Cryo-EM structure of stalled rabbit 80S ribosomes in complex with human CCR4-NOT and CNOT4
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8bhf
Q-score: 0.492
Gangwar SP, Yen LY, Yelshanskaya MV, Korman A, Jones DR, Sobolevsky AI
Nat Struct Mol Biol (2023) 30 pp. 1481-1494 [ Pubmed: 37653241 DOI: doi:10.1038/s41594-023-01080-x ]
- Ribosomal protein l19 (21 kDa, Protein from Oryctolagus cuniculus)
- P-site trna (24 kDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l23 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s8 (24 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s3 (25 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27a (16 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s7 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l18a (20 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l38 (8 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l5 (34 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s6 (27 kDa, Protein from Oryctolagus cuniculus)
- Plectin/s10 n-terminal domain-containing protein (11 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein us15 (17 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s18 (16 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l31 (12 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s27 (9 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s21 (9 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l35 (14 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s16 (16 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s29 (6 kDa, Protein from Oryctolagus cuniculus)
- Ccr4-not transcription complex subunit 3 (28 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (41 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l41 (3 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s4 (29 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l36 (11 kDa, Protein from Oryctolagus cuniculus)
- Ubiquitin-like domain-containing protein (6 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s17 (15 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l11 (19 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l22 (11 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l12 (16 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l39-like (6 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s9 (predicted) (21 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s11 (17 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27 (15 kDa, Protein from Oryctolagus cuniculus)
- 60s acidic ribosomal protein p0 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l6 (28 kDa, Protein from Oryctolagus cuniculus)
- Trash domain-containing protein (12 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s28 (7 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l34 (12 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s25 (8 kDa, Protein from Oryctolagus cuniculus)
- Ribosome complex (Complex from Oryctolagus cuniculus)
- 40s ribosomal protein s2 (24 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l9 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l28 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s3a (24 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s15a (14 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l10 (24 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l26 (15 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s5 (21 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l15 (24 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein rack1 (34 kDa, Protein from Oryctolagus cuniculus)
- Mrna (896 Da, RNA from Oryctolagus cuniculus)
- Ribosomal protein l37 (10 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s15 (15 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l8 (26 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l35a (12 kDa, Protein from Oryctolagus cuniculus)
- Nascent chain (501 Da, Protein from Oryctolagus cuniculus)
- 40s_sa_c domain-containing protein (24 kDa, Protein from Oryctolagus cuniculus)
- Ubiquitin-40s ribosomal protein s27a (7 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s19 (15 kDa, Protein from Oryctolagus cuniculus)
- 18s ribosomal rna (547 kDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l17 (17 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l23/l25 n-terminal domain-containing protein (13 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s12 (13 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l10a (23 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l18 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l29 (13 kDa, Protein from Oryctolagus cuniculus)
- 5s ribosomal rna (38 kDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l13a (23 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l7 (26 kDa, Protein from Oryctolagus cuniculus)
- 5.8s ribosomal rna (50 kDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l14 (16 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s23 (15 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l13 (24 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l7a (27 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l37a (10 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l3 (45 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l32 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s26 (11 kDa, Protein from Oryctolagus cuniculus)
- 28s ribosomal rna (1 MDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l30 (10 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l21 (18 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l36a like (12 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s14 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s24 (14 kDa, Protein from Oryctolagus cuniculus)
