Structure of a gp140 SpyTag-SpyCatcher mi3 nanoparticle including mi3 density only.
Resolution: 5.3 Å
EM Method: Single-particle
Fitted PDBs: 9fo3
Q-score: 0.231
Malebo K, Woodward J, Ximba P, Mkhize Q, Cingo S, Moyo-Gwete T, Moore PL, Williamson AL, Chapman R
Vaccines (Basel) (2024) 12 [ DOI: doi:10.3390/vaccines12091063 Pubmed: 39340093 ]
- Cap255 gp140 spytag-spycatcher mi3 nanoparticle (4 MDa, Complex from Thermotoga maritima)
- Cap255 gp140 spytag-spycatcher mi3,2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase,2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (33 kDa, Protein from Thermotoga maritima)
Rabbit muscle aldolase at 3A resolution imaged at 0.55A/pix
Karia D, Koh A, Yang W, Cushing V, van der Ploeg R, Basanta B, Mihaylov D, Khavnekar S, Vyroubal O, Malinsky M, Shanel O, Dolezal V, Plitzko JM, Yu L, Lander GC, Aricescu R, Greber B, Kotecha A
To Be Published
Rabbit muscle aldolase at 2.8A imaged at 0.7Apix
Karia D, Koh A, Yang W, Cushing V, van der Ploeg R, Basanta B, Mihaylov D, Khavnekar S, Vyroubal O, Malinsky M, Shanel O, Dolezal V, Plitzko JM, Yu L, Lander GC, Aricescu R, Greber B, Kotecha A
To Be Published
Lassa GPC (strain Josiah) bound to rabbit polyclonal base-targeting antibody Base-1
Brouwer PJM, Perrett HR, Nijhuis H, Lee W-H, Burger J, Lloyd NM, Himansu S, Beaumont T, Carfi A, Bukreyev A, Sanders RW, van Gils MJ, Ward AB
To Be Published
- Polyclonal antibody base-1 heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal base-targeting antibody base-1 (Complex)
- Glycoprotein g1 (29 kDa, Protein from Lassa virus)
- Glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus)
- Polyclonal antibody base-1 heavy chain, polyclonal antibody base-1 light chain (Complex from Oryctolagus cuniculus)
- Polyclonal antibody base-1 light chain (9 kDa, Protein from Oryctolagus cuniculus)
Lassa GPC (strain Josiah) bound to rabbit polyclonal fusion-peptide-targeting antibody FP-1
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8tye
Q-score: 0.421
Brouwer PJM, Perrett HR, Beaumont T, Nijhuis H, Kruijer S, Burger JA, Bontjer I, Lee WH, Ferguson JA, Schauflinger M, Muller-Krauter H, Sanders RW, Strecker T, van Gils MJ, Ward AB
Cell Rep (2024) 43 pp. 114708-114708 [ Pubmed: 39243373 DOI: doi:10.1016/j.celrep.2024.114708 ]
- Polyclonal fv heavy chain (9 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein gp1 (29 kDa, Protein from Lassa virus (strain Mouse/Sierra Leone/Josiah/1976))
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Polyclonal fv light chain (8 kDa, Protein from Oryctolagus cuniculus)
- Glycoprotein gp2 (44 kDa, Protein from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8))
- Polyclonal antibody fp-1 heavy chain, polyclonal antibody fp-1 light chain (Complex from Oryctolagus cuniculus)
- Heterotrimeric lassa gpc bound to heterodimeric rabbit polyclonal fusion-peptide-targeting antibody fp-1 (Complex)
- Glycoprotein g1, glycoprotein g2, 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase fusion protein (Complex from Lassa virus Josiah)
Cryo-EM structure of Aldolase collected by EPU on Glacios at 2.6 Angstrom resolution
Jia L, Ruben EA, Olsen SK, Wasmuth EV
To Be Published
- Water (18 Da, Ligand)
- Aldolase (Complex from Oryctolagus cuniculus)
- Fructose-bisphosphate aldolase a (37 kDa, Protein from Oryctolagus cuniculus)
Cryo-EM structure of Aldolase collected by SerialEM on Glacios at 2.7 Angstrom resolution
Jia L, Ruben EA, Olsen SK, Wasmuth EV
To Be Published
- Water (18 Da, Ligand)
- Aldolase (Complex from Oryctolagus cuniculus)
- Fructose-bisphosphate aldolase a (37 kDa, Protein from Oryctolagus cuniculus)
Cryo-EM structure of Aldolase collected by Leginon on Glacios at 2.6 Angstrom resolution
Jia L, Ruben EA, Olsen SK, Wasmuth EV
To Be Published
- Water (18 Da, Ligand)
- Aldolase (Complex from Oryctolagus cuniculus)
- Fructose-bisphosphate aldolase a (37 kDa, Protein from Oryctolagus cuniculus)
Aldolase at 100 keV on the Alpine detector with a side-entry cryoholder
Ghanaian virus fusion glycoprotein (GhV F)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8tvb
Q-score: 0.56
Wang Z, McCallum M, Yan L, Gibson CA, Sharkey W, Park YJ, Dang HV, Amaya M, Person A, Broder CC, Veesler D
PNAS (2024) 121 pp. e2314990121-e2314990121 [ DOI: doi:10.1073/pnas.2314990121 Pubmed: 38593070 ]
- Fusion glycoprotein f0,2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (96 kDa, Protein from Thermotoga maritima MSB8)
- 2-acetamido-2-deoxy-beta-d-glucopyranose (221 Da, Ligand)
- Prefusion ghanian henipavirus f fused to the i53-50a trimer via an intervening 16 gs linker (Complex from Henipavirus ghanaense)