Cryo-EM structure of the plant 80S ribosome
Resolution: 2.2 Å
EM Method: Single-particle
Fitted PDBs: 8b2l
Q-score: 0.639
Smirnova J, Loerke J, Kleinau G, Schmidt A, Burger J, Meyer EH, Mielke T, Scheerer P, Bock R, Spahn CMT, Zoschke R
Nat Plants (2023) 9 pp. 987-1000 [ DOI: doi:10.1038/s41477-023-01407-y Pubmed: 37156858 ]
- 40s ribosomal protein s26 (14 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s25 (11 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l41 (3 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l7a (29 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l34 (13 kDa, Protein from Nicotiana tabacum)
- El31 (60s ribosomal protein l31) (13 kDa, Protein from Nicotiana tabacum)
- El28 (60s ribosomal protein l28) (15 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l39-3 (6 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s24 (15 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s5-like (23 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s9-2-like (23 kDa, Protein from Nicotiana tabacum)
- Ribosomal protein l15 (24 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s27 (9 kDa, Protein from Nicotiana tabacum)
- 50s ribosomal protein l22, chloroplastic (20 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s3a (29 kDa, Protein from Nicotiana tabacum)
- 5s rrna (38 kDa, RNA from Nicotiana tabacum)
- 40s ribosomal protein s16 (16 kDa, Protein from Nicotiana tabacum)
- 18s rrna (583 kDa, RNA from Nicotiana tabacum)
- Ul16 (60s ribosomal protein l10) (25 kDa, Protein from Nicotiana tabacum)
- El6 (60s ribosomal protein l6) (25 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s3-2-like (27 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l13 (23 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s15-like (17 kDa, Protein from Nicotiana tabacum)
- Ul5 (60s ribosomal protein l11) (20 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l38 (8 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l14-1 (15 kDa, Protein from Nicotiana tabacum)
- Ul15 (60s ribosomal protein l27a) (16 kDa, Protein from Nicotiana tabacum)
- Spermine (202 Da, Ligand)
- 40s ribosomal protein s4 (29 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s6 (28 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l44 (12 kDa, Protein from Nicotiana tabacum)
- Zinc ion (65 Da, Ligand)
- Spermidine (145 Da, Ligand)
- 40s ribosomal protein s8 (25 kDa, Protein from Nicotiana tabacum)
- Nascent chain (2 kDa, Protein from Nicotiana tabacum)
- Ribosomal protein l19 (25 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l32-1 (15 kDa, Protein from Nicotiana tabacum)
- El36 (60s ribosomal protein l36) (12 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l24-like (18 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l23a (17 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s20-2 (13 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein sa (35 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s10-1-like (23 kDa, Protein from Nicotiana tabacum)
- Ul2 (60s ribosomal protein l8) (28 kDa, Protein from Nicotiana tabacum)
- Ubiquitin-40s ribosomal protein s27a-like (17 kDa, Protein from Nicotiana tabacum)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l23 (15 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s23 (15 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s28 (7 kDa, Protein from Nicotiana tabacum)
- 25s rrna (1 MDa, RNA from Nicotiana tabacum)
- 40s ribosomal protein s21 (9 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l37a (10 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l29 (7 kDa, Protein from Nicotiana tabacum)
- Water (18 Da, Ligand)
- 60s ribosomal protein l5-like (34 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l30-like (12 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l18-2-like (20 kDa, Protein from Nicotiana tabacum)
- Ul24 (60s ribosomal protein l26) (16 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s30 (6 kDa, Protein from Nicotiana tabacum)
- Guanine nucleotide-binding protein subunit beta-like protein (36 kDa, Protein from Nicotiana tabacum)
- Ul4 (60s ribosomal protein l4) (44 kDa, Protein from Nicotiana tabacum)
- Ul13 (60s ribosomal protein l13a) (23 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l7-4-like (28 kDa, Protein from Nicotiana tabacum)
- Magnesium ion (24 Da, Ligand)
- El33 (60s ribosomal protein l35a) (12 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l18a (21 kDa, Protein from Nicotiana tabacum)
- 30s ribosomal protein s15, chloroplastic (17 kDa, Protein from Nicotiana tabacum)
- Potassium ion (39 Da, Ligand)
- Trna (24 kDa, RNA from Nicotiana tabacum)
- Ul3 (60s ribosomal protein l3) (44 kDa, Protein from Nicotiana tabacum)
- 40s subunit of the cytosolic 80s ribosome from tobacco (Complex from Nicotiana tabacum)
- 40s ribosomal protein s11-like (17 kDa, Protein from Nicotiana tabacum)
- Mrna (3 kDa, RNA from Nicotiana tabacum)
