Structure of AT118-H Nanobody Antagonist in Complex with the Angiotensin II Type I Receptor
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 8th3
Q-score: 0.532
Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC
Nat Chem Biol (2024) 20 pp. 1577-1585 [ DOI: doi:10.1038/s41589-024-01620-6 Pubmed: 38744986 ]
- Bag2 anti-bril fab heavy chain (24 kDa, Protein from synthetic construct)
- Bag2 anti-bril fab light chain (23 kDa, Protein from synthetic construct)
- Cholesterol hemisuccinate (486 Da, Ligand)
- At118-h nanobody, type-1 angiotensin ii receptor, soluble cytochrome b562 complex (65 kDa, Protein from Homo sapiens)
- At118-h nanobody antagonist in complex with the angiotensin ii type i receptor bril fusion, anti-bril fab, and anti-fab nanobody (Complex from Homo sapiens)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8th4
Q-score: 0.495
Skiba MA, Sterling SM, Rawson S, Zhang S, Xu H, Jiang H, Nemeth GR, Gilman MSA, Hurley JD, Shen P, Staus DP, Kim J, McMahon C, Lehtinen MK, Rockman HA, Barth P, Wingler LM, Kruse AC
Nat Chem Biol (2024) 20 pp. 1577-1585 [ DOI: doi:10.1038/s41589-024-01620-6 Pubmed: 38744986 ]
- Bag2 anti-bril fab heavy chain (24 kDa, Protein from synthetic construct)
- Bag2 anti-bril fab light chain (23 kDa, Protein from synthetic construct)
- Type-1 angiotensin ii receptor soluble cytochrome b562 complex (49 kDa, Protein from Homo sapiens)
- At118-l nanobody (15 kDa, Protein from synthetic construct)
- At118-l nanobody antagonist in complex with the angiotensin ii type i receptor bril fusion protein, anti-bril fab, anti-fab nanobody, and losartan (Complex from Homo sapiens)
- [2-butyl-5-chloranyl-3-[[4-[2-(2h-1,2,3,4-tetrazol-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol (422 Da, Ligand)
Resolution: 2.98 Å
EM Method: Single-particle
Fitted PDBs: 7yud
Q-score: 0.53
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- Nbfab-h-chain (26 kDa, Protein from synthetic construct)
- Tc-nb8 (14 kDa, Protein from Lama glama)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Spns2 nanobody complex (Complex from Lama glama)
- (2~{s})-2-azanyl-4-(4-octylphenyl)-2-[[oxidanyl-bis(oxidanylidene)-$l^{6}-phosphanyl]oxymethyl]butan-1-ol (387 Da, Ligand)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Nbfab l-chain (23 kDa, Protein from synthetic construct)
Resolution: 3.29 Å
EM Method: Single-particle
Fitted PDBs: 7yuf
Q-score: 0.458
Duan Y, Leong NCP, Zhao J, Zhang Y, Nguyen DT, Ha HTT, Wang N, Xia R, Xu Z, Ma Z, Qian Y, Yin H, Zhu X, Zhang A, Guo C, Xia Y, Nguyen LN, He Y
Cell Res (2024) 34 pp. 177-180 [ DOI: doi:10.1038/s41422-023-00913-0 Pubmed: 38123825 ]
- Antifab nanobody (13 kDa, Protein from Lama glama)
- Tc-nb8 (14 kDa, Protein from Lama glama)
- Spns2 nanobody complex (Complex from Lama glama)
- Nbfab h-chain (26 kDa, Protein from synthetic construct)
- Sphingosine-1-phosphate transporter spns2 (58 kDa, Protein from Homo sapiens)
- Nbfab l-chain (23 kDa, Protein from synthetic construct)