- Ubiquitin-60s ribosomal protein l40 (6 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s20 (11 kDa, Protein from Oryctolagus cuniculus)
Structure of the TRAP complex with the Sec translocon and a translating ribosome
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8btk
Q-score: 0.362
Jaskolowski M, Jomaa A, Gamerdinger M, Shrestha S, Leibundgut M, Deuerling E, Ban N
Nat Struct Mol Biol (2023) 30 pp. 770-777 [ Pubmed: 37170030 DOI: doi:10.1038/s41594-023-00990-0 ]
- 60s ribosomal protein l9 (21 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s9 (predicted) (22 kDa, Protein from Oryctolagus cuniculus)
- El38 (8 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l6 (33 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l22 (14 kDa, Protein from Oryctolagus cuniculus)
- 60s acidic ribosomal protein p0 (34 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein ul1 (24 kDa, Protein from Oryctolagus cuniculus)
- El30 (12 kDa, Protein from Oryctolagus cuniculus)
- Ubiquitin (14 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l26 (17 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l7a (30 kDa, Protein from Oryctolagus cuniculus)
- Us9 (19 kDa, Protein from Oryctolagus cuniculus)
- Acetylamino-acetic acid (117 Da, Ligand)
- Ribosomal protein l15 (24 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s3a (30 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s24 (15 kDa, Protein from Oryctolagus cuniculus)
- Spermidine (145 Da, Ligand)
- 40s ribosomal protein s25 (13 kDa, Protein from Oryctolagus cuniculus)
- Us14 (6 kDa, Protein from Oryctolagus cuniculus)
- Us15 (17 kDa, Protein from Oryctolagus cuniculus)
- Nascent chain (2 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6 (28 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal large subunit (60s) (Complex from Oryctolagus cuniculus)
- El39 (6 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s21 (9 kDa, Protein from Oryctolagus cuniculus)
- L13 (24 kDa, Protein from Oryctolagus cuniculus)
- Translocon-associated protein subunit delta (18 kDa, Protein from Canis lupus familiaris)
- 60s ribosomal protein l35a (12 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s5 (22 kDa, Protein from Oryctolagus cuniculus)
- 5s rrna (38 kDa, RNA from Oryctolagus cuniculus)
- 40s ribosomal protein s27 (9 kDa, Protein from Oryctolagus cuniculus)
- Signal sequence receptor subunit 3 (21 kDa, Protein from Canis lupus familiaris)
- 60s ribosomal protein l29 (26 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s11 (18 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal_s5_c domain-containing protein,40s ribosomal protein s2 (31 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l3 (46 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l11 (20 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l23 (14 kDa, Protein from Oryctolagus cuniculus)
- Mrna (1 kDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l27a (16 kDa, Protein from Oryctolagus cuniculus)
- El42 (12 kDa, Protein from Oryctolagus cuniculus)
- Ul30,60s ribosomal protein l7 (29 kDa, Protein from Oryctolagus cuniculus)
- Spermine (202 Da, Ligand)
- 40s ribosomal protein sa (32 kDa, Protein from Oryctolagus cuniculus)
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Canis lupus familiaris)
- El32 (15 kDa, Protein from Oryctolagus cuniculus)
- Structure of the trap complex with the sec translocon and a translating ribosome (Complex from Oryctolagus cuniculus)
- 40s ribosomal protein s3 (31 kDa, Protein from Oryctolagus cuniculus)
- P-site trna (24 kDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l34 (13 kDa, Protein from Oryctolagus cuniculus)
- Translocon-associated protein subunit beta (20 kDa, Protein from Canis lupus familiaris)
- 60s ribosomal protein l18a (20 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l24 (17 kDa, Protein from Oryctolagus cuniculus)
- P-site trna (Complex from Oryctolagus cuniculus)
- Sec61 translocon (Complex from canis lupus familiaris)
- Es26 (13 kDa, Protein from Oryctolagus cuniculus)
- 5.8s rrna (50 kDa, RNA from Oryctolagus cuniculus)
- 40s ribosomal protein s7 (47 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s14 (16 kDa, Protein from Oryctolagus cuniculus)
- Ul22 (21 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l36 (12 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l14 (23 kDa, Protein from Oryctolagus cuniculus)
- Zinc ion (65 Da, Ligand)
- El43 (10 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal small subunit (ssu) (Complex from Oryctolagus cuniculus)
- 60s ribosomal protein l19 (23 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l27 (15 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein es10 (18 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l8 (28 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l12 (17 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s4 (29 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s23 (15 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s27a (18 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s28 (7 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein s15a (14 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l37 (11 kDa, Protein from Oryctolagus cuniculus)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Canis lupus familiaris)
- 40s ribosomal protein s12 (14 kDa, Protein from Oryctolagus cuniculus)
- Trap complex (Complex from canis lupus familiaris)
- 60s ribosomal protein l35 (14 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s8 (24 kDa, Protein from Oryctolagus cuniculus)
- 18s rrna (603 kDa, RNA from Oryctolagus cuniculus)