- 60s ribosomal protein l22-2-like (14 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s18 (17 kDa, Protein from Nicotiana tabacum)
- Ul6 (60s ribosomal protein l9) (22 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s19-3 (16 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l21-1-like (18 kDa, Protein from Nicotiana tabacum)
- Ribosomal protein l37 (10 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s14-2 (16 kDa, Protein from Nicotiana tabacum)
- 60s ribosomal protein l35-like (14 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s29 (6 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s2-3-like (30 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s12 (15 kDa, Protein from Nicotiana tabacum)
- 5.8s rrna (52 kDa, RNA from Nicotiana tabacum)
- 40s ribosomal protein s15a-1 (14 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s17-like (16 kDa, Protein from Nicotiana tabacum)
- El27 (60s ribosomal protein l27) (15 kDa, Protein from Nicotiana tabacum)
- 40s ribosomal protein s7 (22 kDa, Protein from Nicotiana tabacum)
Cryo-EM structure of the plant 60S subunit
Resolution: 2.14 Å
EM Method: Single-particle
Fitted PDBs: 8azw
Q-score: 0.675
Smirnova J, Loerke J, Kleinau G, Schmidt A, Burger J, Meyer EH, Mielke T, Scheerer P, Bock R, Spahn CMT, Zoschke R
Nat Plants (2023) 9 pp. 987-1000 [ DOI: doi:10.1038/s41477-023-01407-y Pubmed: 37156858 ]
- Ul5 (60s ribosomal protein l11) (20 kDa, Protein from Nicotiana tabacum)
- El42 (60s ribosomal protein l42) (12 kDa, Protein from Nicotiana tabacum)
- El32 (60s ribosomal protein l32) (15 kDa, Protein from Nicotiana tabacum)
- Magnesium ion (24 Da, Ligand)
- El34 (60s ribosomal protein l34) (13 kDa, Protein from Nicotiana tabacum)
- El31 (60s ribosomal protein l31) (13 kDa, Protein from Nicotiana tabacum)
- 25s rrna (1 MDa, RNA from Nicotiana tabacum)
- El28 (60s ribosomal protein l28) (15 kDa, Protein from Nicotiana tabacum)
- Potassium ion (39 Da, Ligand)
- El8 (60s ribosomal protein l7a) (29 kDa, Protein from Nicotiana tabacum)
- Ul15 (60s ribosomal protein l27a) (16 kDa, Protein from Nicotiana tabacum)
- Ul3 (60s ribosomal protein l3) (44 kDa, Protein from Nicotiana tabacum)
- 40s subunit of the cytosolic 80s ribosome from tobacco (Complex from Nicotiana tabacum)
- Zinc ion (65 Da, Ligand)
- Spermine (202 Da, Ligand)
- El14 (60s ribosomal protein l14) (15 kDa, Protein from Nicotiana tabacum)
- Spermidine (145 Da, Ligand)
- Water (18 Da, Ligand)
- El15 (60s ribosomal protein l15) (24 kDa, Protein from Nicotiana tabacum)
- Ul6 (60s ribosomal protein l9) (22 kDa, Protein from Nicotiana tabacum)
- El18 (60s ribosomal protein l18) (20 kDa, Protein from Nicotiana tabacum)
- El19 (60s ribosomal protein l19) (25 kDa, Protein from Nicotiana tabacum)
- El43 (60s ribosomal protein l37a) (10 kDa, Protein from Nicotiana tabacum)
- El13 (60s ribosomal protein l13) (23 kDa, Protein from Nicotiana tabacum)
- El30 (60s ribosomal protein l30) (12 kDa, Protein from Nicotiana tabacum)
- Ul14 (60s ribosomal protein l23) (15 kDa, Protein from Nicotiana tabacum)
- El24 (60s ribosomal protein l24) (18 kDa, Protein from Nicotiana tabacum)
- Nascent chain (2 kDa, Protein from Nicotiana tabacum)
- El20 (60s ribosomal protein l18a) (21 kDa, Protein from Nicotiana tabacum)
- Ul23 (60s ribosomal protein l23a) (17 kDa, Protein from Nicotiana tabacum)
- Ul22 (60s ribosomal protein l17) (20 kDa, Protein from Nicotiana tabacum)
- El37 (60s ribosomal protein l37) (10 kDa, Protein from Nicotiana tabacum)
- Ul29 (60s ribosomal protein l35) (14 kDa, Protein from Nicotiana tabacum)
- Ul18 (60s ribosomal protein l5) (34 kDa, Protein from Nicotiana tabacum)
- 5s rrna (38 kDa, RNA from Nicotiana tabacum)
- El22 (60s ribosomal protein l22) (14 kDa, Protein from Nicotiana tabacum)
- El36 (60s ribosomal protein l36) (12 kDa, Protein from Nicotiana tabacum)
- 5.8s rrna (52 kDa, RNA from Nicotiana tabacum)
- Ul24 (60s ribosomal protein l26) (16 kDa, Protein from Nicotiana tabacum)
- El38 (60s ribosomal protein l38) (8 kDa, Protein from Nicotiana tabacum)
- El40 (60s ribosomal protein l40) (14 kDa, Protein from Nicotiana tabacum)
- El41 (60s ribosomal protein l41) (3 kDa, Protein from Nicotiana tabacum)
- Ul16 (60s ribosomal protein l10) (25 kDa, Protein from Nicotiana tabacum)
- El27 (60s ribosomal protein l27) (15 kDa, Protein from Nicotiana tabacum)
- El6 (60s ribosomal protein l6) (25 kDa, Protein from Nicotiana tabacum)
- Ul2 (60s ribosomal protein l8) (28 kDa, Protein from Nicotiana tabacum)
- Ul4 (60s ribosomal protein l4) (44 kDa, Protein from Nicotiana tabacum)
- Ul13 (60s ribosomal protein l13a) (23 kDa, Protein from Nicotiana tabacum)
- El39 (60s ribosomal protein l39) (6 kDa, Protein from Nicotiana tabacum)
- El21 (60s ribosomal protein l21) (18 kDa, Protein from Nicotiana tabacum)
- El29 (60s ribosomal protein l29) (7 kDa, Protein from Nicotiana tabacum)
- Ul30 (60s ribosomal protein l7) (28 kDa, Protein from Nicotiana tabacum)
- El33 (60s ribosomal protein l35a) (12 kDa, Protein from Nicotiana tabacum)