein from Acinetobacter venetianus)
- 30s ribosomal protein s17 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l4 (21 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s4 (23 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s15 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l35 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l18 (12 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s8 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l2 (30 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l24 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s7 (17 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s20 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Acinetobacter baumannii 70s ribosome with no trna bound (2 MDa, Complex from Acinetobacter baumannii AB0057)
- 50s ribosomal protein l34 (5 kDa, Protein from Acinetobacter beijerinckii ANC 3835)
- 50s ribosomal protein l15 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l9 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l36 (4 kDa, Protein from Acinetobacter sp. ANC 4470)
- 50s ribosomal protein l19 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l23 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s3 (27 kDa, Protein from Acinetobacter baumannii)
- 50s ribosomal protein l17 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 30s ribosomal protein s21 (8 kDa, Protein from Acinetobacter sp. 263903-1)
- 50s ribosomal protein l6 (19 kDa, Protein from Acinetobacter baumannii (strain AB0057))
Cryo-EM structure of the Acinetobacter baumannii Ribosome: 50S subunit
Resolution: 2.95 Å
EM Method: Single-particle
Fitted PDBs: 6v3d
Q-score: 0.493
Morgan CE, Huang W, Rudin SD, Taylor DJ, Kirby JE, Bonomo RA, Yu EW
Mbio (2020) 11 [ DOI: doi:10.1128/mBio.03117-19 Pubmed: 31964740 ]
- 50s ribosomal protein l28 (9 kDa, Protein from Acinetobacter rudis CIP 110305)
- Sodium ion (22 Da, Ligand)
- 50s ribosomal protein l27 (9 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 23s ribosomal rna (945 kDa, RNA from Acinetobacter baumannii AB0057)
- 50s ribosomal protein l33 (6 kDa, Protein from Acinetobacter sp. CIP 51.11)
- 50s ribosomal protein l22 (11 kDa, Protein from Acinetobacter sp. 809848)
- 50s ribosomal protein l25 (10 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l14 (13 kDa, Protein from Acinetobacter sp. NIPH 899)
- 50s ribosomal protein l29 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Acinetobacter baumannii large ribosomal subunit (2 MDa, Complex from Acinetobacter baumannii AB0057)
- 50s ribosomal protein l5 (20 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l30 (6 kDa, Protein from Acinetobacter sp. CIP 102082)
- 50s ribosomal protein l32 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l16 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Water (18 Da, Ligand)
- 50s ribosomal protein l3 (22 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 5s ribosomal rna (36 kDa, RNA from Acinetobacter baumannii)
- 50s ribosomal protein l20 (13 kDa, Protein from Acinetobacter baumannii)
- 50s ribosomal protein l21 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l13 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- Zinc ion (65 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l4 (21 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l35 (7 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l18 (12 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l2 (30 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l24 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l15 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l34 (5 kDa, Protein from Acinetobacter beijerinckii ANC 3835)
- 50s ribosomal protein l9 (15 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l36 (4 kDa, Protein from Acinetobacter sp. ANC 4470)
- 50s ribosomal protein l19 (13 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l23 (11 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l17 (14 kDa, Protein from Acinetobacter baumannii (strain AB0057))
- 50s ribosomal protein l6 (19 kDa, Protein from Acinetobacter baumannii (strain AB0057))