Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8v9r
Q-score: 0.475
Ghanbarpour A, Sauer RT, Davis JH
To Be Published
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli)
- Dihydrofolate reductase (23 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Methotrexate (454 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Clpx.clpp.dhfr (Complex from Escherichia coli)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 9c87
Q-score: 0.335
Ghanbarpour A, Sauer RT, Davis JH
To Be Published
- Clpx.clpp.linear-degron dhfr-ssra (Complex from Escherichia coli)
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Methotrexate (454 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dihydrofolate reductase (23 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 9c88
Q-score: 0.5
Ghanbarpour A, Sauer RT, Davis JH
To Be Published
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli)
- Branched-degron dhfr (869 Da, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Clpx.clpp.dhfr (Complex from Escherichia coli)
Cryo-EM structure of the ClpC1:ClpP1P2 degradation complex in Streptomyces hawaiiensis
Resolution: 1.96 Å
EM Method: Single-particle
Fitted PDBs: 8xon
Q-score: 0.503
Xu X, Wang Y, Huang W, Li D, Deng Z, Long F
Mbio (2024) 15 pp. e0003124-e0003124 [ Pubmed: 38501868 DOI: doi:10.1128/mbio.00031-24 ]
- Ndp-hexose 4-ketoreductase (77 kDa, Protein from Streptomyces hawaiiensis)
- Clpp1,clpp2,clpc1,casein (Complex from Streptomyces hawaiiensis)
- Atp-dependent clp protease proteolytic subunit (24 kDa, Protein from Streptomyces hawaiiensis)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Streptomyces hawaiiensis)
- Casein (2 kDa, Protein from Bos taurus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of the ClpC1:ClpP1P2 degradation complex in Streptomyces hawaiiensis
Resolution: 1.84 Å
EM Method: Single-particle
Fitted PDBs: 8xoo
Q-score: 0.514
Xu X, Wang Y, Huang W, Li D, Deng Z, Long F
Mbio (2024) 15 pp. e0003124-e0003124 [ Pubmed: 38501868 DOI: doi:10.1128/mbio.00031-24 ]
- Ndp-hexose 4-ketoreductase (77 kDa, Protein from Streptomyces hawaiiensis)
- Clpp1,clpp2,clpc1,casein (Complex from Streptomyces hawaiiensis)
- Atp-dependent clp protease proteolytic subunit (24 kDa, Protein from Streptomyces hawaiiensis)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Streptomyces hawaiiensis)
- Casein (2 kDa, Protein from Bos taurus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of substrate-free DNClpX.ClpP from singly capped particles
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8e7v
Q-score: 0.468
Ghanbarpour A, Cohen S, Davis JH, Sauer RT
Nat Commun (2023) [ ]
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli)
- Clpxp aaa protease (Complex from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of substrate-free DNClpX.ClpP
Resolution: 3.12 Å
EM Method: Single-particle
Fitted PDBs: 8e8q
Q-score: 0.452
Ghanbarpour A, Cohen S, Davis JH, Sauer RT
Nat Commun (2023) [ ]
- Atp-dependent clp protease atp-binding subunit clpx (42 kDa, Protein from Escherichia coli)
- Clpxp aaa + protease (Complex from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Cryo-EM structure of substrate-free ClpX.ClpP
Resolution: 2.57 Å
EM Method: Single-particle
Fitted PDBs: 8e91
Q-score: 0.517
Ghanbarpour A, Cohen S, Davis JH, Sauer RT
Nat Commun (2023) [ ]
- Clpxp aaa protease (Complex from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (46 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8et3
Q-score: 0.382
Ghanbarpour A, Fei X, Baker TA, Davis JH, Sauer RT
PNAS (2023) 120 pp. e2219044120-e2219044120 [ DOI: doi:10.1073/pnas.2219044120 Pubmed: 36730206 ]
- Stringent starvation protein b (18 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Atp-dependent clp protease atp-binding subunit clpx (46 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Green fluorescent protein (30 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (23 kDa, Protein from Escherichia coli)
- Clpxp aaa protease complex bound with sspb and gfp-ssra (Complex from Escherichia coli)
ClpAP complex bound to ClpS N-terminal extension, class IIa
Resolution: 3.38 Å
EM Method: Single-particle
Fitted PDBs: 7uiv
Q-score: 0.416
Kim S, Fei X, Sauer RT, Baker TA
Nat Struct Mol Biol (2022) 29 pp. 1068-1079 [ DOI: doi:10.1038/s41594-022-00850-3 Pubmed: 36329286 ]
- Atp-dependent clp protease atp-binding subunit clpa (84 kDa, Protein from Escherichia coli)
- Atp-dependent clp protease proteolytic subunit (22 kDa, Protein from Escherichia coli)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Atp-dependent clp protease adapter protein clps (12 kDa, Protein from Escherichia coli)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Aaa+ protease complex clpap bound to adaptor protein clps and gfp (Complex from Escherichia coli)