Cryo-EM structure of the human INO80 complex bound to a WT nucleosome
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7zi4
Q-score: 0.462
Vance NR, Ayala R, Willhoft O, Tvardovskiy A, McCormack EA, Bartke T, Zhang X, Wigley DB
To Be Published
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Dna (158-mer) (48 kDa, DNA from synthetic construct)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Actin-related protein 5 (68 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Ino80 complex subunit c (20 kDa, Protein from Homo sapiens)
- Ruvb-like 2 (51 kDa, Protein from Homo sapiens)
- Ino80 complex subunit b (38 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Dna (158-mer) (48 kDa, DNA from synthetic construct)
- Ruvb-like 1 (50 kDa, Protein from Homo sapiens)
- Chromatin-remodeling atpase ino80 (177 kDa, Protein from Homo sapiens)
- Complex of human ino80core bound to a wt nucleosome (1 MDa, Complex from Homo sapiens)
Cryo-EM structure of the human INO80 complex bound to nucleosome
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 6hts
Q-score: 0.181
Ayala R, Willhoft O, Aramayo RJ, Wilkinson M, McCormack EA, Ocloo L, Wigley DB, Zhang X
Nature (2018) 556 pp. 391-395 [ DOI: doi:10.1038/s41586-018-0021-6 Pubmed: 29643506 ]
- Human ino80 (Complex from synthetic construct)
- Ino80 complex subunit b (38 kDa, Protein from synthetic construct)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Histone subunits (Complex from Homo sapiens)
- Dna (142-mer) (62 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Dna (142-mer) (61 kDa, DNA from synthetic construct)
- Nucleosome (Complex from Homo sapiens)
- Actin-related protein 5 (68 kDa, Protein from Homo sapiens)
- Histone h3.1 (15 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Ruvb-like 1 (50 kDa, Protein from Homo sapiens)
- Complex of core human ino80 with nucleosome (Complex from Homo sapiens)
- Histone h2b type 1-j (13 kDa, Protein from Homo sapiens)
- Dna helicase ino80 (146 kDa, Protein from Homo sapiens)
- Histone h2a type 1-b/e (14 kDa, Protein from Homo sapiens)
- Ruvb-like 2 (51 kDa, Protein from Homo sapiens)
CryoEM Structure INO80core Nucleosome complex
Resolution: 4.34 Å
EM Method: Single-particle
Fitted PDBs: 6fml
Q-score: 0.33
Eustermann S, Schall K, Kostrewa D, Lakomek K, Strauss M, Moldt M, Hopfner KP
Nature (2018) 556 pp. 386-390 [ Pubmed: 29643509 DOI: doi:10.1038/s41586-018-0029-y ]
- Histone octamer (Complex from Homo sapiens)
- Ino80core (Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
- Ino80 (210 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Ino80core nucleosome complex (1 MDa, Complex)
- Nucleosomal dna strand 2 (60 kDa, DNA from synthetic construct)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Histone h3.2 (15 kDa, Protein from Homo sapiens)
- Ruvb-like helicase (50 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Ies6 (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Actin related protein 5 (85 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Histone h2b type 1-c/e/f/g/i (13 kDa, Protein from Homo sapiens)
- Synthetic deoxyribonucleic acid (Complex from synthetic construct)
- Histone h2a type 1 (13 kDa, Protein from Homo sapiens)
- Histone h4 (11 kDa, Protein from Homo sapiens)
- Ruvb-like helicase (53 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nucleosomal dna strand 1 (60 kDa, DNA from synthetic construct)
- Les2 (52 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))