CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, pre-state
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 6oax
Q-score: 0.5
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Hyperactive clpb mutant k476c (Complex from Escherichia coli K-12)
CryoEM map of the hyperactive ClpB mutant K476C, bound to casein, post-state
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 6oay
Q-score: 0.437
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Hyperactive disaggregase clpb (97 kDa, Protein from Escherichia coli K-12)
- Alpha-s1-casein (Complex from Bos taurus)
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Hyperactive clpb mutant k476c (Complex from Escherichia coli K-12)
CryoEM focus classification map of the hyperactive ClpB mutant K476C, bound to casein, NTD-trimer
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 6og3
Q-score: 0.306
Rizo AN, Lin J, Gates SN, Tse E, Bart SM, Castellano LM, DiMaio F, Shorter J, Southworth DR
Nat Commun (2019) 10 pp. 2393-2393 [ Pubmed: 31160557 DOI: doi:10.1038/s41467-019-10150-y ]
- Hyperactive disaggregase clpb (96 kDa, Protein from Escherichia coli K-12)
- Hyperactive clpb mutant k476c bound to casein (Complex)
- Alpha-s1-casein (Complex from Bos taurus)
- Hyperactive clpb mutant k476c_ntd trimer (Complex from Escherichia coli K-12)
- Alpha s1-casein (783 Da, Protein from Bos taurus)
S. cerevisiae Hsp104:casein complex, Middle Domain Conformation
Resolution: 6.7 Å
EM Method: Single-particle
Fitted PDBs: 5vy9
Q-score: 0.161
Gates SN, Yokom AL, Lin J, Jackrel ME, Rizo AN, Kendsersky NM, Buell CE, Sweeny EA, Mack KL, Chuang E, Torrente MP, Su M, Shorter J, Southworth DR
Science (2017) 357 pp. 273-279 [ Pubmed: 28619716 DOI: doi:10.1126/science.aan1052 ]
- Heat shock protein 104 (102 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- S. cerevisiae hsp104:casein complex, middle domain conformation (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Casein (Complex from Bos taurus)
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- Hsp104 (Complex)
S. cerevisiae Hsp104:casein complex, Extended Conformation
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 5vya
Q-score: 0.313
Gates SN, Yokom AL, Lin J, Jackrel ME, Rizo AN, Kendsersky NM, Buell CE, Sweeny EA, Mack KL, Chuang E, Torrente MP, Su M, Shorter J, Southworth DR
Science (2017) 357 pp. 273-279 [ DOI: doi:10.1126/science.aan1052 Pubmed: 28619716 ]
- Heat shock protein 104 (102 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Phosphothiophosphoric acid-adenylate ester (523 Da, Ligand)
- S. cerevisiae hsp104:casein complex, extended conformation (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Alpha-s1-casein (2 kDa, Protein from Bos taurus)