Structure of Arp4-Ies4-N-actin-Arp8-Ino80HSA subcomplex (A-module) of Chaetomium thermophilum INO80
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8a5d
Q-score: 0.572
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Magnesium ion (24 Da, Ligand)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Ino80 atpase (37 kDa, Protein from Thermochaetoides thermophila)
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Chaetomium thermophilum ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Thermochaetoides thermophila)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8a5p
Q-score: 0.415
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Dna (36-mer) (11 kDa, DNA from synthetic construct)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Ino80 atpase (210 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) on curved dna (Complex from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
- Dna (36-mer) (11 kDa, DNA from synthetic construct)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8a5q
Q-score: 0.483
Kunert F, Metzner FJ, Jung J, Hopfler M, Woike S, Schall K, Kostrewa D, Moldt M, Chen JX, Bantele S, Pfander B, Eustermann S, Hopfner KP
Sci Adv (2022) 8 pp. eadd3189-eadd3189 [ Pubmed: 36490333 DOI: doi:10.1126/sciadv.add3189 ]
- Dna (26-mer) (69 kDa, DNA from synthetic construct)
- Dna (26-mer) (70 kDa, DNA from synthetic construct)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin related protein 4 (arp4) (51 kDa, Protein from Thermochaetoides thermophila)
- Magnesium ion (24 Da, Ligand)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) on straight dna (Complex)
- Ino80 a-module (arp4-ies4-n-actin-arp8-ino80hsa) (Complex from Thermochaetoides thermophila)
- Ino80 atpase (210 kDa, Protein from Thermochaetoides thermophila)
- Actin-related protein 8 (84 kDa, Protein from Thermochaetoides thermophila)
- Actin (41 kDa, Protein from Thermochaetoides thermophila)
- Dna (Complex from synthetic construct)
- Ies4 (29 kDa, Protein from Thermochaetoides thermophila)
Negative stain microscopy of a dimer of Actin-related protein 8 (Arp8) from S. cerevisiae.
Saravanan M, Wuerges J, Bose D, McCormack EA, Cook NJ, Zhang X, Wigley DB
PNAS (2012) 109 pp. 20883-20888 [ Pubmed: 23213201 DOI: doi:10.1073/pnas.1214735109 ]
Saravanan M, Wuerges J, Bose D, McCormack EA, Cook NJ, Zhang X, Wigley DB
PNAS (2012) 109 pp. 20883-20888 [ Pubmed: 23213201 DOI: doi:10.1073/pnas.1214735109 ]