- 40s ribosomal protein es17 (15 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s30 (14 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal_l18_c domain-containing protein (34 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal_l23en domain-containing protein (17 kDa, Protein from Oryctolagus cuniculus)
- 28s rrna (1 MDa, RNA from Oryctolagus cuniculus)
- 60s ribosomal protein l13a (23 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l28 (15 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l4 (46 kDa, Protein from Oryctolagus cuniculus)
- Rack1 (35 kDa, Protein from Oryctolagus cuniculus)
- El18 (21 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us10 (13 kDa, Protein from Oryctolagus cuniculus)
- 60s ribosomal protein l41 (3 kDa, Protein from Oryctolagus cuniculus)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Canis lupus familiaris)
- 40s ribosomal protein s19 (16 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein us19 (17 kDa, Protein from Oryctolagus cuniculus)
- 40s ribosomal protein s18 (17 kDa, Protein from Oryctolagus cuniculus)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l21 (18 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l31 (14 kDa, Protein from Oryctolagus cuniculus)
- Ribosomal protein l10 (24 kDa, Protein from Oryctolagus cuniculus)
- Translocon-associated protein subunit alpha (32 kDa, Protein from Canis lupus familiaris)
Cryo-EM structure of pre-60S ribosomal subunit, unmethylated G2922
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7ug6
Q-score: 0.533
Yelland JN, Bravo JPK, Black JJ, Taylor DW, Johnson AW
Nat Struct Mol Biol (2023) 30 pp. 91-98 [ DOI: doi:10.1038/s41594-022-00891-8 Pubmed: 36536102 ]
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein rpf2 (39 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nucleolar gtp-binding protein 2 (59 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Regulator of ribosome biosynthesis (23 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein alb1 (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l12-a (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome assembly protein 4 (56 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Nucle(ol)ar intermediate of large ribosomal subunit biogenesis, immunopurified by nog2-3xflag (Complex from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Bud site selection protein 20 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
State NE1 nucleolar 60S ribosome biogenesis intermediate - Overall map
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7u0h
Q-score: 0.592
Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP
Nat Struct Mol Biol (2022) 29 pp. 1228-1238 [ Pubmed: 36482249 DOI: doi:10.1038/s41594-022-00874-9 ]
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 2 (1 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Its2 rrna (27 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 27s pre-rrna (guanosine(2922)-2'-o)-methyltransferase (96 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nop53 (52 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Early nucleoplasmic 60s intermediate purified with tags on nop53 and spb1. (2 MDa, Complex from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
State E2 nucleolar 60S ribosomal biogenesis intermediate - Overall map
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7nac
Q-score: 0.549
Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP
Nat Struct Mol Biol (2022) 29 pp. 1228-1238 [ DOI: doi:10.1038/s41594-022-00874-9 Pubmed: 36482249 ]
- 60s ribosomal subunit assembly/export protein loc1 (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar protein 16 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein ebp2 (49 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Noc2 isoform 1 (81 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ytm1 isoform 1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on ytm1 and spb4. (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
- 5.8s ribosomal rna gene and internal transcribed spacer 2 (27 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal protein (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 27s pre-rrna (guanosine(2922)-2'-o)-methyltransferase (96 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex-associated protein 3 (75 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rrna helicase spb4 (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (91 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Rrp17 isoform 1 (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosome subunit biogenesis protein nip7 (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (cytosine(2870)-c(5))-methyltransferase (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
State E2 nucleolar 60S ribosomal biogenesis intermediate - Spb4 local refinement model
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7nad
Q-score: 0.547
Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP
Nat Struct Mol Biol (2022) 29 pp. 1228-1238 [ DOI: doi:10.1038/s41594-022-00874-9 Pubmed: 36482249 ]
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Noc3 isoform 1 (75 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Noc2 isoform 1 (81 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rpl8a isoform 1 (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rpl38 isoform 1 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ytm1 isoform 1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l23-a (4 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Spb4 isoform 1 (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (225 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Spb1 isoform 1 (96 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rlp7 isoform 1 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (48 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrp17 isoform 1 (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (23 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on ytm1 and spb4. (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
