CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (inhibited by DDM)
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7zm7
Q-score: 0.598
Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W
Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]
- Nadh dehydrogenase-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase 49 kda subunit-like protein (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase (ubiquinone)-like protein (43 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (82 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Zinc ion (65 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase (ubiquinone)-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase 30.4 kda subunit-like protein (33 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufv2 of nadh-ubiquinone oxidoreductase (complex i) (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase b14 subunit-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Mitochondrial nadh:ubiquinone oxidoreductase in ddm (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
- Water (18 Da, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Flavin mononucleotide (456 Da, Ligand)
- Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Oxidoreductase-like domain-containing protein (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Iron/sulfur cluster (351 Da, Ligand)
- Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Cardiolipin (1 kDa, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Resolution: 2.76 Å
EM Method: Single-particle
Fitted PDBs: 7zm8
Q-score: 0.604
Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W
Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ DOI: doi:10.1126/sciadv.adc9952 Pubmed: 36427319 ]
- Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase (ubiquinone)-like protein (89 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Cardiolipin (1 kDa, Ligand)
- Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Mitochondrial nadh:ubiquinone oxidoreductase in ddm (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Water (18 Da, Ligand)
- Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2)
Resolution: 2.75 Å
EM Method: Single-particle
Fitted PDBs: 7zmb
Q-score: 0.598
Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W
Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]
- Nadh dehydrogenase-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase 49 kda subunit-like protein (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase (ubiquinone)-like protein (43 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Mitochondrial nadh:ubiquinone oxidoreductase in lmng (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (82 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase (ubiquinone)-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase 30.4 kda subunit-like protein (33 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufv2 of nadh-ubiquinone oxidoreductase (complex i) (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase b14 subunit-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Water (18 Da, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Flavin mononucleotide (456 Da, Ligand)
- Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Oxidoreductase-like domain-containing protein (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Iron/sulfur cluster (351 Da, Ligand)
- Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Cardiolipin (1 kDa, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 2) - membrane arm
Resolution: 2.83 Å
EM Method: Single-particle
Fitted PDBs: 7zme
Q-score: 0.596
Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W
Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]
- Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Mitochondrial nadh:ubiquinone oxidoreductase in lmng (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Water (18 Da, Ligand)
- Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Cardiolipin (1 kDa, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase (ubiquinone)-like protein (89 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1)
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 7zmg
Q-score: 0.667
Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W
Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]
- Nadh dehydrogenase-like protein (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase 49 kda subunit-like protein (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase (ubiquinone)-like protein (43 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Mitochondrial nadh:ubiquinone oxidoreductase in lmng (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (82 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (55 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase (ubiquinone)-like protein (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Zinc ion (65 Da, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (18 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase 30.4 kda subunit-like protein (33 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufv2 of nadh-ubiquinone oxidoreductase (complex i) (35 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase b14 subunit-like protein (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Water (18 Da, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (25 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Flavin mononucleotide (456 Da, Ligand)
- Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (29 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (23 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Oxidoreductase-like domain-containing protein (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Iron/sulfur cluster (351 Da, Ligand)
- Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Cardiolipin (1 kDa, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
CryoEM structure of mitochondrial complex I from Chaetomium thermophilum (state 1) - membrane arm
Resolution: 2.47 Å
EM Method: Single-particle
Fitted PDBs: 7zmh
Q-score: 0.672
Laube E, Meier-Credo J, Langer JD, Kuhlbrandt W
Sci Adv (2022) 8 pp. eadc9952-eadc9952 [ Pubmed: 36427319 DOI: doi:10.1126/sciadv.adc9952 ]
- Complex i-esss (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 1 (41 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Mitochondrial nadh:ubiquinone oxidoreductase in lmng (970 kDa, Complex from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 5 (75 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb2 of nadh-ubiquinone oxidoreductase (complex i) (5 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufs5 of nadh-ubiquinone oxidoreductase (complex i) (87 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (14 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Acyl carrier protein (15 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb3 of nadh-ubiquinone oxidoreductase (complex i) (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4 (60 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 4l (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa1 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase subunit 2 (64 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Water (18 Da, Ligand)
- Subunit ndufc2 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- 1,2-distearoyl-sn-glycerophosphoethanolamine (748 Da, Ligand)
- Lauryl maltose neopentyl glycol (1 kDa, Ligand)
- Nadh-ubiquinone oxidoreductase-like protein (21 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Complex i-b22 (11 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 3 (16 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb10 of nadh-ubiquinone oxidoreductase (complex i) (12 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh-ubiquinone oxidoreductase chain 6 (24 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufa3 of nadh-ubiquinone oxidoreductase (complex i) (9 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb6 of nadh-ubiquinone oxidoreductase (complex i) (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb4 of nadh-ubiquinone oxidoreductase (complex i) (8 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Cardiolipin (1 kDa, Ligand)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase (ubiquinone)-like protein (89 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (10 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
- Subunit ndufb5 of nadh-ubiquinone oxidoreductase (complex i) (20 kDa, Protein from Chaetomium thermophilum var. thermophilum DSM 1495)
Complex I from Ovis aries at pH5.5, Open state
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 7zdj
Q-score: 0.547
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ DOI: doi:10.1038/s41586-022-05199-7 Pubmed: 36104567 ]
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (14 kDa, Protein from sheep)
- Complex i-b14.7 (14 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (75 kDa, Protein from sheep)
- Complex i-mnll (6 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from sheep)
- Acyl carrier protein (17 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (18 kDa, Protein from sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from sheep)
- Zinc ion (65 Da, Ligand)
- Complex i-b22 (21 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from sheep)
- Complex i-sgdh (16 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (9 kDa, Protein from sheep)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from sheep)
- Complex i-mwfe (8 kDa, Protein from sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from sheep)
- Complex i-9kd (5 kDa, Protein from sheep)
- Complex i-esss (11 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit b4 (14 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from sheep)
- Complex i-b9 (8 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (36 kDa, Protein from sheep)
- Flavin mononucleotide (456 Da, Ligand)
- Complex i-49kd (52 kDa, Protein from sheep)
- Complex i subunit b13 (13 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (23 kDa, Protein from sheep)
- Complex i-aggg (7 kDa, Protein from sheep)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Complex i-20kd (17 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (10 kDa, Protein from sheep)
- Iron/sulfur cluster (351 Da, Ligand)
- Complex i from ovis aries at ph5.5, open state (1 MDa, Complex from Ovis aries)
- Complex i-b14 (13 kDa, Protein from sheep)
- Complex i-pdsw (20 kDa, Protein from sheep)
- Complex i-30kd (24 kDa, Protein from sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from sheep)
- Complex i-b18 (14 kDa, Protein from sheep)
- Complex i-23kd (20 kDa, Protein from sheep)
- Complex i-kfyi (5 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (47 kDa, Protein from sheep)
- Myristic acid (228 Da, Ligand)
- Complex i-b12 (8 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from sheep)
Complex I from Ovis aries at pH9, Open state
Resolution: 3.88 Å
EM Method: Single-particle
Fitted PDBs: 7zdp
Q-score: 0.437
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ DOI: doi:10.1038/s41586-022-05199-7 Pubmed: 36104567 ]
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (14 kDa, Protein from sheep)
- Complex i-b14.7 (14 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (75 kDa, Protein from sheep)
- Complex i-mnll (6 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from sheep)
- Acyl carrier protein (17 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (18 kDa, Protein from sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from sheep)
- Zinc ion (65 Da, Ligand)
- Complex i-b22 (21 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from sheep)
- Complex i-sgdh (16 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (9 kDa, Protein from sheep)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from sheep)
- Complex i-mwfe (8 kDa, Protein from sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from sheep)
- Complex i-9kd (5 kDa, Protein from sheep)
- Complex i-esss (11 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit b4 (14 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from sheep)
- Complex i-b9 (8 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (36 kDa, Protein from sheep)
- Flavin mononucleotide (456 Da, Ligand)
- Complex i-49kd (52 kDa, Protein from sheep)
