Cryo-EM structure of yeast Lso2 bound to 80S ribosomes under native condition
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6z6j
Q-score: 0.482
Wells JN, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, Becker T, Gilbert W, Cheng J, Beckmann R
PLoS Biol (2020) 18 pp. e3000780-e3000780 [ DOI: doi:10.1371/journal.pbio.3000780 Pubmed: 32687489 ]
- 40s ribosomal protein s3 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s25-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein lso2 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s26-b (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s10-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s15 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Lso2-80s ribosome (Complex from Saccharomyces cerevisiae ATCC 204508 / S288c)
- 40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l41-b (3 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l24-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 18s rrna (579 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Cryo-EM structure of yeast reconstituted Lso2 bound to 80S ribosomes
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 6z6k
Q-score: 0.494
Wells JN, Buschauer R, Mackens-Kiani T, Best K, Kratzat H, Berninghausen O, Becker T, Gilbert W, Cheng J, Beckmann R
PLoS Biol (2020) 18 pp. e3000780-e3000780 [ Pubmed: 32687489 DOI: doi:10.1371/journal.pbio.3000780 ]
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Protein lso2 (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s26-b (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l24-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s25-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s10-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s15 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l41-a (3 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Lso2 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 80s ribosome (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s28-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Lso2-80s ribosome (Complex)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Rps5p (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s17-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 18s rrna (579 kDa, RNA from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s3 (26 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))