State E1 nucleolar 60S ribosome biogenesis intermediate - Spb4 locally refined map
Resolution: 3.07 Å
EM Method: Single-particle
Fitted PDBs: 7r72
Q-score: 0.519
Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP
Nat Struct Mol Biol (2022) 29 pp. 1228-1238 [ DOI: doi:10.1038/s41594-022-00874-9 Pubmed: 36482249 ]
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (207 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 27s pre-rrna (guanosine(2922)-2'-o)-methyltransferase (96 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex-associated protein 3 (75 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rrna helicase spb4 (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex protein 2 (81 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (48 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein ytm1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (23 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on ytm1 and spb4. (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
State E1 nucleolar 60S ribosome biogenesis intermediate - Composite model
Resolution: 3.04 Å
EM Method: Single-particle
Fitted PDBs: 7r7a
Q-score: 0.527
Cruz VE, Sekulski K, Peddada N, Sailer C, Balasubramanian S, Weirich CS, Stengel F, Erzberger JP
Nat Struct Mol Biol (2022) 29 pp. 1228-1238 [ DOI: doi:10.1038/s41594-022-00874-9 Pubmed: 36482249 ]
- 60s ribosomal subunit assembly/export protein loc1 (23 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar protein 16 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l6-a (19 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Rrna-processing protein ebp2 (49 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ytm1 isoform 1 (51 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (1 MDa, RNA from Saccharomyces cerevisiae BY4741)
- Pescadillo homolog (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Zinc ion (65 Da, Ligand)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar 60s intermediate purified with tags on ytm1 and spb4. (3 MDa, Complex from Saccharomyces cerevisiae BY4741)
- 5.8s ribosomal rna gene and internal transcribed spacer 2 (27 kDa, RNA from Saccharomyces cerevisiae BY4741)
- Atp-dependent rna helicase has1 (56 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosomal protein (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 27s pre-rrna (guanosine(2922)-2'-o)-methyltransferase (96 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp7 (36 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein brx1 (33 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nuclear gtp-binding protein nug1 (57 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein 15 (25 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex-associated protein 3 (75 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Atp-dependent rrna helicase spb4 (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar complex protein 2 (81 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome biogenesis protein erb1 (91 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Proteasome-interacting protein cic1 (42 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae BY4741)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosome subunit biogenesis protein nip7 (20 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 25s rrna (cytosine(2870)-c(5))-methyltransferase (69 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae BY4741)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae BY4741)
Structure of pre-60S particle bound to DRG1(AFG2)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7z34
Q-score: 0.227
Prattes M, Grishkovskaya I, Hodirnau VV, Hetzmannseder C, Zisser G, Sailer C, Kargas V, Loibl M, Gerhalter M, Kofler L, Warren AJ, Stengel F, Haselbach D, Bergler H
Nat Struct Mol Biol (2022) 29 pp. 942-953 [ Pubmed: 36097293 DOI: doi:10.1038/s41594-022-00832-5 ]
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae S288C)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein rlp24 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Unknown protein (14 kDa, Protein from Saccharomyces cerevisiae S288C ATCC 204508 / S288c)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l12-a (17 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome assembly factor mrt4 (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae S288C)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- Atpase family gene 2 protein (84 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal export protein nmd3 (59 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae S288C)
- Upf0642 protein ybl028c (12 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae S288C)
- 35s pre-ribosomal rna (1 MDa, RNA from Saccharomyces cerevisiae S288C)
- Probable metalloprotease arx1 (65 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae S288C)
- Unknown peptide (1 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae S288C)
- Bud site selection protein 20 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- Nucleolar gtp-binding protein 1 (74 kDa, Protein from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l6-a (20 kDa, Protein from Saccharomyces cerevisiae S288C)
- Pre-60s ribosomal particle in complex with drg1(afg2) (Complex from Saccharomyces cerevisiae S288C)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae S288C)
- Ribosome biogenesis protein nsa2 (29 kDa, Protein from Saccharomyces cerevisiae S288C)
Yeast 80S ribosome stalled on SDD1 mRNA.
