Cryo-EM structure of a nucleolar pre-60S ribosome (Rpf1-TAP)
Resolution: 3.65 Å
EM Method: Single-particle
Fitted PDBs: 5z3g
Q-score: 0.422
Zhou D, Zhu X, Zheng S, Tan D, Dong MQ, Ye K
Protein Cell (2019) 10 pp. 120-130 [ Pubmed: 29557065 DOI: doi:10.1007/s13238-018-0526-7 ]
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae S288c)
- Protein mak16 (35 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae S288c)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae S288c)
- Rpf1-tap pre-60s (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein nsa1 (51 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
- Nucleolar protein 7 (69 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosomal rna-processing protein 1 (33 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae S288c)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae S288c)
- Ribosome production factor 1 (35 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae S288c)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae S288c)
- Unassigned (13 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae S288c)
- Its2 rna (74 kDa, RNA from Saccharomyces cerevisiae)
- Rrna-processing protein ebp2 (49 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae S288c)
Yeast nucleolar pre-60S ribosomal subunit (state 2)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6c0f
Q-score: 0.339
Sanghai ZA, Miller L, Molloy KR, Barandun J, Hunziker M, Chaker-Margot M, Wang J, Chait BT, Klinge S
Nature (2018) 556 pp. 126-129 [ Pubmed: 29512650 DOI: doi:10.1038/nature26156 ]
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal rna-processing protein 14 (50 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Protein mak16 (35 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein ssf1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal rna-processing protein 15 (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Its2 (74 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal rna-processing protein 1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Yeast large subunit processome (Complex from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein ebp2 (6 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Ribosome production factor 1 (35 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Brx1-associated peptide (2 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Protein mak11 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar protein 16 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Saccharomyces cerevisiae s288c 35s pre-ribosomal rna miscrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (89 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)