Cryo-EM structure of Thogoto virus polymerase in a transcription elongation-reception conformation
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8z9h
Q-score: 0.514
Xue L, Chang T, Li Z, Wang C, Zhao H, Li M, Tang P, Wen X, Yu M, Wu J, Bao X, Wang X, Gong P, He J, Chen X, Xiong X
Nat Commun (2024) 15 pp. 4620-4620 [ DOI: doi:10.1038/s41467-024-48848-3 Pubmed: 38816392 ]
- 7-methyl-guanosine-5'-triphosphate-5'-(2'-o-methyl)-adenosine (801 Da, Ligand)
- Rna (5'-r(p*gp*cp*ap*ap*ap*ap*ap*cp*a)-3') (2 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Rna-directed rna polymerase catalytic subunit (81 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Polymerase acidic protein (71 kDa, Protein from Thogoto virus (isolate SiAr 126))
- Cryo-em structure of thogoto virus polymerase in a transcription elongation-reception conformation (Complex from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*ap*gp*ap*gp*ap*ap*ap*up*cp*ap*ap*gp*gp*cp*ap*gp*up*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Rna (5'-r(*gp*ap*cp*up*gp*cp*cp*up*gp*up*up*up*up*up*gp*cp*u)-3') (5 kDa, RNA from Thogoto virus (isolate SiAr 126))
- Polymerase basic protein 2 (94 kDa, Protein from Thogoto virus (isolate SiAr 126))
CryoEM structure of the Chikungunya virus nsP1 capping pores in complex with m7GTP and SAH ligands
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8aow
Q-score: 0.584
Jones R, Hons M, Rabah N, Zamarreno N, Arranz R, Reguera J
PNAS (2023) 120 pp. e2213934120-e2213934120 [ Pubmed: 36913573 DOI: doi:10.1073/pnas.2213934120 ]
- Zinc ion (65 Da, Ligand)
- Chikv nsp1 capping pores in complex with m7gtp and sah ligand with magnesium cofactor. (720 kDa, Complex from Chikungunya virus strain S27-African prototype)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- 7-methyl-guanosine-5'-triphosphate (538 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Mrna-capping enzyme nsp1 (60 kDa, Protein from Chikungunya virus strain S27-African prototype)
- Water (18 Da, Ligand)
Cryo-EM Structure of Chikungunya Virus Nonstructural Protein 1 with m7GTP
Resolution: 2.19 Å
EM Method: Single-particle
Fitted PDBs: 7fgg
Q-score: 0.698
Zhang K, Law MCY, Nguyen TM, Tan YB, Wirawan M, Law YS, Jeong LS, Luo D
Cell Rep (2022) 40 pp. 111133-111133 [ DOI: doi:10.1016/j.celrep.2022.111133 Pubmed: 35905713 ]
- Zinc ion (65 Da, Ligand)
- Nonstructural protein 1 (Complex from Chikungunya virus strain S27-African prototype)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Mrna-capping enzyme nsp1 (62 kDa, Protein from Chikungunya virus strain S27-African prototype)
- 7-methyl-guanosine-5'-triphosphate (538 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Water (18 Da, Ligand)