Complex I from Ovis aries at pH5.5, Open state
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 7zdj
Q-score: 0.547
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ DOI: doi:10.1038/s41586-022-05199-7 Pubmed: 36104567 ]
- Complex i-pdsw (20 kDa, Protein from sheep)
- Complex i subunit b13 (13 kDa, Protein from sheep)
- Complex i-b14.7 (14 kDa, Protein from sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Complex i-9kd (5 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (36 kDa, Protein from sheep)
- Complex i-b22 (21 kDa, Protein from sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Complex i-aggg (7 kDa, Protein from sheep)
- Complex i-sgdh (16 kDa, Protein from sheep)
- Complex i-kfyi (5 kDa, Protein from sheep)
- Complex i-b12 (8 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (18 kDa, Protein from sheep)
- Complex i-b18 (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (9 kDa, Protein from sheep)
- Complex i-23kd (20 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit b4 (14 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from sheep)
- Complex i-b9 (8 kDa, Protein from sheep)
- Acyl carrier protein (17 kDa, Protein from sheep)
- Complex i-30kd (24 kDa, Protein from sheep)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (47 kDa, Protein from sheep)
- Complex i from ovis aries at ph5.5, open state (1 MDa, Complex from Ovis aries)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from sheep)
- Complex i-49kd (52 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from sheep)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Complex i-mnll (6 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (10 kDa, Protein from sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from sheep)
- Complex i-mwfe (8 kDa, Protein from sheep)
- Complex i-20kd (17 kDa, Protein from sheep)
- Myristic acid (228 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from sheep)
- Flavin mononucleotide (456 Da, Ligand)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Complex i-b14 (13 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from sheep)
- Complex i-esss (11 kDa, Protein from sheep)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (75 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (23 kDa, Protein from sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from sheep)
Complex I from Ovis aries at pH9, Open state
Resolution: 3.88 Å
EM Method: Single-particle
Fitted PDBs: 7zdp
Q-score: 0.437
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ DOI: doi:10.1038/s41586-022-05199-7 Pubmed: 36104567 ]
- Complex i-pdsw (20 kDa, Protein from sheep)
- Complex i subunit b13 (13 kDa, Protein from sheep)
- Complex i-b14.7 (14 kDa, Protein from sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Complex i-9kd (5 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (36 kDa, Protein from sheep)
- Complex i from ovis aries at ph9, open state (1 MDa, Complex from Ovis aries)
- Complex i-b22 (21 kDa, Protein from sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Complex i-aggg (7 kDa, Protein from sheep)
- Complex i-sgdh (16 kDa, Protein from sheep)
- Complex i-kfyi (5 kDa, Protein from sheep)
- Complex i-b12 (8 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (18 kDa, Protein from sheep)
- Complex i-b18 (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (9 kDa, Protein from sheep)
- Complex i-23kd (20 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit b4 (14 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from sheep)
- Complex i-b9 (8 kDa, Protein from sheep)
- Acyl carrier protein (17 kDa, Protein from sheep)
- Complex i-30kd (24 kDa, Protein from sheep)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (47 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from sheep)
- Complex i-49kd (52 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (43 kDa, Protein from sheep)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Adenosine monophosphate (347 Da, Ligand)
- Complex i-mnll (6 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (10 kDa, Protein from sheep)
- Mitochondrial complex i, b14.5a subunit (12 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from sheep)
- Complex i-mwfe (8 kDa, Protein from sheep)
- Complex i-20kd (17 kDa, Protein from sheep)
- Myristic acid (228 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from sheep)
- Flavin mononucleotide (456 Da, Ligand)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Complex i-b14 (13 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from sheep)
- Complex i-esss (11 kDa, Protein from sheep)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (75 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (23 kDa, Protein from sheep)
- Mitochondrial complex i, b17 subunit (15 kDa, Protein from sheep)
Complex I from Ovis aries at pH9, Closed state
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7zeb
Q-score: 0.452
Kravchuk V, Petrova O, Kampjut D, Wojciechowska-Bason A, Breese Z, Sazanov L
Nature (2022) 609 pp. 808-814 [ DOI: doi:10.1038/s41586-022-05199-7 Pubmed: 36104567 ]
- Complex i-pdsw (20 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial (49 kDa, Protein from sheep)
- Complex i-b14.7 (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 (13 kDa, Protein from sheep)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- Complex i-9kd (5 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 subunit c2 (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 10, mitochondrial (36 kDa, Protein from sheep)
- Complex i-b22 (21 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit a9 (38 kDa, Protein from sheep)
- S-[2-({n-[(2s)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] tetradecanethioate (568 Da, Ligand)
- Complex i-aggg (7 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 6 (15 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 7 (14 kDa, Protein from sheep)
- Complex i-sgdh (16 kDa, Protein from sheep)
- Complex i-kfyi (5 kDa, Protein from sheep)
- Complex i-b12 (8 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 beta subcomplex subunit 8, mitochondrial (18 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 4, mitochondrial (14 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 2 (9 kDa, Protein from sheep)
- Nadh:ubiquinone oxidoreductase subunit b4 (14 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4 (52 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 (20 kDa, Protein from sheep)
- 1,2-diacyl-sn-glycero-3-phosphocholine (790 Da, Ligand)
- Complex i-b9 (8 kDa, Protein from sheep)
- Acyl carrier protein (17 kDa, Protein from sheep)
- Complex i-30kd (24 kDa, Protein from sheep)
- Potassium ion (39 Da, Ligand)
- Nadh-ubiquinone oxidoreductase chain 3 (13 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 7, mitochondrial (17 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 4l (10 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] flavoprotein 1, mitochondrial (47 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 13 (16 kDa, Protein from sheep)
- Complex i-23kd (24 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 5 (68 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 6 (19 kDa, Protein from sheep)
- Nadph dihydro-nicotinamide-adenine-dinucleotide phosphate (745 Da, Ligand)
- Adenosine monophosphate (347 Da, Ligand)
- Complex i-mnll (6 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 6, mitochondrial (10 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] iron-sulfur protein 5 (12 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 1 (35 kDa, Protein from sheep)
- Complex i-mwfe (8 kDa, Protein from sheep)
- Myristic acid (228 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 7 (12 kDa, Protein from sheep)
- Nadh dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 12 (17 kDa, Protein from sheep)
- Flavin mononucleotide (456 Da, Ligand)
- Complex i-b14 (13 kDa, Protein from sheep)
- Nadh-ubiquinone oxidoreductase chain 2 (39 kDa, Protein from sheep)
- 1,4-dihydronicotinamide adenine dinucleotide (665 Da, Ligand)
- Complex i-esss (11 kDa, Protein from sheep)
- Iron/sulfur cluster (351 Da, Ligand)
- Nadh-ubiquinone oxidoreductase 75 kda subunit, mitochondrial (75 kDa, Protein from sheep)
- Complex i from ovis aries at ph9, closed state (1 MDa, Complex from Ovis aries)
- Nadh dehydrogenase [ubiquinone] flavoprotein 2, mitochondrial (23 kDa, Protein from sheep)