Microtubule-associated Kif5B IAK tail mutant bound to ADP
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 9gnq
Q-score: 0.54
Atherton J, Chegkazi MS, Peirano E, Pozzer LS, Foran T, Steiner RA
bioRxiv (2024) [ DOI: doi:10.1101/2024.11.11.622991 ]
- Beta tubulin (Complex from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Alpha-tubulin (Complex from Sus scrofa)
- Kinesin-1 heavy chain (44 kDa, Protein from Homo sapiens)
- 13pf microtubule bound to kif5bmonexxx in the presence of adp (246 MDa, Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Taxol (853 Da, Ligand)
- Kif5bmonexxx bound with adp (Complex from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Sus scrofa)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 8va2
Q-score: 0.36
Aher A, Urnavicius L, Xue A, Neselu K, Kapoor TM
Nat Struct Mol Biol (2024) 31 pp. 1124-1133 [ DOI: doi:10.1038/s41594-024-01264-z Pubmed: 38609661 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin alpha-1b chain (51 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin gamma-1 chain (50 kDa, Protein from Homo sapiens)
- Gamma-tubulin and alpha-tubulin (Complex from Trichoplusia ni)
S. pombe microtubule decorated with Cut7 motor domain in the AMPPNP state
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5mlv
Q-score: 0.355
von Loeffelholz O, Pena A, Drummond DR, Cross R, Moores CA
J Mol Biol (2019) 431 pp. 864-872 [ Pubmed: 30659798 DOI: doi:10.1016/j.jmb.2019.01.011 ]
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1 chain (51 kDa, Protein from Schizosaccharomyces pombe 972h-)
- 7,11-dihydroxy-8,8,10,12,16-pentamethyl-3-[1-methyl-2-(2-methyl-thiazol-4-yl)vinyl]-4,17-dioxabicyclo[14.1.0]heptadecane-5,9-dione (507 Da, Ligand)
- Cut7 (Complex from Schizosaccharomyces pombe 972)
- Cut7 mtd decorated s. pombe microtubule stabilized with epothilone-b (Complex)
- Kinesin-like protein cut7 (47 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Alpha tubulin, beta tubulin (Complex from Schizosaccharomyces pombe 972)
Near-atomic cryo-EM structure of PRC1 bound to the microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5kmg
Q-score: 0.41
Kellogg EH, Howes S, Ti SC, Ramirez-Aportela E, Kapoor TM, Chacon P, Nogales E
PNAS (2016) 113 pp. 9430-9439 [ Pubmed: 27493215 DOI: doi:10.1073/pnas.1609903113 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Protein regulator of cytokinesis 1 (15 kDa, Protein from Homo sapiens)
- Tubulin beta chain (48 kDa, Protein from Sus scrofa)
- Peloruside a (548 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (49 kDa, Protein from Sus scrofa)
- Ternary complex of prc1 fragment (prc1-sc), alpha-tubulin, and beta-tubulin. (Complex)
Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syc
Q-score: 0.426
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Peloruside a (548 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of peloruside-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5sye
Q-score: 0.372
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Peloruside a (548 Da, Ligand)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of taxol- and peloruside-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syf
Q-score: 0.39
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of taxol-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
Cryo-EM reconstruction of zampanolide-bound microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syg
Q-score: 0.303
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- (2z,4e)-n-[(s)-[(1s,2e,5s,8e,10z,17s)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide (497 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of zampanolide-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)