- Complex i subunit b13 (13 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (23 kDa, Protein from sheep)
- Complex i-aggg (7 kDa, Protein from sheep)
- Complex i from ovis aries at ph9, open state (1 MDa, Complex from Ovis aries)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Complex i-20kd (17 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (10 kDa, Protein from sheep)
- Iron/sulfur cluster (351 Da, Ligand)
- Complex i-b14 (13 kDa, Protein from sheep)
- Complex i-pdsw (20 kDa, Protein from sheep)
- Complex i-30kd (24 kDa, Protein from sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from sheep)
- Adenosine monophosphate (347 Da, Ligand)
- Complex i-b18 (14 kDa, Protein from sheep)
- Complex i-23kd (20 kDa, Protein from sheep)
- Complex i-kfyi (5 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (47 kDa, Protein from sheep)
- Myristic acid (228 Da, Ligand)
- Complex i-b12 (8 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from sheep)
Complex I from Ovis aries at pH9, Closed state
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7zeb
Q-score: 0.452
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ Pubmed: 36104567 DOI: doi:10.1038/s41586-022-05199-7 ]
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from sheep)
- Complex i-pdsw (20 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (17 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (23 kDa, Protein from sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit b4 (14 kDa, Protein from sheep)
- Flavin mononucleotide (456 Da, Ligand)
- Complex i-esss (11 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (49 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from sheep)
- Acyl carrier protein (17 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (10 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from sheep)
- Myristic acid (228 Da, Ligand)
- Nadh:ubiquinone oxidoreductase subunit a9 (38 kDa, Protein from sheep)
- Complex i-9kd (5 kDa, Protein from sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from sheep)
- Adenosine monophosphate (347 Da, Ligand)
- Complex i-sgdh (16 kDa, Protein from sheep)
- Complex i-b9 (8 kDa, Protein from sheep)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from sheep)
- Complex i-23kd (24 kDa, Protein from sheep)
- Complex i-b22 (21 kDa, Protein from sheep)
- Complex i-mwfe (8 kDa, Protein from sheep)
- Complex i from ovis aries at ph9, closed state (1 MDa, Complex from Ovis aries)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (36 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from sheep)
- Complex i-b14 (13 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (18 kDa, Protein from sheep)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (9 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (75 kDa, Protein from sheep)
- Complex i-b14.7 (14 kDa, Protein from sheep)
- Complex i-b12 (8 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (47 kDa, Protein from sheep)
- Complex i-aggg (7 kDa, Protein from sheep)
- Zinc ion (65 Da, Ligand)
- Complex i-mnll (6 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from sheep)
- Complex i-30kd (24 kDa, Protein from sheep)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from sheep)
- Complex i-kfyi (5 kDa, Protein from sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
Cryo-EM structure of Polytomella Complex-I (membrane arm)
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 7ar9
Q-score: 0.565
Klusch N, Senkler J, Yildiz O, Kuhlbrandt W, Braun HP
Plant Cell (2021) 33 pp. 2072-2091 [ DOI: doi:10.1093/plcell/koab092 Pubmed: 33768254 ]
- B12 (6 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ashi (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Cal (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B9 (4 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd4l (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mnll (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Pdsw (18 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd1 (31 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Cardiolipin (1 kDa, Ligand)
- B14.5b (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd5 (58 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B14.7 (22 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Pgiv (11 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Mwfe (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Kfyi (12 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- 15 kda (8 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop8 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Ca2 (33 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd6 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B22 (14 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Phosphatidylethanolamine (734 Da, Ligand)
- Ca3 (24 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Aggg (7 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Water (18 Da, Ligand)
- B15 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B18 (10 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd4 (48 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop5 (15 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Esss (19 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- B16.6 (16 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Sdap1 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop4 (9 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nuop7 (13 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd2 (41 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- (7s)-4-hydroxy-n,n,n-trimethyl-9-oxo-7-[(palmitoyloxy)methyl]-3,5,8-trioxa-4-phosphahexacosan-1-aminium 4-oxide (763 Da, Ligand)
- Polytomella complex i - membrane arm (Complex from Polytomella sp. Pringsheim 198.80)
- C1-fdx (23 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Unknown (4 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Unknown (3 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- Nd3 (17 kDa, Protein from Polytomella sp. Pringsheim 198.80)
- S-[2-({n-[(2r)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate (540 Da, Ligand)