Cryo-EM structure of ADGRF1 coupled to miniGs/q
Resolution: 3.44 Å
EM Method: Single-particle
Fitted PDBs: 8g2y
Q-score: 0.47
Jones DTD, Dates AN, Rawson SD, Burruss MM, Lipper CH, Blacklow SC
Nat Commun (2023) 14 pp. 2490-2490 [ Pubmed: 37120430 DOI: doi:10.1038/s41467-023-38083-7 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (39 kDa, Protein from Homo sapiens)
- Adhesion g-protein-coupled receptor f1 (45 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Tethered agonist activated adhesion gpcr adgrf1 coupled to minigs/q (Complex from Homo sapiens)
- Minig alpha s/q chimera (47 kDa, Protein from Homo sapiens)
- [1-myristoyl-glycerol-3-yl]phosphonylcholine (468 Da, Ligand)
- Nanobody 35 (19 kDa, Protein from Lama glama)
Resolution: 2.85 Å
EM Method: Single-particle
Fitted PDBs: 7wxu
Q-score: 0.533
Zhu X, Qian Y, Li X, Xu Z, Xia R, Wang N, Liang J, Yin H, Zhang A, Guo C, Wang G, He Y
Nat Commun (2022) 13 pp. 5513-5513 [ DOI: doi:10.1038/s41467-022-33173-4 Pubmed: 36127364 ]
- Nb35 (17 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nb35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor f1 (101 kDa, Protein from Homo sapiens)
- Gpcr/g-protein (Complex from Lama glama)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Engineered mini galpha-q subunit (41 kDa, Protein from Homo sapiens)
Resolution: 2.84 Å
EM Method: Single-particle
Fitted PDBs: 7wxw
Q-score: 0.503
Zhu X, Qian Y, Li X, Xu Z, Xia R, Wang N, Liang J, Yin H, Zhang A, Guo C, Wang G, He Y
Nat Commun (2022) 13 pp. 5513-5513 [ DOI: doi:10.1038/s41467-022-33173-4 Pubmed: 36127364 ]
- Nb35 (17 kDa, Protein from Lama glama)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Nb35 (Complex)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor f1 (101 kDa, Protein from Homo sapiens)
- Gpcr/g-protein (Complex from Lama glama)
- Gpcr/g-protein complex (Complex from Homo sapiens)
- Engineered mini galpha-s subunit (41 kDa, Protein from Homo sapiens)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7wy0
Q-score: 0.45
Zhu X, Qian Y, Li X, Xu Z, Xia R, Wang N, Liang J, Yin H, Zhang A, Guo C, Wang G, He Y
Nat Commun (2022) 13 pp. 5513-5513 [ DOI: doi:10.1038/s41467-022-33173-4 Pubmed: 36127364 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor f1 (101 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Engineered g alpha 13 subunit (41 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7wz7
Q-score: 0.399
Zhu X, Qian Y, Li X, Xu Z, Xia R, Wang N, Liang J, Yin H, Zhang A, Guo C, Wang G, He Y
Nat Commun (2022) 13 pp. 5513-5513 [ DOI: doi:10.1038/s41467-022-33173-4 Pubmed: 36127364 ]
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Engineered g alpha 12 subunit (39 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor f1 (101 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
Resolution: 3.09 Å
EM Method: Single-particle
Fitted PDBs: 7x2v
Q-score: 0.316
Zhu X, Qian Y, Li X, Xu Z, Xia R, Wang N, Liang J, Yin H, Zhang A, Guo C, Wang G, He Y
Nat Commun (2022) 13 pp. 5513-5513 [ DOI: doi:10.1038/s41467-022-33173-4 Pubmed: 36127364 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (40 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (37 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor f1 (101 kDa, Protein from Homo sapiens)
- Scfv16 (26 kDa, Protein from Homo sapiens)
- Gpcr/g-protein complex (Complex from Homo sapiens)
Cryo-EM structure of the adhesion GPCR ADGRF1 in complex with miniGs
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7wu3
Q-score: 0.501
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
Nature (2022) 604 pp. 779-785 [ DOI: doi:10.1038/s41586-022-04580-w Pubmed: 35418679 ]
- Nanobody nb35 (17 kDa, Protein from Lama glama)
- Nanobody nb35 (Complex from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(s) subunit alpha isoforms short (28 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- Adhesion g-protein coupled receptor f1 (74 kDa, Protein from Homo sapiens)
- [(2~{r})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] hexadecanoate (496 Da, Ligand)
- The adhesion gpcr adgrf1 in complex with minigs (Complex from Homo sapiens)
Cryo-EM structure of the adhesion GPCR ADGRF1 in complex with miniGi
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7wu4
Q-score: 0.428
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
Nature (2022) 604 pp. 779-785 [ DOI: doi:10.1038/s41586-022-04580-w Pubmed: 35418679 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- The adhesion gpcr adgrf1 in complex with minigi (Complex from Homo sapiens)
- Adhesion g-protein coupled receptor f1 (74 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- [(2~{r})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] hexadecanoate (496 Da, Ligand)
- Cholesterol (386 Da, Ligand)
Cryo-EM structure of the adhesion GPCR ADGRF1(H565A/T567A) in complex with miniGi
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7wu5
Q-score: 0.492
Qu X, Qiu N, Wang M, Zhang B, Du J, Zhong Z, Xu W, Chu X, Ma L, Yi C, Han S, Shui W, Zhao Q, Wu B
Nature (2022) 604 pp. 779-785 [ DOI: doi:10.1038/s41586-022-04580-w Pubmed: 35418679 ]
- Guanine nucleotide-binding protein g(i) subunit alpha-1 (27 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2 (7 kDa, Protein from Homo sapiens)
- Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1 (38 kDa, Protein from Homo sapiens)
- The adhesion gpcr adgrf1(h565a/t567a) in complex with minigi (Complex from Homo sapiens)
- [(2~{r})-2-oxidanyl-3-[oxidanyl-[2-(trimethyl-$l^{4}-azanyl)ethoxy]phosphoryl]oxy-propyl] hexadecanoate (496 Da, Ligand)
- Adhesion g-protein coupled receptor f1 (74 kDa, Protein from Homo sapiens)
- Cholesterol (386 Da, Ligand)