Cryo-EM structure of the mRNA translating and degrading yeast 80S ribosome-Xrn1 nuclease complex
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6q8y
Q-score: 0.484
Tesina P, Heckel E, Cheng J, Fromont-Racine M, Buschauer R, Kater L, Beatrix B, Berninghausen O, Jacquier A, Becker T, Beckmann R
Nat Struct Mol Biol (2019) 26 pp. 275-280 [ Pubmed: 30911188 DOI: doi:10.1038/s41594-019-0202-5 ]
- 40s ribosomal protein s12 (13 kDa, Protein from Saccharomyces cerevisiae)
- 25s ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 5'-3' exoribonuclease xrn1 (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l6-a (19 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l8-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s26-b (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l24-a (7 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l43-a (9 kDa, Protein from Saccharomyces cerevisiae)
- P-site trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s0-a (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae)
- Mrna (5 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s3 (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s20 (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s ribosomal rna (50 kDa, RNA from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l34-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae)
- E-site trna (Complex from Saccharomyces cerevisiae)
- 5s ribosomal rna (38 kDa, RNA from Saccharomyces cerevisiae)
- Ubiquitin-40s ribosomal protein s31 (5 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-b (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s15 (13 kDa, Protein from Saccharomyces cerevisiae)
- Rps5p (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (24 kDa, Protein from Saccharomyces cerevisiae)
- Mrna (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l11-b (19 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l41-b (3 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l7-a (25 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s17-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s18-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae)
- E-site trna (24 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (16 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s10-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l13-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae)
- Ribosome (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l30 (10 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l22-a (11 kDa, Protein from Saccharomyces cerevisiae)
- P-site trna (Complex from Saccharomyces cerevisiae)
- 40s ribosomal protein s25-a (8 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 5'-3' exoribonuclease 1 (107 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l25 (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (23 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 18s ribosomal rna (578 kDa, RNA from Saccharomyces cerevisiae)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae)
- Yeast 80s ribosome in complex with the xrn11 exoribonuclease obtained from natural source. (Complex)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae)