Subpellicular microtubule from detergent-extract Toxoplasma gondii cells
Resolution: 6.7 Å
EM Method: Subtomogram averaging
Fitted PDBs: 7tns
Q-score: 0.118
Sun SY, Segev-Zarko LA, Chen M, Pintilie GD, Schmid MF, Ludtke SJ, Boothroyd JC, Chiu W
PNAS (2022) 119 [ DOI: doi:10.1073/pnas.2111661119 Pubmed: 35121661 ]
- Tubulin beta chain (50 kDa, Protein from Toxoplasma gondii)
- Microtubule complex of alpha-tubulin and beta-tubulin with intra-lumenal proteins (Cellular component from Toxoplasma gondii)
- Tubulin alpha chain (50 kDa, Protein from Toxoplasma gondii)
- Pdi family protein (21 kDa, Protein from Toxoplasma gondii)
- Pdi family protein (24 kDa, Protein from Toxoplasma gondii)
- Microtubule associated protein spm1 (38 kDa, Protein from Toxoplasma gondii)
Complex of yeast cytoplasmic dynein MTBD-High and MT with DTT
Resolution: 3.62 Å
EM Method: Helical reconstruction
Fitted PDBs: 6kiq
Q-score: 0.455
Nishida N, Komori Y, Takarada O, Watanabe A, Tamura S, Kubo S, Shimada I, Kikkawa M
Nat Commun (2020) 11 pp. 1038-1038 [ DOI: doi:10.1038/s41467-020-14842-8 Pubmed: 32098965 ]
- Alpha tubulin (45 kDa, Protein from Sus scrofa)
- Dynein heavy chain, cytoplasmic (15 kDa, Protein from Saccharomyces cerevisiae S288c)
- Mtbd-high/mt complex with dtt (Cell from Sus scrofa)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
S. pombe microtubule decorated with Cut7 motor domain in the AMPPNP state
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 5mlv
Q-score: 0.355
von Loeffelholz O, Pena A, Drummond DR, Cross R, Moores CA
J Mol Biol (2019) 431 pp. 864-872 [ Pubmed: 30659798 DOI: doi:10.1016/j.jmb.2019.01.011 ]
- Magnesium ion (24 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Tubulin beta chain (49 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1 chain (51 kDa, Protein from Schizosaccharomyces pombe 972h-)
- 7,11-dihydroxy-8,8,10,12,16-pentamethyl-3-[1-methyl-2-(2-methyl-thiazol-4-yl)vinyl]-4,17-dioxabicyclo[14.1.0]heptadecane-5,9-dione (507 Da, Ligand)
- Cut7 (Complex from Schizosaccharomyces pombe 972)
- Cut7 mtd decorated s. pombe microtubule stabilized with epothilone-b (Complex)
- Kinesin-like protein cut7 (47 kDa, Protein from Schizosaccharomyces pombe 972h-)
- Alpha tubulin, beta tubulin (Complex from Schizosaccharomyces pombe 972)
Near-atomic cryo-EM structure of PRC1 bound to the microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5kmg
Q-score: 0.41
Kellogg EH, Howes S, Ti SC, Ramirez-Aportela E, Kapoor TM, Chacon P, Nogales E
PNAS (2016) 113 pp. 9430-9439 [ Pubmed: 27493215 DOI: doi:10.1073/pnas.1609903113 ]
- Magnesium ion (24 Da, Ligand)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Protein regulator of cytokinesis 1 (15 kDa, Protein from Homo sapiens)
- Tubulin beta chain (48 kDa, Protein from Sus scrofa)
- Peloruside a (548 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Tubulin alpha-1b chain (49 kDa, Protein from Sus scrofa)
- Ternary complex of prc1 fragment (prc1-sc), alpha-tubulin, and beta-tubulin. (Complex)
Near-atomic resolution cryo-EM reconstruction of peloruside-stabilized microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syc
Q-score: 0.426
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- Magnesium ion (24 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Peloruside a (548 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of peloruside-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5sye
Q-score: 0.372
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Peloruside a (548 Da, Ligand)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of taxol- and peloruside-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
High-resolution cryo-EM reconstruction of Taxol-stabilized microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syf
Q-score: 0.39
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Taxol (853 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of taxol-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
Cryo-EM reconstruction of zampanolide-bound microtubule
Resolution: 3.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 5syg
Q-score: 0.303
Kellogg EH, Hejab NM, Howes S, Northcote P, Miller JH, Diaz JF, Downing KH, Nogales E
J Mol Biol (2017) 429 pp. 633-646 [ Pubmed: 28104363 DOI: doi:10.1016/j.jmb.2017.01.001 ]
- (2z,4e)-n-[(s)-[(1s,2e,5s,8e,10z,17s)-3,11-dimethyl-19-methylidene-7,13-dioxo-6,21-dioxabicyclo[15.3.1]henicosa-2,8,10-trien-5-yl](hydroxy)methyl]hexa-2,4-dienamide (497 Da, Ligand)
- Tubulin alpha chain (48 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Tubulin beta chain (47 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Ternary complex of zampanolide-stabilized microtubule lattice, alpha-tubulin, and beta-tubulin (Complex from Sus scrofa)
Electron microscopy of the complex formed by chaperones TBCE and TBCB and alpha-tubulin
Serna M, Carranza G, Martin-Benito J, Janowski R, Canals A, Coll M, Zabala JC, Valpuesta JM
J Cell Sci (2015) 128 pp. 1824-1834 [ Pubmed: 25908846 DOI: doi:10.1242/jcs.167387 ]
- Alpha-tubulin (50 kDa, Protein from Bos taurus)
- Tubulin binding cofactor b (27 kDa, Protein from Homo sapiens)
- Tubulin binding cofactor e (59 kDa, Protein from Homo sapiens)
- Ternary complex of alpha-tubulin, tbce and tbcb (137 kDa, Sample)
Goulet A, Major J, Jun Y, Gross SP, Rosenfeld SS, Moores CA
PNAS (2014) 111 pp. 1837-1842 [ DOI: doi:10.1073/pnas.1319848111 Pubmed: 24449904 ]