Cryo-EM structure of a native ribosome-Ski2-Ski3-Ski8 complex from S. cerevisiae
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5mc6
Q-score: 0.362
Schmidt C, Kowalinski E, Shanmuganathan V, Defenouillere Q, Braunger K, Heuer A, Pech M, Namane A, Berninghausen O, Fromont-Racine M, Jacquier A, Conti E, Becker T, Beckmann R
Science (2016) 354 pp. 1431-1433 [ DOI: doi:10.1126/science.aaf7520 Pubmed: 27980209 ]
- 60s ribosomal protein l31-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s3 (26 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l25 (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l13-a (22 kDa, Protein from Baker's yeast)
- 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l35-a (13 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l11-a (19 kDa, Protein from Baker's yeast)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Baker's yeast)
- Eukaryotic translation initiation factor 5a-1 (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s13 (17 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s24-a (15 kDa, Protein from Baker's yeast)
- Ul1 (17 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l10 (25 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s1-a (28 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l43-a (10 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l16-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s10-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s30-a (7 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l7-a (27 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l42-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s20 (13 kDa, Protein from Baker's yeast)
- Native ribosome-ski2-ski3-ski8 complex from s. cerevisiae (3 MDa, Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l15-a (24 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s2 (27 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s7-a (21 kDa, Protein from Baker's yeast)
- Antiviral helicase ski2 (146 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s4-a (29 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l4-a (39 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l28 (16 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l36-a (11 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l39 (6 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l23-a (14 kDa, Protein from Baker's yeast)
- 18s ribosomal rna (579 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l41-a (3 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l38 (8 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s8-a (22 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s26-a (13 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s11-a (17 kDa, Protein from Baker's yeast)
- 5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l32 (14 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l26-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s12 (15 kDa, Protein from Baker's yeast)
- Superkiller protein 3 (163 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l17-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l5 (33 kDa, Protein from Baker's yeast)
- A-site trna (24 kDa, RNA from Baker's yeast)
- P-site trna (24 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s15 (16 kDa, Protein from Baker's yeast)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l18-a (20 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s17-b (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l6-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l3 (43 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l19-a (21 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l34-a (13 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s28-b (7 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s27-a (8 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s29-a (6 kDa, Protein from Baker's yeast)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s23-a (16 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l20-a (20 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l30 (11 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s5 (25 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s6-a (27 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s22-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s19-a (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l27-a (15 kDa, Protein from Baker's yeast)
- Mrna (10 kDa, RNA from Baker's yeast)
- 40s ribosomal protein s25-a (12 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s16-a (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l2-a (27 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l14-a (15 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l37-a (9 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l22-a (13 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l24-a (17 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l29 (6 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s14-a (14 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s0-a (28 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l8-a (28 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l33-a (12 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l9-a (21 kDa, Protein from Baker's yeast)
- 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s18-a (17 kDa, Protein from Baker's yeast)
- Antiviral protein ski8 (44 kDa, Protein from Baker's yeast)
- 40s ribosomal protein s9-a (22 kDa, Protein from Baker's yeast)
- 60s ribosomal protein l21-a (18 kDa, Protein from Baker's yeast)
Nonstop ribosomal complex bound with Dom34 and Hbs1
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 5m1j
Q-score: 0.45
Hilal T, Yamamoto H, Loerke J, Burger J, Mielke T, Spahn CM
Nat Commun (2016) 7 pp. 13521-13521 [ Pubmed: 27995908 DOI: doi:10.1038/ncomms13521 ]
- 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s15 (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l42-b (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l6-a (19 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l8-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Saccharomyces cerevisiae)
- Nonstop mrna (6 kDa, RNA from synthetic construct)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-40s ribosomal protein s31 (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s3 (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s20 (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s0-a (22 kDa, Protein from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l34-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l24-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae)
- 5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l41-b (3 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (23 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l7-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s17-b (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- 40s ribosomal protein s28-b (7 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Water (18 Da, Ligand)
- 40s ribosomal protein s13 (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s10-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s5 (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l13-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s12 (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Protein dom34 (43 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s26-b (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l43-b (9 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- N-acetyl-l-phenylalanine (207 Da, Ligand)
- 60s ribosomal protein l30 (10 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Nonstop ribosomal complex bound with dom34-hbs1-gmppnp (3 MDa, Complex)
- 60s ribosomal protein l22-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 18s ribosomal rna (579 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s25-a (8 kDa, Protein from Saccharomyces cerevisiae)
- Yeast phe-trna-phe (24 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s18-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l25 (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Protein hbs1 (68 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae)