Single-particle Cryo-EM structure of Arp2/3 complex at branched-actin junction.
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7tpt
Q-score: 0.266
Ding B, Narvaez-Ortiz HY, Singh Y, Hocky GM, Chowdhury S, Nolen BJ
PNAS (2022) 119 pp. e2202723119-e2202723119 [ Pubmed: 35622886 DOI: doi:10.1073/pnas.2202723119 ]
- Actin-related protein 2 (44 kDa, Protein from cattle)
- Actin-related protein 2/3 complex subunit 2 (34 kDa, Protein from cattle)
- Magnesium ion (24 Da, Ligand)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from cattle)
- Phalloidin (808 Da, Protein from Amanita phalloides)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from cattle)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-related protein 2/3 complex subunit 1b (41 kDa, Protein from cattle)
- Complex consisting of arp2/3 complex-mediated branched actin junction (834 kDa, Complex from Bos taurus)
- Actin, alpha skeletal muscle (42 kDa, Protein from rabbit)
- Actin-related protein 2/3 complex subunit 3 (20 kDa, Protein from cattle)
- Actin-related protein 3 (47 kDa, Protein from cattle)
Structure of Dip1-activated Arp2/3 complex with nucleated actin filament
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 6w17
Q-score: 0.409
Shaaban M, Chowdhury S, Nolen BJ
Nat Struct Mol Biol (2020) 27 pp. 1009-1016 [ Pubmed: 32839613 DOI: doi:10.1038/s41594-020-0481-x ]
- Magnesium ion (24 Da, Ligand)
- Complex consisting of actin-filament nucleator, arp2/3 complex associated with nucleation promoting factor dip1 and first four actin subunits from the pointed end of the nucleated actin-filament (446 kDa, Complex from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from Fission yeast)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Fission yeast)
- Protein dip1 (43 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin, alpha skeletal muscle (42 kDa, Protein from Rabbit)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 3 (47 kDa, Protein from Fission yeast)
- Phalloidin (808 Da, Protein from death cap)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Fission yeast)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Fission yeast)
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from Fission yeast)
- Actin-related protein 2 (44 kDa, Protein from Fission yeast)
Structure of S. pombe Arp2/3 complex in inactive state
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6w18
Q-score: 0.311
Shaaban M, Chowdhury S, Nolen BJ
Nat Struct Mol Biol (2020) 27 pp. 1009-1016 [ Pubmed: 32839613 DOI: doi:10.1038/s41594-020-0481-x ]
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Magnesium ion (24 Da, Ligand)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin-related protein 3 (47 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Arp2/3 complex (225 kDa, Complex from Schizosaccharomyces pombe (strain 972 / ATCC 24843))
- Actin-related protein 2 (44 kDa, Protein from Schizosaccharomyces pombe (strain 972 / ATCC 24843))