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 6snt
Q-score: 0.514
Matsuo Y, Tesina P, Nakajima S, Mizuno M, Endo A, Buschauer R, Cheng J, Shounai O, Ikeuchi K, Saeki Y, Becker T, Beckmann R, Inada T
Nat Struct Mol Biol (2020) 27 pp. 323-332 [ DOI: doi:10.1038/s41594-020-0393-9 Pubmed: 32203490 ]
- 60s ribosomal protein l3 (43 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l22-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l13-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l24-a (17 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s3 (26 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l28 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l43-a (10 kDa, Protein from Saccharomyces cerevisiae)
- Ubiquitin-40s ribosomal protein s31 (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s16-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Guanine nucleotide-binding protein subunit beta-like protein (34 kDa, Protein from Saccharomyces cerevisiae)
- 5s rrna (38 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s18-a (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l37-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s17-b (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l29 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s5 (25 kDa, Protein from Saccharomyces cerevisiae)
- 5.8s rrna (50 kDa, RNA from Saccharomyces cerevisiae)
- Mrna (6 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s23-a (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l38 (8 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s9-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s14-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l4-a (39 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s12 (15 kDa, Protein from Saccharomyces cerevisiae)
- Uncharacterized protein yel057c (27 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l23-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l27-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Trna (24 kDa, RNA from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s19-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l32 (14 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l6-b (18 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l19-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l39 (6 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s7-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l30 (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l5 (33 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l18-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s11-a (17 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s26-a (13 kDa, Protein from Saccharomyces cerevisiae)
- Saccharomyces cerevisiae s288c 25s ribosomal rna (rdn25-1), rrna (1 MDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s15 (16 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l33-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l35-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l31-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l25 (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l42-a (12 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s22-a (14 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s13 (17 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l2-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l15-a (24 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l36-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l8-a (28 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l10 (25 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s1-a (28 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s8-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l26-a (14 kDa, Protein from Saccharomyces cerevisiae)
- L41-a (3 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s21-a (9 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s6-a (27 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l16-a (22 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s30-a (7 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l11-a (19 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s24-a (15 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s10-a (12 kDa, Protein from Saccharomyces cerevisiae)
- Saccharomyces cerevisiae s288c 18s ribosomal rna (rdn18-1), rrna (579 kDa, RNA from Saccharomyces cerevisiae)
- 40s ribosomal protein s29-a (6 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l34-a (13 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l9-a (21 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l7-a (27 kDa, Protein from Saccharomyces cerevisiae)
- Yeast 80s ribosome stalled on sdd1 mrna. (Complex from Saccharomyces cerevisiae)
- 60s ribosomal protein l14-a (15 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l21-a (18 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s25-a (11 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s0-a (28 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s4-a (29 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s2 (27 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l20-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 60s ribosomal protein l17-a (20 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s20 (13 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s28-b (7 kDa, Protein from Saccharomyces cerevisiae)
- 40s ribosomal protein s27-a (8 kDa, Protein from Saccharomyces cerevisiae)