Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7otc
Q-score: 0.504
Wieland M, Holm M, Rundlet EJ, Morici M, Koller TO, Maviza TP, Pogorevc D, Osterman IA, Muller R, Blanchard SC, Wilson DN
PNAS (2022) 119 pp. e2114214119-e2114214119 [ Pubmed: 35500116 DOI: doi:10.1073/pnas.2114214119 ]
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli BL21(DE3))
- Zinc ion (65 Da, Ligand)
- Elongation factor g (77 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s6 (12 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 1,4-diaminobutane (88 Da, Ligand)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli BL21(DE3))
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli BL21(DE3))
- Cryo-em structure of an escherichia coli 70s ribosome in complex with elongation factor g and the antibiotic argyrinb (Complex from Escherichia coli BL21(DE3))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli BL21(DE3))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli BL21(DE3))
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 16s ribosomal rna (497 kDa, RNA from Escherichia coli BL21(DE3))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli BL21(DE3))
- Spermidine (145 Da, Ligand)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli BL21(DE3))
- Argyrin b (838 Da, Ligand)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli BL21(DE3))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli BL21(DE3))
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli BL21(DE3))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli BL21(DE3))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli BL21(DE3))
Structure of translocated trans-translation complex on E. coli stalled ribosome.
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7acj
Q-score: 0.422
Guyomar C, D'Urso G, Chat S, Giudice E, Gillet R
Nat Commun (2021) 12 pp. 4909-4909 [ Pubmed: 34389707 DOI: doi:10.1038/s41467-021-24881-4 ]
- 30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
- Nascent peptide (236 Da, Protein from Escherichia coli K-12)
- Structure of translocated trans-translation complex on e. coli stalled ribosome. (Complex)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli K-12)
- Ssra-binding protein (17 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli K-12)
- Transfer-messanger rna (tmrna) (117 kDa, RNA from Escherichia coli BL21(DE3))
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 16s ribosomal rna (497 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- Ribosome (Complex from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s2 (25 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli K-12)
- Smpb (Complex from Escherichia coli K-12)
- Ribosomal rna and nascent peptide (Complex from Escherichia coli K-12)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s6 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- Rna (Complex from Escherichia coli BL21(DE3))
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli K-12)