CryoEM Structure INO80core Hexasome complex composite map state1
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8oo7
Q-score: 0.556
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Histones (Complex from Homo sapiens)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Dna (Complex from Synthetic construct)
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
INO80 core bound to hexasome composite map of state 2
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8oop
Q-score: 0.554
Zhang M, Jungblut A, Kunert F, Hauptmann L, Hoffmann T, Kolesnikova O, Metzner F, Moldt M, Weis F, DiMaio F, Hopfner KP, Eustermann S
Science (2023) 381 pp. 313-319 [ DOI: doi:10.1126/science.adf6287 Pubmed: 37384673 ]
- Chromatin-remodeling atpase ino80 (130 kDa, Protein from Thermochaetoides thermophila)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Chromatin-remodeling complex subunit ies6 (23 kDa, Protein from Thermochaetoides thermophila)
- Histones (Complex from Homo sapiens)
- Histone h2b (13 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Histone h2a (14 kDa, Protein from Homo sapiens)
- Dna strand 1 (69 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Tetrafluoroaluminate ion (102 Da, Ligand)
- Ino eighty subunit 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Ino80 core module in complex with hexasome (861 kDa, Complex)
- Ruvb-like protein 2 (53 kDa, Protein from Thermochaetoides thermophila)
- Dna (Complex from Synthetic construct)
- Chromatin remodeler ino80 (Complex from Thermochaetoides thermophila)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 5 (87 kDa, Protein from Thermochaetoides thermophila)
- Dna strand 2 (70 kDa, DNA from synthetic construct)
- Ruvb-like protein 1 (50 kDa, Protein from Thermochaetoides thermophila)
Cryo-EM structure of the human INO80 complex bound to nucleosome
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 6hts
Q-score: 0.181
Ayala R, Willhoft O, Aramayo RJ, Wilkinson M, McCormack EA, Ocloo L, Wigley DB, Zhang X
Nature (2018) 556 pp. 391-395 [ DOI: doi:10.1038/s41586-018-0021-6 Pubmed: 29643506 ]
- Human ino80 (Complex from synthetic construct)
- Ino80 complex subunit b (38 kDa, Protein from synthetic construct)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone subunits (Complex from Homo sapiens)
- Dna (142-mer) (62 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna (142-mer) (61 kDa, DNA from synthetic construct)
- Nucleosome (Complex from Homo sapiens)
- Actin-related protein 5 (68 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Ruvb-like 1 (50 kDa, Protein from Homo sapiens)
- Complex of core human ino80 with nucleosome (Complex from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Dna helicase ino80 (146 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Ruvb-like 2 (51 kDa, Protein from Homo sapiens)