Structure of the mu opioid receptor bound to the antagonist nanobody NbE
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8qot
Q-score: 0.413
Yu J, Kumar A, Zhang X, Martin C, Van Holsbeeck K, Raia P, Koehl A, Laeremans T, Steyaert J, Manglik A, Ballet S, Boland A, Stoeber M
Nat Commun (2024) 15 pp. 8687-8687 [ Pubmed: 39384768 DOI: doi:10.1038/s41467-024-52947-6 ]
- Nabfab hc (28 kDa, Protein from synthetic construct)
- Anti-fab nanobody (17 kDa, Protein from Lama glama)
- Nanobody e (nbe) (18 kDa, Protein from Lama glama)
- Mor-nbe-nabfab-antifab complex (120 kDa, Complex from Mus musculus)
- Mu-type opioid receptor (53 kDa, Protein from Mus musculus)
- Nabfab lc (25 kDa, Protein from synthetic construct)
Resolution: 3.47 Å
EM Method: Single-particle
Fitted PDBs: 7php
Q-score: 0.505
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Water (18 Da, Ligand)
- Complex of vibrio cholerae mate transporter norm, chimeric nanobody norm-nb17_4, nabfab, and anti-fab nanobody (Complex from Vibrio cholerae RC385)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Norm-nb17_4 (14 kDa, Protein from synthetic construct)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Multidrug resistance protein norm (52 kDa, Protein from Vibrio cholerae RC385)
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Anti-fab nanobody (Complex)
- Complex of vibrio cholerae mate transporter norm, chimeric nanobody norm-nb17_4, nabfab, and anti-fab nanobody (Complex from Vibrio cholerae RC385)
- Nabfab hc (Complex from synthetic construct)
- Nabfab lc (Complex from synthetic construct)
- Multidrug resistance protein norm (Complex from synthetic construct)
- Norm-nb17_4 (Complex from Lama glama)
Resolution: 8.45 Å
EM Method: Single-particle
Fitted PDBs: 7phq
Q-score: 0.111
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Water (18 Da, Ligand)
- Complex of staphylococcus capitis divalent metal ion transporter (dmt), chimeric nanobody dmtnb16_4, nabfab, and anti-fab nanobody (Complex from synthetic construct)
- Dmt-nb16_4 (14 kDa, Protein from synthetic construct)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Divalent metal cation transporter mnth (47 kDa, Protein from Staphylococcus capitis)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Nabfab hc (25 kDa, Protein from synthetic construct)
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 7pij
Q-score: 0.422
Bloch JS, Mukherjee S, Kowal J, Filippova EV, Niederer M, Pardon E, Steyaert J, Kossiakoff AA, Locher KP
PNAS (2021) 118 [ Pubmed: 34782475 DOI: doi:10.1073/pnas.2115435118 ]
- Water (18 Da, Ligand)
- Complex of staphylococcus capitis divalent metal ion transporter (dmt), chimeric nanobody dmtnb16_4, nabfab, and anti-fab nanobody (Complex from synthetic construct)
- Nabfab lc (23 kDa, Protein from synthetic construct)
- Divalent metal cation transporter mnth (47 kDa, Protein from Staphylococcus capitis)
- Anti-fab nanobody (13 kDa, Protein from Lama glama)
- Nabfab hc (25 kDa, Protein from synthetic construct)
- Dmtnb16_4 (14 kDa, Protein from synthetic construct)