Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8v9r
Q-score: 0.475
Ghanbarpour A, Sauer RT, Davis JH
To Be Published
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli)
- Dihydrofolate reductase (23 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Methotrexate (454 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Clpx.clpp.dhfr (Complex from Escherichia coli)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 9c88
Q-score: 0.5
Ghanbarpour A, Sauer RT, Davis JH
To Be Published
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli)
- Branched-degron dhfr (869 Da, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Clpx.clpp.dhfr (Complex from Escherichia coli)
Cryo-EM structure of the ClpP degradation system in Streptomyces hawaiiensis
Resolution: 2.34 Å
EM Method: Single-particle
Fitted PDBs: 8xn4
Q-score: 0.56
Cryo-EM structure of ClpP1P2 in complex with ADEP1 from Streptomyces hawaiiensis
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8xop
Q-score: 0.498
Xu X, Wang Y, Huang W, Li D, Deng Z, Long F
Mbio (2024) 15 pp. e0003124-e0003124 [ Pubmed: 38501868 DOI: doi:10.1128/mbio.00031-24 ]
- Adep1 (736 Da, Protein from synthetic construct)
- Atp-dependent clp protease proteolytic subunit (24 kDa, Protein from Streptomyces hawaiiensis)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Streptomyces hawaiiensis)
- Clpp1,clpp2 (Complex from Streptomyces hawaiiensis)
Cryo-EM structure of substrate-free ClpX.ClpP
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 8e91
Q-score: 0.517
Ghanbarpour A, Cohen S, Davis JH, Sauer RT
Nat Commun (2023) [ ]
- Clpxp aaa protease (Complex from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (46 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
ClpP and ClpX IGF loop in ClpX-ClpP complex bound to ssrA tagged GFP
Resolution: 2.88 Å
EM Method: Single-particle
Fitted PDBs: 6wr2
Q-score: 0.518
Fei X, Bell TA, Barkow SR, Baker TA, Sauer RT
eLife (2020) 9 [ DOI: doi:10.7554/eLife.61496 Pubmed: 33089779 ]
- Clpx-clpp-gfpssra-atp/atprs (Complex from Escherichia coli)
- Water (18 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli (strain K12))
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli (strain K12))
ClpAP Engaged1 State bound to RepA-GFP
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 6w1z
Q-score: 0.473
Lopez KE, Rizo AN, Tse E, Lin J, Scull NW, Thwin AC, Lucius AL, Shorter J, Southworth DR
Nat Struct Mol Biol (2020) 27 pp. 406-416 [ DOI: doi:10.1038/s41594-020-0409-5 Pubmed: 32313240 ]
- Clpap (840 kDa, Complex)
- Atp-dependent clp protease proteolytic subunit (Complex from Escherichia coli)
- Repa, green fluorescent protein fusion (Complex from synthetic construct)
- Atp-dependent clp protease atp-binding subunit clpa (Complex from Escherichia coli K-12)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease atp-binding subunit clpa (84 kDa, Protein from Escherichia coli (strain K12))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Repa, green fluorescent protein fusion (2 kDa, Protein from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
ClpXP from Neisseria meningitidis - Conformation B
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6vfx
Q-score: 0.413
Ripstein ZA, Vahidi S, Houry WA, Rubinstein JL, Kay LE
eLife (2020) 9 [ DOI: doi:10.7554/eLife.52158 Pubmed: 31916936 ]
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (45 kDa, Protein from Neisseria meningitidis)
- Atp-dependent clp protease atp-binding subunit clpx, atp-dependent clp protease proteolytic subunit (Complex from Neisseria meningitidis)
- Unidentified peptide substrate (Complex from Escherichia coli)
- Clpxp complex (860 kDa, Complex)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Neisseria meningitidis)
- Unidentified peptide substrate (613 Da, Protein from Escherichia coli BL21(DE3))