Arp2/3 branch junction complex, ADP state
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8uxw
Q-score: 0.585
Chavali SS, Chou SZ, Cao W, Pollard TD, De La Cruz EM, Sindelar CV
Nat Commun (2024) 15 pp. 2059-2059 [ DOI: doi:10.1038/s41467-024-46179-x Pubmed: 38448439 ]
- Arp2/3 branch complex (adp state) (Complex)
- Actin, alpha skeletal muscle (42 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 2 (44 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 3 (47 kDa, Protein from Schizosaccharomyces pombe)
- Arp2/3 complex (Complex from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from Schizosaccharomyces pombe)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin (Cellular component from Gallus gallus)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from Schizosaccharomyces pombe)
Arp2/3 branch junction complex, BeFx state
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 8uxx
Q-score: 0.519
Chavali SS, Chou SZ, Cao W, Pollard TD, De La Cruz EM, Sindelar CV
Nat Commun (2024) 15 pp. 2059-2059 [ DOI: doi:10.1038/s41467-024-46179-x Pubmed: 38448439 ]
- Actin, alpha skeletal muscle (42 kDa, Protein from Oryctolagus cuniculus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Water (18 Da, Ligand)
- Actin-related protein 2 (44 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 3 (47 kDa, Protein from Schizosaccharomyces pombe)
- Arp2/3 complex (Complex from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from Schizosaccharomyces pombe)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Actin (Cellular component from Gallus gallus)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Schizosaccharomyces pombe)
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from Schizosaccharomyces pombe)
- Arp2/3 branch complex (adp-befx state) (Complex)
Straight actin filament from Arp2/3 branch junction sample (ADP-BeFx)
Resolution: 3.6 Å
EM Method: Helical reconstruction
Fitted PDBs: 8uz1
Q-score: 0.489
Chavali SS, Chou SZ, Cao W, Pollard TD, De La Cruz EM, Sindelar CV
Nat Commun (2024) 15 pp. 2059-2059 [ DOI: doi:10.1038/s41467-024-46179-x Pubmed: 38448439 ]
- Magnesium ion (24 Da, Ligand)
- Arp2/3 complex (Complex from Schizosaccharomyces pombe)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Beryllium trifluoride ion (66 Da, Ligand)
- Actin (Cellular component from Gallus gallus)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Arp2/3 branch complex (adp-befx state) (Complex)
Cryo-EM structure of Cortactin-bound to Arp2/3 complex
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8tah
Q-score: 0.514
Fregoso FE, Boczkowska M, Rebowski G, Carman PJ, van Eeuwen T, Dominguez R
Nat Commun (2023) 14 pp. 6894-6894 [ DOI: doi:10.1038/s41467-023-42229-y Pubmed: 37898612 ]
- Cortactin nta bound to arp2/3 complex (223 kDa, Complex from Bos taurus)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Actin-related protein 3 (47 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 3 (20 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Bos taurus)
- Src substrate cortactin (8 kDa, Protein from Mus musculus)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 2 (34 kDa, Protein from Bos taurus)
- Actin-related protein 2 (44 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 1a (41 kDa, Protein from Bos taurus)
Cryo-EM Structure of a Transition State of Arp2/3 Complex Activation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7t5q
Q-score: 0.385
van Eeuwen T, Boczkowska M, Rebowski G, Carman PJ, Fregoso FE, Dominguez R
PNAS (2023) 120 pp. e2306165120-e2306165120 [ DOI: doi:10.1073/pnas.2306165120 Pubmed: 37549294 ]
- Magnesium ion (24 Da, Ligand)
- Actin skeletal muscle (Complex from Oryctolagus cuniculus)
- F-actin-capping protein subunit beta/actin nucleation-promoting factor wasl chimera (37 kDa, Protein from Mus musculus)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- F-actin-capping protein subunit alpha-1/actin nucleation-promoting factor wasl chimera (39 kDa, Protein from Mus musculus)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-related protein 3 (47 kDa, Protein from Bos taurus)
- Bovine arp2/3 complex and actin, capz (248 kDa, Complex)
- Actin-related protein 2/3 complex subunit 3 (20 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Bos taurus)
- Bovine arp2/3 (Complex from Bos taurus)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from Bos taurus)
- Actin, alpha skeletal muscle (41 kDa, Protein from Oryctolagus cuniculus)
- Actin-related protein 2/3 complex subunit 2 (34 kDa, Protein from Bos taurus)
- Actin-related protein 2 (44 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 1a (41 kDa, Protein from Bos taurus)
- F-actin-capping protein (Complex from Homo sapiens)
Cryo-EM structure of S. pombe Arp2/3 complex in the branch junction
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8e9b
Q-score: 0.439
Chou SZ, Chatterjee M, Pollard TD
PNAS (2022) 119 pp. e2206722119-e2206722119 [ Pubmed: 36442092 DOI: doi:10.1073/pnas.2206722119 ]
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from fission yeast)
- Actin-related protein 2/3 complex subunit 3 (19 kDa, Protein from fission yeast)
- S. pombe arp2/3 complex in actin branch junction (Complex from Schizosaccharomyces pombe)
- Actin, alpha skeletal muscle (41 kDa, Protein from chicken)
- Actin-related protein 3 (47 kDa, Protein from fission yeast)
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- S. pombe arp2/3 complex in actin branch junction (Complex)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Actin-related protein 2/3 complex subunit 5 (16 kDa, Protein from fission yeast)
- Actin-related protein 2 (44 kDa, Protein from fission yeast)
- Chicken skeletal muscle actin in branch junction (Complex from Gallus gallus)
- Actin-related protein 2/3 complex subunit 1 (41 kDa, Protein from fission yeast)
- Actin-related protein 2/3 complex subunit 2 (37 kDa, Protein from Fission yeast)
Cryo-EM structure of Arpin-bound Arp2/3 complex
Resolution: 3.24 Å
EM Method: Single-particle
Fitted PDBs: 7jpn
Q-score: 0.433
Fregoso FE, van Eeuwen T, Simanov G, Rebowski G, Boczkowska M, Zimmet A, Gautreau AM, Dominguez R
Nat Commun (2022) 13 pp. 628-628 [ Pubmed: 35110533 DOI: doi:10.1038/s41467-022-28112-2 ]
- Magnesium ion (24 Da, Ligand)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Arpin (Complex from Homo sapiens)
- Arpin (3 kDa, Protein from Homo sapiens)
- Actin-related protein 3 (46 kDa, Protein from Bos taurus)
- Bovine arp2/3 complex with arpin (248 kDa, Complex)
- Actin-related protein 2/3 complex subunit 3 (20 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 4 (19 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 5 (15 kDa, Protein from Bos taurus)
- Bovine arp2/3 (Complex from Bos taurus)
- Actin-related protein 2/3 complex subunit 1b (41 kDa, Protein from Bos taurus)
- Actin-related protein 2/3 complex subunit 2 (34 kDa, Protein from Bos taurus)
- Actin-related protein 2 (42 kDa, Protein from Bos taurus)